cDNA Clone Ubiquitin

• Plasmid: pTXB1 HALO Ubiquitin intein CBD
• Parent Plasmid: pTXB1
• Expressed: HALO Ubiquitin intein CBD
• Genbank: -
• Tag: HALO
• Tag: Intein

Sequence of Insert

GCTAGCCTCGCGAGTCGACGGCGGCCGCATGCAGATCTTCGTCAAGACGTTAACCGGTAAAACCATAACTCTAGAAGTGGAACCGAGCGATACCATCGAAAACGTGAAAGCGAAAATCCAGGATAAAGAAGGCATCCCGCCGGATCAGCAGCGTCTGATCTTTGCGGGCAAACAGCTGGAAGATGGCCGCACCCTGTCTGATTATAACATCCAGAAAGAGTCGACCCTGCATCTGGTCTTAAGACTGCGTGGCGCTCTTCTTGCATCACG

Amino Acid Sequence

MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGASSRVDGGRMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGCITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPVLADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAVIQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYYAKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHATGLTGLNSGLTTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNVPALWQLQ*

• Antibiotic: Amp
• Full Snapgene Map: pTXB1 HALO Ubiquitin intein CBD121.85 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU47812
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: ubiquitin sub-cloned Not Sap and then put into frame and excess bases after ubiquitin removed
• Low Temp: No