cDNA Clone PCMTD1

• Plasmid: pSc-B-PCMTD1
• Parent Plasmid: pSC-B
• Genbank: Q96MG8-1 NM_052937.3 Gene 115294
• Species: Human

Sequence of Insert

ggatccATGGGAGGAGCTGTGAGTGCTGGGGAAGATAATGATGACTTAAT
TGATAATTTAAAAGAAGCTCAGTATATTCGTACTGAAAGAGTGGAGCAAG
CCTTCAGAGCGATTGATCGTGGAGATTACTATTTGGAAGGCTACAGAGAC
AATGCTTACAAAGACTTAGCCTGGAAGCATGGAAACATCCACTTGTCAGC
ACCTTGCATTTATTCTGAAGTTATGGAAGCATTGAAACTTCAACCAGGAT
TGTCTTTTCTTAACCTGGGAAGTGGAACCGGATATTTAAGTACAATGGTG
GGCTTAATTTTAGGTCCTTTTGGAATAAATCATGGGATTGAGCTTCATTC
AGATGTGGTGGAATATGCCAAGGAAAAACTGGAGAGCTTCATCAAAAATA
GTGATAGCTTTGATAAATTTGAGTTCTGTGAACCTGCATTTGTTGTTGGT
AATTGCCTCCAGATAGCTTCTGACAGTCATCAGTATGATCGAATTTATTG
TGGAGCTGGAGTACAGAAAGACCATGAAAACTACATGAAAATATTACTAA
AAGTTGGAGGCATATTAGTCATGCCTATAGAGGATCAGTTAACACAGATT
ATGCGAACTGGACAGAACACTTGGGAAAGTAAAAATATCCTTGCTGTTTC
ATTTGCTCCACTTGTGCAACCAAGTAAGAATGATAATGGCAAACCAGATT
CTGTGGGACTCCCTCCCTGTGCTGTCAGGAATCTACAGGACTTGGCTCGT
ATTTACATTCGACGCACACTTAGAAATTTCATAAATGATGAGATGCAGGC
CAAGGGGATTCCTCAAAGGGCTCCACCCAAAAGGAAAAGAAAGAGAGTTA
AACAGAGAATTAACACTTACGTATTTGTGGGTAATCAGCTTATTCCTCAG
CCTCTAGACAGTGAAGAGGATGAAAAAATGGAAGAGGATAACAAAGAAGA
GGAGGAAAAAGATCACAATGAAGCAATGAAGCCAGAGGAGCCACCTCAAA
ATTTACTGAGAGAAAAAATCATGAAGCTGCCCCTCCCTGAATCTTTAAAA
GCTTACTTGACATATTTTAGAGACAAATAAgcggccgc

Amino Acid Sequence

MGGAVSAGEDNDDLIDNLKEAQYIRTERVEQAFRAIDRGDYYLEGYRDNA
YKDLAWKHGNIHLSAPCIYSEVMEALKLQPGLSFLNLGSGTGYLSTMVGL
ILGPFGINHGIELHSDVVEYAKEKLESFIKNSDSFDKFEFCEPAFVVGNC
LQIASDSHQYDRIYCGAGVQKDHENYMKILLKVGGILVMPIEDQLTQIMR
TGQNTWESKNILAVSFAPLVQPSKNDNGKPDSVGLPPCAVRNLQDLARIY
IRRTLRNFINDEMQAKGIPQRAPPKRKRKRVKQRINTYVFVGNQLIPQPL
DSEEDEKMEEDNKEEEEKDHNEAMKPEEPPQNLLREKIMKLPLPESLKAY
LTYFRDK*

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU28708
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: silent change at V169 (GTG > GTA) compared to NCBI ref. N312 restored with oligos shown
• Low Temp: No