cDNA Clone BICC1/A6NGY7-1

• Plasmid: pCS2+DU-HA-BICC1
• Parent Plasmid: pCS2+DU-HA
• Expressed: HA-BICC1
• Genbank: Gene 80114 NM_001080512.2 → NP_001073981.1
• Tag: HA
• Species: Human

Sequence of Insert

agatctATGGCCGCCCAGGGAGAGCCCGGCTACCTGGCGGCGCAGTCGGA
CCCCGGCTCCAACAGCGAGCGCAGCACCGACTCCCCAGTGCCCGGCTCCG
AGGACGACTTGGTCGCCGGGGCGACCCTGCACAGCCCGGAGTGGAGCGAG
GAGCGCTTCCGCGTGGACAGGAAGAAACTTGAGGCCATGTTACAAGCTGC
TGCTGAAGGGAAAGGCAGAAGTGGGGAAGACTTTTTTCAAAAGATCATGG
AGGAAACAAATACGCAGATTGCTTGGCCATCAAAACTGAAGATCGGAGCC
AAATCCAAGAAAGATCCCCATATTAAGGTTTCTGGAAAGAAAGAAGATGT
TAAAGAAGCCAAGGAAATGATCATGTCTGTCTTAGACACAAAAAGCAATC
GAGTCACACTGAAGATGGATGTTTCACATACAGAACATTCACATGTAATC
GGCAAAGGTGGCAACAATATTAAAAAAGTGATGGAAGAAACCGGATGCCA
TATCCACTTTCCAGATTCCAACAGGAATAACCAAGCAGAAAAAAGCAACC
AGGTATCTATAGCGGGACAACCAGCAGGAGTAGAATCTGCCCGAGTTAGA
ATTCGGGAGCTGCTTCCTTTGGTGCTGATGTTTGAGCTACCAATTGCTGG
AATTCTTCAACCGGTTCCTGATCCTAATTCCCCCTCTATTCAGCATATAT
CACAAACGTACAATATTTCAGTATCATTTAAACAGCGTTCCCGAATGTAT
GGTGCTACTGTCATAGTACGAGGGTCTCAGAATAACACTAGTGCTGTGAA
GGAAGGAACTGCCATGCTGTTAGAACATCTTGCTGGGAGCTTAGCATCAG
CTATTCCTGTGAGCACACAACTAGATATTGCAGCTCAACATCATCTCTTT
ATGATGGGTCGAAATGGGAGCAACATCAAACATATCATGCAGAGAACAGG
TGCTCAGATCCACTTTCCTGATCCCAGTAATCCACAAAAGAAATCTACCG
TCTACCTCCAGGGCACCATTGAGTCTGTCTGTCTTGCAAGGCAATATCTC
ATGGGTTGTCTTCCTCTTGTGTTGATGTTTGATATGAAGGAAGAAATTGA
AGTAGATCCACAATTCATTGCGCAGTTGATGGAACAGCTTGATGTCTTCA
TCAGTATTAAACCAAAGCCCAAACAGCCAAGCAAGTCTGTGATTGTGAAA
AGTGTTGAGCGAAATGCCTTAAATATGTATGAAGCAAGGAAATGTCTCCT
CGGACTTGAAAGCAGTGGGGTTACCATAGCAACCAGTCCATCCCCAGCAT
CCTGCCCTGCCGGCCTGGCATGTCCCAGCCTGGATATCTTAGCTTCAGCA
GGCCTTGGACTCACTGGACTAGGTCTTTTGGGACCCACCACCTTATCTCT
GAACACTTCAACAACCCCAAACTCACTCTTGAATGCTCTTAATAGCTCAG
TCAGTCCTTTGCAAAGTCCAAGTTCTGGTACACCCAGCCCCACATTATGG
GCACCCCCACTTGCTAATACTTCAAGTGCCACAGGTTTTTCTGCTATACC
ACACCTTATGATTCCATCTACTGCCCAAGCCACATTAACTAATATTTTGT
TGTCTGGAGTGCCCACCTATGGGCACACAGCTCCATCTCCCCCTCCTGGC
TTGACTCCTGTTGATGTCCATATCAACAGTATGCAGACCGAAGGCAAAAA
AATCTCTGCTGCTTTAAATGGACATGCACAGTCTCCAGATATAAAATATG
GTGCAATATCCACTTCATCACTTGGAGAAAAAGTGCTGAGTGCAAATCAC
GGGGATCCGTCCATCCAGACAAGTGGGTCTGAGCAGACATCTCCCAAATC
AAGCCCCACTGAAGGTTGTAATGATGCTTTTGTTGAAGTAGGCATGCCTC
GAAGTCCTTCCCATTCTGGGAATGCTGGTGACTTGAAACAGATGATGTGT
CCCTCCAAGGTTTCCTGTGCCAAAAGGCAGACAGTGGAACTATTGCAAGG
CACGAAAAACTCACACTTACACAGCACTGACAGGTTGCTCTCAGACCCTG
AACTGAGTGCTACCGAAAGCCCTTTGGCTGACAAGAAGGCTCCAGGGAGT
GAGCGCGCTGCAGAGAGGGCAGCAGCTGCCCAGCAAAACTCCGAAAGGGC
CCACCTTGCTCCACGGTCATCATATGTCAACATGCAGGCATTTGACTATG
AACAGAAGAAGCTATTAGCCACCAAAGCTATGTTAAAGAAACCAGTGGTG
ACGGAGGTCAGAACGCCCACAAATACCTGGAGTGGCCTGGGTTTTTCTAA
ATCCATGCCAGCTGAAACTATCAAGGAGTTGAGAAGGGCCAATCATGTGT
CCTATAAGCCCACAATGACAACCACTTATGAGGGCTCATCCATGTCCCTT
TCACGGTCCAACAGTCGTGAGCACTTGGGAGGTGGAAGCGAATCTGATAA
CTGGAGAGACCGAAATGGAATTGGACCTGGAAGTCATAGTGAATTTGCAG
CTTCTATTGGCAGCCCTAAGCGTAAACAAAACAAATCAACGGAACACTAT
CTCAGCAGTAGCAATTACATGGACTGCATTTCCTCGCTGACAGGAAGCAA
TGGCTGTAACCTAAATAGCTCTTTCAAAGGTTCTGACCTCCCTGAGCTCT
TCAGCAAACTGGGCCTGGGCAAATACACAGATGTTTTCCAGCAACAAGAG
ATCGATCTTCAGACATTCCTCACTCTCACAGATCAGGATCTGAAGGAGCT
GGGAATAACTACTTTTGGTGCCAGGAGGAAAATGCTGCTTGCAATTTCAG
AACTAAATAAAAACCGAAGAAAGCTTTTTGAACCGCCAAATGCACGCACC
TCTTTCCTGGAAGGTGGAGCGAGTGGAAGGCTACCCCGTCAGTATCACTC
AGACATTGCTAGTGTCAGTGGCCGCTGGTAGgcggccgc

Amino Acid Sequence

MYPYDVPDYAGSMAAQGEPGYLAAQSDPGSNSERSTDSPVPGSEDDLVAG
ATLHSPEWSEERFRVDRKKLEAMLQAAAEGKGRSGEDFFQKIMEETNTQI
AWPSKLKIGAKSKKDPHIKVSGKKEDVKEAKEMIMSVLDTKSNRVTLKMD
VSHTEHSHVIGKGGNNIKKVMEETGCHIHFPDSNRNNQAEKSNQVSIAGQ
PAGVESARVRIRELLPLVLMFELPIAGILQPVPDPNSPSIQHISQTYNIS
VSFKQRSRMYGATVIVRGSQNNTSAVKEGTAMLLEHLAGSLASAIPVSTQ
LDIAAQHHLFMMGRNGSNIKHIMQRTGAQIHFPDPSNPQKKSTVYLQGTI
ESVCLARQYLMGCLPLVLMFDMKEEIEVDPQFIAQLMEQLDVFISIKPKP
KQPSKSVIVKSVERNALNMYEARKCLLGLESSGVTIATSPSPASCPAGLA
CPSLDILASAGLGLTGLGLLGPTTLSLNTSTTPNSLLNALNSSVSPLQSP
SSGTPSPTLWAPPLANTSSATGFSAIPHLMIPSTAQATLTNILLSGVPTY
GHTAPSPPPGLTPVDVHINSMQTEGKKISAALNGHAQSPDIKYGAISTSS
LGEKVLSANHGDPSIQTSGSEQTSPKSSPTEGCNDAFVEVGMPRSPSHSG
NAGDLKQMMCPSKVSCAKRQTVELLQGTKNSHLHSTDRLLSDPELSATES
PLADKKAPGSERAAERAAAAQQNSERAHLAPRSSYVNMQAFDYEQKKLLA
TKAMLKKPVVTEVRTPTNTWSGLGFSKSMPAETIKELRRANHVSYKPTMT
TTYEGSSMSLSRSNSREHLGGGSESDNWRDRNGIGPGSHSEFAASIGSPK
RKQNKSTEHYLSSSNYMDCISSLTGSNGCNLNSSFKGSDLPELFSKLGLG
KYTDVFQQQEIDLQTFLTLTDQDLKELGITTFGARRKMLLAISELNKNRR
KLFEPPNARTSFLEGGASGRLPRQYHSDIASVSGRW*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29154
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Contains listed SNP Ser943Pro and silent change at L869 (TTA > CTA) compared to NCBI ref.; Parental 28162
• Low Temp: No