cDNA Clone HERC5

• Synonyms: HERC5 HECT and RLD domain containing E3 ubiquitin protein ligase 5
• Plasmid: pSc-B-HERC5
• Parent Plasmid: pSC-B
• Genbank: NM_016323.3 → NP_057407.2 Q9UII4 Gene 51191
• Species: Human

Sequence of Insert

ggatccATGGAGCGGAGGTCGCGGAGGAAGTCGCGGCGCAACGGGCGCTC
GACCGCGGGCAAGGCCGCCGCGACCCAGCCCGCGAAGTCTCCGGGCGCAC
AGCTCTGGCTCTTTCCCAGCGCCGCGGGCCTCCACCGCGCGCTGCTCCGG
AGGGTGGAGGTGACGCGCCAACTCTGCTGCTCGCCGGGGCGCCTCGCGGT
CTTGGAACGCGGCGGGGCGGGCGTCCAGGTTCACCAGCTGCTCGCCGGGA
GCGGCGGCGCCCGGACGCCGAAATGCATTAAATTAGGAAAAAACATGAAG
ATACATTCCGTGGACCAAGGAGCAGAGCACATGCTGATTCTCTCATCAGA
TGGAAAACCATTTGAGTATGACAACTATAGCATGAAACATCTAAGGTTTG
AAAGCATTTTACAAGAAAAAAAAATAATTCAGATCACATGTGGAGATTAC
CATTCTCTTGCACTCTCAAAAGGTGGTGAGCTTTTTGCCTGGGGACAGAA
CCTGCATGGGCAGCTTGGAGTTGGAAGGAAATTTCCCTCAACCACCACAC
CACAGATTGTGGAGCACCTCGCAGGAGTACCCTTGGCTCAGATTTCTGCC
GGAGAAGCCCACAGCATGGCCTTATCCATGTCTGGCAACATTTATTCATG
GGGAAAAAATGAATGTGGACAACTAGGCCTGGGCCACACTGAGAGTAAAG
ATGATCCATCCCTTATTGAAGGACTAGACAATCAGAAAGTTGAATTTGTC
GCTTGTGGTGGCTCTCACAGTGCCCTACTCACACAGGATGGGCTGCTGTT
TACTTTCGGTGCTGGAAAACATGGGCAACTTGGTCATAATTCAACACAGA
ATGAGCTAAGACCCTGTTTGGTGGCTGAGCTTGTTGGGTATAGAGTGACT
CAGATAGCATGTGGAAGGTGGCACACACTTGCCTATGTTTCTGATTTGGG
AAAGGTCTTTTCCTTTGGTTCTGGAAAAGATGGACAACTGGGAAATGGTG
GAACACGTGACCAGCTGATGCCGCTTCCAGTGAAAGTATCATCAAGTGAA
GAACTCAAACTTGAAAGCCATACCTCAGAAAAGGAGTTAATAATGATTGC
TGGAGGGAATCAAAGCATTTTGCTCTGGATAAAGAAAGAGAATTCATATG
TTAATCTGAAGAGGACAATTCCTACTCTGAATGAAGGGACTGTAAAGAGA
TGGATTGCTGATGTGGAGACTAAACGGTGGCAGAGCACAAAAAGGGAAAT
CCAAGAGATATTTTCATCTCCTGCTTGTCTAACTGGAAGTTTTTTAAGGA
AAAGAAGAACTACAGAAATGATGCCTGTTTATTTGGACTTAAATAAAGCA
AGAAACATCTTCAAGGAGTTAACCCAAAAGGACTGGATTACTAACATGAT
AACCACCTGCCTCAAAGATAATCTGCTCAAAAGACTTCCATTTCATTCTC
CACCCCAAGAAGCTTTAGAAATTTTCTTCCTTCTCCCAGAATGTCCTATG
ATGCATATTTCCAACAACTGGGAGAGCCTTGTGGTTCCATTTGCAAAGGT
TGTTTGTAAAATGAGTGACCAGTCTTCACTGGTTCTGGAAGAGTATTGGG
CAACTCTGCAAGAATCCACTTTCAGCAAACTGGTCCAGATGTTTAAAACA
GCCGTCATATGCCAGTTGGATTACTGGGATGAAAGTGCTGAGGAGAATGG
TAATGTTCAAGCTCTCCTAGAAATGTTGAAGAAGCTGCACAGGGTAAACC
AGGTGAAATGTCAACTACCTGAAAGTATTTTCCAAGTAGACGAACTCTTG
CACCGTCTCAATTTTTTTGTAGAAGTATGCAGAAGGTACTTGTGGAAAAT
GACTGTGGACGCTTCAGAAAATGTACAATGCTGCGTCATATTCAGTCACT
TTCCATTTATCTTTAATAATCTGTCGAAAATTAAACTACTACATACAGAC
ACACTTTTAAAAATAGAGAGTAAAAAACATAAAGCTTATCTTAGGTCGGC
AGCAATTGAGGAAGAAAGAGAGTCTGAATTCGCTTTGAGGCCCACGTTTG
ATCTAACAGTCAGAAGGAATCACTTGATTGAGGATGTTTTGAATCAGCTA
AGTCAATTTGAGAATGAAGACCTGAGGAAAGAGTTATGGGTTTCATTTAG
TGGAGAAATTGGGTATGACCTCGGAGGAGTCAAGAAAGAGTTCTTCTACT
GTCTGTTTGCAGAGATGATCCAGCCGGAATATGGGATGTTCATGTATCCT
GAAGGGGCTTCCTGCATGTGGTTTCCTGTCAAGCCTAAATTTGAGAAGAA
AAGATACTTCTTTTTTGGGGTTCTATGTGGACTTTCCCTGTTCAATTGCA
ATGTTGCCAACCTCCCTTTCCCACTGGCACTGTTTAAGAAACTTTTGGAC
CAAATGCCATCATTGGAAGACTTGAAAGAACTCAGTCCTGATTTGGGAAA
GAATTTGCAAACACTTCTGGATGATGAAGGTGATAACTTTGAGGAAGTAT
TTTACATCCATTTTAATGTGCACTGGGACAGAAACGACACAAACTTAATT
CCTAATGGAAGTAGCATAACTGTCAACCAGACTAACAAGAGAGACTATGT
TTCTAAGTATATCAATTACATTTTCAACGACTCTGTAAAGGCGGTTTATG
AAGAATTTCGGAGAGGATTTTATAAAATGTGCGACGAAGACATTATCAAA
TTATTCCACCCCGAAGAACTGAAGGATGTGATTGTTGGAAATACAGATTA
TGATTGGAAAACATTTGAAAAGAATGCACGTTATGAACCAGGATATAACA
GTTCACATCCCACCATAGTGATGTTTTGGAAGGCTTTCCACAAATTGACT
CTGGAAGAAAAGAAAAAATTCCTTGTATTTCTTACAGGAACTGACAGACT
ACAAATGAAAGATTTAAATAATATGAAAATAACATTTTGCTGTCCTGAAA
GTTGGAATGAAAGAGACCCTATAAGAGCACTGACATGTTTCAGTGTCCTC
TTCCTCCCTAAATATTCTACAATGGAAACAGTTGAAGAAGCGCTTCAAGA
AGCCATCAACAACAACAGAGGATTTGGCTGAgcggccgc

Amino Acid Sequence

MERRSRRKSRRNGRSTAGKAAATQPAKSPGAQLWLFPSAAGLHRALLRRV
EVTRQLCCSPGRLAVLERGGAGVQVHQLLAGSGGARTPKCIKLGKNMKIH
SVDQGAEHMLILSSDGKPFEYDNYSMKHLRFESILQEKKIIQITCGDYHS
LALSKGGELFAWGQNLHGQLGVGRKFPSTTTPQIVEHLAGVPLAQISAGE
AHSMALSMSGNIYSWGKNECGQLGLGHTESKDDPSLIEGLDNQKVEFVAC
GGSHSALLTQDGLLFTFGAGKHGQLGHNSTQNELRPCLVAELVGYRVTQI
ACGRWHTLAYVSDLGKVFSFGSGKDGQLGNGGTRDQLMPLPVKVSSSEEL
KLESHTSEKELIMIAGGNQSILLWIKKENSYVNLKRTIPTLNEGTVKRWI
ADVETKRWQSTKREIQEIFSSPACLTGSFLRKRRTTEMMPVYLDLNKARN
IFKELTQKDWITNMITTCLKDNLLKRLPFHSPPQEALEIFFLLPECPMMH
ISNNWESLVVPFAKVVCKMSDQSSLVLEEYWATLQESTFSKLVQMFKTAV
ICQLDYWDESAEENGNVQALLEMLKKLHRVNQVKCQLPESIFQVDELLHR
LNFFVEVCRRYLWKMTVDASENVQCCVIFSHFPFIFNNLSKIKLLHTDTL
LKIESKKHKAYLRSAAIEEERESEFALRPTFDLTVRRNHLIEDVLNQLSQ
FENEDLRKELWVSFSGEIGYDLGGVKKEFFYCLFAEMIQPEYGMFMYPEG
ASCMWFPVKPKFEKKRYFFFGVLCGLSLFNCNVANLPFPLALFKKLLDQM
PSLEDLKELSPDLGKNLQTLLDDEGDNFEEVFYIHFNVHWDRNDTNLIPN
GSSITVNQTNKRDYVSKYINYIFNDSVKAVYEEFRRGFYKMCDEDIIKLF
HPEELKDVIVGNTDYDWKTFEKNARYEPGYNSSHPTIVMFWKAFHKLTLE
EKKKFLVFLTGTDRLQMKDLNNMKITFCCPESWNERDPIRALTCFSVLFL
PKYSTMETVEEALQEAINNNRGFG*

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU29695
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent change at Leu786 (CTT > CTC)
• Low Temp: No