cDNA Clone HERC5

• Synonyms: HERC5 HECT and RLD domain containing E3 ubiquitin protein ligase 5
• Plasmid: pEX-GST-HERC5 E692-G1024 (end)
• Parent Plasmid: pEX-GST
• Expressed: HERC5 E692-G1024 (end)-GST
• Genbank: NM_016323.3 → NP_057407.2 Q9UII4 Gene 51191
• Tag: GST
• Species: Human

Sequence of Insert

ggatccGAGGATGTTTTGAATCAGCTAAGTCAATTTGAGAATGAAGACCT
GAGGAAAGAGTTATGGGTTTCATTTAGTGGAGAAATTGGGTATGACCTCG
GAGGAGTCAAGAAAGAGTTCTTCTACTGTCTGTTTGCAGAGATGATCCAG
CCGGAATATGGGATGTTCATGTATCCTGAAGGGGCTTCCTGCATGTGGTT
TCCTGTCAAGCCTAAATTTGAGAAGAAAAGATACTTCTTTTTTGGGGTTC
TATGTGGACTTTCCCTGTTCAATTGCAATGTTGCCAACCTCCCTTTCCCA
CTGGCACTGTTTAAGAAACTTTTGGACCAAATGCCATCATTGGAAGACTT
GAAAGAACTCAGTCCTGATTTGGGAAAGAATTTGCAAACACTTCTGGATG
ATGAAGGTGATAACTTTGAGGAAGTATTTTACATCCATTTTAATGTGCAC
TGGGACAGAAACGACACAAACTTAATTCCTAATGGAAGTAGCATAACTGT
CAACCAGACTAACAAGAGAGACTATGTTTCTAAGTATATCAATTACATTT
TCAACGACTCTGTAAAGGCGGTTTATGAAGAATTTCGGAGAGGATTTTAT
AAAATGTGCGACGAAGACATTATCAAATTATTCCACCCCGAAGAACTGAA
GGATGTGATTGTTGGAAATACAGATTATGATTGGAAAACATTTGAAAAGA
ATGCACGTTATGAACCAGGATATAACAGTTCACATCCCACCATAGTGATG
TTTTGGAAGGCTTTCCACAAATTGACTCTGGAAGAAAAGAAAAAATTCCT
TGTATTTCTTACAGGAACTGACAGACTACAAATGAAAGATTTAAATAATA
TGAAAATAACATTTTGCTGTCCTGAAAGTTGGAATGAAAGAGACCCTATA
AGAGCACTGACATGTTTCAGTGTCCTCTTCCTCCCTAAATATTCTACAAT
GGAAACAGTTGAAGAAGCGCTTCAAGAAGCCATCAACAACAACAGAGGAT
TTGGCgcggccgc

Amino Acid Sequence

MGSEDVLNQLSQFENEDLRKELWVSFSGEIGYDLGGVKKEFFYCLFAEMI
QPEYGMFMYPEGASCMWFPVKPKFEKKRYFFFGVLCGLSLFNCNVANLPF
PLALFKKLLDQMPSLEDLKELSPDLGKNLQTLLDDEGDNFEEVFYIHFNV
HWDRNDTNLIPNGSSITVNQTNKRDYVSKYINYIFNDSVKAVYEEFRRGF
YKMCDEDIIKLFHPEELKDVIVGNTDYDWKTFEKNARYEPGYNSSHPTIV
MFWKAFHKLTLEEKKKFLVFLTGTDRLQMKDLNNMKITFCCPESWNERDP
IRALTCFSVLFLPKYSTMETVEEALQEAINNNRGFGAAALEVLFQGPLSR
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29736
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent change at Leu786 (CTT > CTC); Parental 30890
• Low Temp: No