cDNA Clone HERC5

• Synonyms: HERC5 HECT and RLD domain containing E3 ubiquitin protein ligase 5
• Plasmid: pcDNA5-FRT/TO-HERC5 E692-G1024 (end)
• Parent Plasmid: pcDNA5-FRT/TO
• Expressed: HERC5 E692-G1024 (end)-GST
• Genbank: NM_016323.3 → NP_057407.2 Q9UII4 Gene 51191
• Species: Human

Sequence of Insert

ggatccATGGAGGATGTTTTGAATCAGCTAAGTCAATTTGAGAATGAAGA
CCTGAGGAAAGAGTTATGGGTTTCATTTAGTGGAGAAATTGGGTATGACC
TCGGAGGAGTCAAGAAAGAGTTCTTCTACTGTCTGTTTGCAGAGATGATC
CAGCCGGAATATGGGATGTTCATGTATCCTGAAGGGGCTTCCTGCATGTG
GTTTCCTGTCAAGCCTAAATTTGAGAAGAAAAGATACTTCTTTTTTGGGG
TTCTATGTGGACTTTCCCTGTTCAATTGCAATGTTGCCAACCTCCCTTTC
CCACTGGCACTGTTTAAGAAACTTTTGGACCAAATGCCATCATTGGAAGA
CTTGAAAGAACTCAGTCCTGATTTGGGAAAGAATTTGCAAACACTTCTGG
ATGATGAAGGTGATAACTTTGAGGAAGTATTTTACATCCATTTTAATGTG
CACTGGGACAGAAACGACACAAACTTAATTCCTAATGGAAGTAGCATAAC
TGTCAACCAGACTAACAAGAGAGACTATGTTTCTAAGTATATCAATTACA
TTTTCAACGACTCTGTAAAGGCGGTTTATGAAGAATTTCGGAGAGGATTT
TATAAAATGTGCGACGAAGACATTATCAAATTATTCCACCCCGAAGAACT
GAAGGATGTGATTGTTGGAAATACAGATTATGATTGGAAAACATTTGAAA
AGAATGCACGTTATGAACCAGGATATAACAGTTCACATCCCACCATAGTG
ATGTTTTGGAAGGCTTTCCACAAATTGACTCTGGAAGAAAAGAAAAAATT
CCTTGTATTTCTTACAGGAACTGACAGACTACAAATGAAAGATTTAAATA
ATATGAAAATAACATTTTGCTGTCCTGAAAGTTGGAATGAAAGAGACCCT
ATAAGAGCACTGACATGTTTCAGTGTCCTCTTCCTCCCTAAATATTCTAC
AATGGAAACAGTTGAAGAAGCGCTTCAAGAAGCCATCAACAACAACAGAG
GATTTGGCTGAgcggccgc

Amino Acid Sequence

MEDVLNQLSQFENEDLRKELWVSFSGEIGYDLGGVKKEFFYCLFAEMIQP
EYGMFMYPEGASCMWFPVKPKFEKKRYFFFGVLCGLSLFNCNVANLPFPL
ALFKKLLDQMPSLEDLKELSPDLGKNLQTLLDDEGDNFEEVFYIHFNVHW
DRNDTNLIPNGSSITVNQTNKRDYVSKYINYIFNDSVKAVYEEFRRGFYK
MCDEDIIKLFHPEELKDVIVGNTDYDWKTFEKNARYEPGYNSSHPTIVMF
WKAFHKLTLEEKKKFLVFLTGTDRLQMKDLNNMKITFCCPESWNERDPIR
ALTCFSVLFLPKYSTMETVEEALQEAINNNRGFG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29749
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 41459; Silent change at Leu786 (CTT > CTC)
• Low Temp: No