cDNA Clone UBE2A

• Plasmid: p6HisMEX-UBE2A C152S
• Expressed: His-MBP-UBE2A C152S
• Tag: 6His
• Tag: MBP
• Species: Human

Sequence of Insert

AGATCTATGTCCACCCCGGCTCGGCGGCGCCTCATGCGGGACTTCAAGAG
GTTGCAGGAGGATCCTCCAGCCGGAGTCAGCGGGGCTCCGTCCGAGAACA
ACATAATGGTGTGGAACGCGGTCATTTTCGGGCCTGAAGGGACCCCGTTT
GAGGATGGAACATTTAAACTTACAATAGAATTCACTGAAGAATATCCAAA
TAAACCACCTACAGTTAGATTTGTCTCTAAGATGTTCCATCCAAATGTCT
ATGCAGATGGTAGTATATGTCTGGACATACTTCAGAACCGTTGGAGTCCA
ACCTATGATGTGTCTTCCATTCTAACATCCATACAGTCTCTGTTGGATGA
ACCCAATCCCAATAGTCCAGCAAACAGCCAGGCTGCTCAGCTGTACCAGG
AGAACAAACGGGAATATGAAAAGCGTGTTTCTGCAATAGTAGAACAAAGC
TGGCGTGATTCTTGAGCGGCCGC

Amino Acid Sequence

MHHHHHHMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDK
LEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPF
TWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAK
GKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNY
GVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEA
VNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYA
VRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFL
EVLFQGPLGSMSTPARRRLMRDFKRLQEDPPAGVSGAPSENNIMVWNAVI
FGPEGTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHPNVYADGSICLD
ILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKR
VSAIVEQSWRDS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29996
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: sub cloned into Bam-Not backbone of 26341
• Low Temp: No