cDNA Clone HERC4

• Plasmid: pcDNA5-FRT/TO-HA-HERC4 isoform a
• Parent Plasmid: pcDNA5-FRT/TO-HA
• Expressed: HA-HERC4 isoform a
• Genbank: NM_022079.3 → NP_071362.1
• Tag: HA
• Species: Human

Sequence of Insert

gaattcATGTTGTGCTGGGGAAATGCATCCTTTGGGCAGCTAGGTTTGGG
TGGAATTGATGAAGAAATTGTACTAGAGCCCAGAAAAAGTGACTTCTTTA
TAAATAAAAGGGTCCGAGATGTAGGATGTGGACTCAGACATACTGTGTTT
GTTCTGGATGATGGAACAGTGTACACATGTGGATGTAATGATCTAGGACA
GCTAGGTCATGAAAAATCCAGAAAGAAACCAGAGCAGGTTGTTGCCCTGG
ATGCCCAAAATATTGTAGCTGTTTCATGTGGAGAAGCTCATACGTTAGCG
CTAAATGACAAAGGCCAGGTGTATGCTTGGGGTCTCGATTCTGATGGACA
GCTTGGCCTGGTAGGATCAGAGGAATGCATCAGAGTACCCAGAAATATTA
AAAGTTTGTCAGATATCCAGATTGTACAGGTTGCTTGTGGTTACTATCAT
TCACTTGCACTTTCTAAAGCAAGTGAAGTCTTCTGTTGGGGACAGAATAA
ATATGGCCAATTGGGTTTAGGTACTGACTGTAAAAAGCAAACTTCACCGC
AGCTGCTTAAGTCTTTGCTTGGAATCCCTTTCATGCAAGTTGCAGCAGGA
GGAGCCCATAGTTTTGTACTCACCCTTTCTGGAGCTATCTTTGGATGGGG
ACGCAACAAGTTTGGTCAGCTAGGTCTTAATGATGAAAATGATAGGTATG
TTCCTAATTTACTAAAGTCACTAAGATCTCAGAAAATAGTTTATATTTGT
TGTGGAGAAGATCATACTGCTGCTCTAACCAAGGAAGGTGGAGTGTTTAC
TTTTGGAGCTGGAGGGTATGGTCAGTTGGGCCATAATTCTACCAGTCATG
AAATAAACCCAAGGAAAGTTTTTGAACTTATGGGAAGCATTGTCACTGAG
ATTGCTTGTGGACGGCAGCACACTTCTGCTTTTGTTCCTTCATCAGGACG
AATTTACTCTTTTGGGCTTGGTGGTAATGGGCAGCTGGGAACCGGTTCAA
CAAGCAACAGGAAAAGtCCCTTTACTGTAAAAGGAAATTGGTACCCCTAT
AATGGGCAGTGTCTACCAGATATTGATTCTGAAGAATATTTCTGTGTAAA
AAGAATTTTCTCAGGGGGAGATCAAAGCTTTTCACATTACTCTAGTCCCC
AGAACTGTGGGCCACCAGATGACTTCAGATGTCCCAATCCGACAAAGCAG
ATCTGGACAGTGAATGAAGCTCTAATTCAGAAATGGCTGAGCTATCCTTC
TGGAAGGTTTCCTGTGGAGATAGCCAATGAGATAGATGGAACGTTTTCTT
CCTCTGGTTGCCTAAATGGAAGTTTTTTAGCTGTTAGCAATGATGATCAC
TATAGAACAGGTACCAGATTTTCAGGGGTTGATATGAATGCTGCTAGGCT
TTTATTCCACAAACTTATACAACCTGATCATCCGCAGATATCTCAGCAGG
TGGCAGCTAGTTTGGAAAAGAATCTTATTCCTAAACTGACTAGCTCCTTA
CCTGATGTTGAAGCATTGAGGTTTTATCTTACTCTACCAGAATGTCCCCT
GATGAGTGATTCCAACAATTTCACAACAATAGCAATTCCCTTTGGTACAG
CTCTTGTGAACCTAGAAAAGGCACCACTGAAAGTACTTGAAAACTGGTGG
TCAGTACTTGAACCTCCACTATTCCTCAAGATAGTAGAACTTTTTAAGGA
AGTTGTGGTACATCTTTTGAAACTCTACAAGATCGGTATTCCCCCTTCTG
AAAGAAGAATTTTCAACAGTTTTCTTCATACTGCATTAAAGGTTTTAGAA
ATACTACATAGGGTAAATGAGAAAATGGGACAGATTATACAGTATGATAA
ATTTTATATACATGAAGTACAAGAATTGATAGACATAAGAAATGATTATA
TCAACTGGGTCCAACAGCAGGCCTATGGAATGGATGTCAACCATGGATTA
ACTGAGTTGGCAGATATCCCTGTTACAATCTGTACATATCCATTTGTATT
TGATGCCCAAGCAAAAACTACTCTGTTACAGACCGATGCAGTCTTACAGA
TGCAGATGGCTATTGATCAGGCCCACAGGCAGAATGTCTCCTCTCTTTTT
CTCCCAGTGATTGAATCTGTGAATCCCTGCTTAATTCTAGTGGTGCGTAG
AGAAAATATTGTAGGAGATGCAATGGAAGTCCTTAGGAAAACAAAGAACA
TAGATTACAAGAAGCCACTCAAGGTTATATTTGTTGGAGAAGATGCTGTG
GATGCAGGAGGGGTGCGCAAAGAATTTTTCTTGCTCATCATGAGGGAATT
ATTGGATCCTAAATACGGCATGTTTAGGTATTATGAAGATTCCAGGCTCA
TTTGGTTTTCTGATAAGACATTTGAAGACAGTGATTTGTTCCATTTGATT
GGTGTTATCTGTGGCTTAGCAATTTATAATTGTACCATTGTGGACCTCCA
TTTTCCTTTGGCTTTATATAAGAAACTACTGAAAAAGAAGCCATCCTTGG
ATGATTTGAAAGAACTAATGCCTGATGTTGGGAGAAGCATGCAACAGTTA
CTGGATTATCCAGAAGATGACATAGAGGAAACATTTTGTCTTAATTTTAC
GATCACAGTTGAAAACTTTGGTGCAACAGAAGTGAAAGAGCTGGTTCTAA
ATGGTGCAGACACAGCTGTTAACAAACAAAATCGGCAAGAGTTTGTCGAT
GCTTATGTGGATTACATATTCAATAAATCAGTGGCTTCCTTATTTGATGC
TTTTCATGCGGGCTTTCATAAGGTCTGTGGAGGAAAAGTCCTTCTGCTCT
TTCAGCCTAATGAACTACAAGCAATGGTCATTGGAAATACAAATTATGAT
TGGAAGGAACTGGAAAAGAATACAGAATACAAAGGGGAATATTGGGCAGA
ACATCCTACGATAAAAATTTTTTGGGAAGTATTTCACGAATTACCATTGG
AAAAGAAGAAACAGTTTCTGTTATTTTTGACAGGTAGTGATCGCATTCCT
ATTCTTGGTATGAAGAGTCTGAAACTAGTCATCCAGTCCACAGGAGGTGG
TGAGGAGTATCTCCCAGTTTCCCATACTTGTTTTAATCTTCTGGATCTTC
CAAAATATACAGAAAAAGAAACTCTACGCTCTAAACTGATCCAAGCTATT
GATCACAATGAAGGCTTCAGTTTAATATAAgcggccgc

Amino Acid Sequence

MYPYDVPDYAGSPGEFMLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINK
RVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQ
NIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPRNIKSL
SDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLL
KSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPN
LLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEIN
PRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSN
RKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNC
GPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSG
CLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAA
SLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALV
NLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERR
IFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINW
VQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQM
AIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDY
KKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWF
SDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDL
KELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGA
DTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQP
NELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKK
KQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKY
TEKETLRSKLIQAIDHNEGFSLI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65306
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Isoform a. Silent changes at Ser337 (AGC > AGT) compared to NCBI ref WT; created from Isoform b by insertion of D643-E650 ; Parental 41456
• Low Temp: No