cDNA Clone LRRK1

• Synonyms: OSMD; RIPK6; Roco1
• Plasmid: pcDNA5 FRT/TO GFP LRRK1 K651A Y971F
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP LRRK1 K651A Y971F
• Genbank: NM_024652
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGCTGGCATGTCGCAAAGACCCCCCAGCATGTACTGGTGTGTGGGGCCGGAGGAGTCAGCTGTGTGTCCAGAACGTGCCATGGAGACGCTTAACGGTGCCGGGGACACGGGCGGCAAGCCGTCCACGCGGGGCGGTGACCCTGCAGCGCGGTCCCGCAGGACGGAAGGCATCCGCGCCGCGTACAGGCGGGGAGACCGCGGCGGCGCCCGGGACCTGCTGGAGGAGGCCTGCGACCAGTGCGCGTCCCAGCTGGAAAAGGGCCAGCTTCTGAGCATCCCGGCAGCCTATGGGGATCTGGAGATGGTCCGCTACCTACTCAGCAAGAGACTGGTGGAGCTGCCCACCGAGCCCACGGATGACAACCCAGCCGTGGTGGCAGCGTATTTTGGACACACGGCAGTTGTGCAGGAATTGCTTGAGTCCTTACCAGGTCCCTGCAGTCCCCAGCGGCTTCTGAACTGGATGCTGGCCTTGGCTTGCCAGCGAGGGCACCTGGGGGTTGTGAAGCTCCTGGTCCTGACGCACGGGGCTGACCCGGAGAGCTACGCTGTCAGGAAGAATGAGTTCCCTGTCATCGTGCGCTTGCCCCTGTATGCGGCCATCAAGTCAGGGAATGAAGACATTGCAATATTCCTGCTTCGGCATGGGGCCTATTTCTGTTCCTACATCTTGCTGGATAGTCCTGACCCCAGCAAACATCTGCTGAGAAAGTACTTCATTGAAGCCAGTCCCTTGCCCAGCAGTTATCCGGGAAAAACAGCTCTCCGTGTGAAATGGTCCCATCTCAGACTGCCCTGGGTAGACCTAGACTGGCTCATAGACATCTCCTGCCAGATCACGGAGCTCGACCTTTCTGCCAACTGCCTGGCGACCCTCCCCTCGGTTATCCCCTGGGGCCTCATCAATCTCCGGAAGCTGAACCTCTCCGACAACCACCTGGGGGAGCTGCCTGGCGTGCAGTCATCGGACGAAATCATCTGTTCCAGGCTACTTGAAATTGACATTTCCAGCAACAAGTTGTCCCACCTCCCTCCTGGATTCTTGCACCTCTCAAAACTTCAAAAACTGACAGCTTCAAAAAATTGTTTAGAAAAATTGTTCGAAGAAGAAAATGCCACTAACTGGATAGGTTTACGGAAGCTACAGGAACTTGATATATCTGACAATAAATTGACAGAACTCCCTGCCCTGTTCCTTCACTCTTTCAAGTCCCTCAATTCTCTGAATGTCTCCAGAAACAACCTGAAGGTGTTTCCAGATCCCTGGGCCTGCCCTTTGAAATGTTGTAAAGCTTCCAGAAATGCCCTGGAATGTCTGCCAGACAAAATGGCTGTCTTTTGGAAAAATCACCTGAAGGATGTGGATTTCTCAGAAAACGCACTCAAAGAAGTTCCCCTGGGACTTTTCCAGCTTGATGCCCTCATGTTCTTGAGGTTACAGGGGAACCAGCTGGCGGCACTTCCACCTCAAGAGAAGTGGACCTGCAGGCAGCTCAAAACCCTGGATCTCTCCAGAAACCAACTTGGCAAAAATGAAGATGGACTGAAAACGAAGCGTATTGCCTTTTTCACCACCAGAGGTCGCCAGCGCTCCGGGACTGAGGCAGCAAGTGTGCTGGAATTTCCGGCCTTCCTAAGTGAGTCTTTGGAAGTCCTTTGCCTGAACGACAACCACCTCGACACAGTCCCTCCCTCGGTTTGCCTACTGAAGAGCTTATCAGAGCTCTACTTGGGAAACAACCCTGGCCTCCGGGAGCTCCCTCCTGAGCTGGGGCAGCTGGGCAACCTCTGGCAGCTGGACACTGAAGACCTGACCATCAGCAATGTGCCTGCAGAAATCCAAAAAGAAGGCCCCAAAGCAATGCTGTCTTACCTGCGTGCTCAGCTGCGGAAAGCGGAAAAGTGCAAGCTGATGAAGATGATCATCGTGGGTCCCCCGCGCCAGGGCGCGTCCACCCTCCTGGAGATCTTACAGACGGGGAGGGCCCCCCAGGTGGTGCATGGAGAGGCCACCATCAGGACCACCAAGTGGGAGCTCCAGAGGCCGGCTGGCTCAAGAGCCAAGGTTGAGTCCGTGGAGTTCAACGTCTGGGACATCGGGGGACCGGCCAGCATGGCCACTGTCAACCAGTGCTTCTTCACGGACAAGGCCCTGTACGTGGTGGTCTGGAACCTGGCGCTGGGGGAGGAGGCCGTGGCCAACCTCCAGTTCTGGCTGCTCAACATCGAGGCCAAGGCCCCAAACGCCGTGGTGCTGGTGGTCGGGACGCACCTGGATTTAATTGAAGCCAAGTTCCGTGTGGAAAGGATTGCAACGCTGCGTGCCTATGTGCTGGCACTCTGCCGCTCCCCCTCCGGCTCCAGGGCCACAGGCTTCCCAGACATCACCTTCAAACACTTACATGAGATTTCCTGCAAGAGCCTGGAAGGTCAGGAAGGGCTGCGACAGCTGATTTTCCACGTCACGTGCAGCATGAAGGACGTGGGCAGCACCATCGGCTGCCAGCGACTGGCAGGGCGGCTGATCCCCAGGAGCTACCTGAGCCTGCAGGAGGCCGTGCTGGCAGAGCAGCAGCGCCGCAGCCGGGACGACGACGTGCAGTACCTGACGGACAGGCAGCTGGAGCAGCTGGTGGAGCAGACGCCCGACAACGACATCAAGGACTACGAGGACCTGCAGTCAGCCATCAGCTTCCTCATAGAAACCGGCACCCTGCTCCATTTCCCGGACACCAGCCACGGCCTGAGGAACCTCTACTTCCTCGACCCTATTTGGCTCTCCGAATGTCTGCAGAGGATCTTTAATATTAAGGGCTCTCGGTCAGTGGCCAAGAATGGGGTGATCAGAGCAGAAGACCTCAGGATGCTGCTGGTGGGGACTGGCTTCACGCAGCAGACGGAAGAGCAGTTCTTCCAGTTCCTGGCCAAGTTTGAGATCGCCCTGCCCGTCGCCAATGACAGCTACCTCCTGCCCCATCTCCTTCCATCTAAACCTGGCCTGGACACCCACGGTATGCGGCACCCCACAGCCAACACCATTCAGAGGGTATTTAAGATGAGCTTCGTTCCCGTTGGCTTCTGGCAAAGGTTTATAGCACGGATGCTGATCAGCCTGGCGGAGATGGACCTGCAGCTTTTTGAAAACAAGAAGAATACTAAAAGCAGGAACAGGAAAGTCACCATTTACAGTTTTACAGGAAACCAGAGAAATCGCTGTAGCACATTCAGAGTGAAAAGAAATCAGACCATCTATTGGCAGGAAGGGCTCCTGGTCACTTTTGATGGGGGCTACCTCAGTGTGGAATCTTCCGACGTGAACTGGAAAAAGAAGAAAAGCGGAGGAATGAAAATTGTTTGCCAATCAGAAGTGAGGGACTTCTCAGCCATGGCTTTCATCACGGACCACGTCAATTCCTTGATTGATCAGTGGTTTCCCGCCCTGACAGCCACAGAGAGCGACGGGACGCCACTCATGGAGCAGTACGTGCCCTGCCCGGTCTGCGAGACAGCCTGGGCCCAGCACACGGACCCCAGTGAGAAATCAGAGGATGTGCAGTACTTCGACATGGAAGACTGTGTCCTGACGGCCATCGAGCGGGACTTCATCTCCTGCCCCAGACACCCGGACCTCCCCGTGCCGCTGCAGGAGCTGGTCCCTGAACTGTTCATGACCGACTTCCCGGCCAGGCTCTTCCTGGAGAACAGCAAGCTGGAGCACAGCGAGGACGAGGGCAGCGTCCTGGGCCAGGGCGGCAGTGGCACCGTCATCTACCGGGCCCGGTACCAGGGCCAGCCTGTGGCCGTCAAGCGCTTCCACATCAAAAAATTCAAGAACTTTGCTAACGTACCGGCAGACACCATGCTGAGGCACCTGCGGGCCACCGATGCCATGAAGAACTTCTCCGAGTTCCGGCAGGAGGCCAGCATGCTGCACGCGCTGCAGCACCCCTGCATCGTGGCGCTCATCGGCATCAGCATCCACCCGCTCTGCTTCGCCCTGGAGCTCGCGCCGCTCAGCAGCCTCAACACCGTGCTGTCCGAGAACGCCAGAGATTCTTCCTTTATACCCCTGGGACACATGCTCACCCAAAAAATAGCCTACCAGATCGCCTCGGGCCTGGCCTACCTGCACAAGAAAAACATCATCTTCTGTGACCTGAAGTCGGACAACATTCTGGTGTGGTCCCTTGACGTCAAGGAGCACATCAACATCAAGCTATCTGACTACGGGATTTCGAGGCAGTCATTCCATGAGGGCGCCCTAGGCGTGGAGGGCACTCCTGGCTACCAGGCCCCAGAGATCAGGCCTCGCATTGTATATGATGAGAAGGTAGATATGTTCTCCTATGGAATGGTGCTCTACGAGTTGCTGTCAGGACAGCGCCCTGCACTGGGCCACCACCAGCTCCAGATTGCCAAGAAGCTGTCCAAGGGCATCCGCCCGGTTCTGGGGCAGCCGGAGGAAGTGCAGTTCCGGCGACTGCAGGCGCTCATGATGGAGTGCTGGGACACTAAGCCAGAGAAGCGACCGCTGGCCCTGTCGGTGGTGAGCCAGATGAAGGACCCGACTTTTGCCACCTTCATGTATGAACTGTGCTGTGGGAAGCAGACAGCCTTCTTCTCATCCCAGGGCCAGGAGTACACCGTGGTGTTTTGGGATGGAAAAGAGGAGTCCAGGAACTACACGGTGGTGAACACAGAGAAGGGCCTCATGGAGGTGCAGAGGATGTGCTGCCCTGGGATGAAGGTGAGCTGCCAGCTCCAGGTCCAGAGATCCCTGTGGACAGCCACCGAGGACCAGAAAATCTACATCTACACCCTCAAGGGCATGTGCCCCTTAAACACACCCCAACAGGCCTTGGATACTCCAGCTGTCGTCACCTGCTTCTTGGCCGTGCCTGTTATTAAAAAGAATTCCTACCTGGTCTTAGCGGGCCTCGCCGATGGGCTTGTGGCTGTGTTTCCCGTGGTGCGGGGCACCCCAAAGGACAGCTGCTCCTACCTGTGCTCACACACAGCCAACAGGTCCAAGTTCAGCATCGCGGATGAAGACGCACGGCAGAACCCCTACCCAGTGAAGGCCATGGAGGTGGTCAACAGCGGCTCTGAGGTCTGGTACAGCAATGGGCCGGGCCTCCTTGTCATCGACTGTGCCTCCCTGGAGATCTGCAGGCGGCTGGAGCCCTACATGGCCCCCTCCATGGTTACGTCAGTCGTGTGCAGCTCTGAGGGCAGAGGGGAGGAGGTCGTCTGGTGCCTGGATGACAAGGCCAACTCCTTGGTGATGTACCACTCCACCACCTACCAGCTGTGTGCCCGGTACTTCTGCGGGGTCCCCAGCCCCCTCAGGGACATGTTTCCCGTGCGGCCCTTGGACACGGAACCCCCGGCAGCCAGCCACACGGCCAACCCAAAGGTGCCTGAGGGGGACTCCATCGCGGACGTGAGCATCATGTACAGTGAGGAGCTGGGCACGCAGATCCTGATCCACCAGGAATCACTCACTGACTACTGCTCCATGTCCTCCTACTCCTCATCCCCACCCCGCCAGGCTGCCAGGTCCCCCTCAAGCCTCCCCAGCTCCCCAGCAAGTTCTTCCAGTGTGCCTTTCTCCACCGACTGCGAGGACTCAGACATGCTACATACGCCCGGTGCTGCCTCCGACAGGTCTGAGCATGACCTGACCCCCATGGACGGGGAGACCTTCAGCCAGCACCTGCAGGCCGTGAAGATCCTCGCCGTCAGAGACCTCATTTGGGTCCCCAGGCGCGGTGGAGATGTTATCGTCATTGGCCTGGAGAAGGATTCTGGCGCCCAGCGGGGCCGAGTCATTGCCGTCTTAAAAGCCCGAGAGCTGACTCCGCATGGGGTGCTGGTGGATGCTGCCGTGGTGGCAAAGGACACTGTTGTGTGCACCTTTGAAAATGAAAACACAGAGTGGTGCCTGGCCGTCTGGAGGGGCTGGGGCGCCAGGGAGTTCGACATTTTCTACCAGTCCTACGAGGAGCTGGGCCGGCTGGAGGCTTGCACTCGCAAGAGAAGGTAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMAGMSQRPPSMYWCVGPEESAVCPERAMETLNGAGDTGGKPSTRGGDPAARSRRTE
GIRAAYRRGDRGGARDLLEEACDQCASQLEKGQLLSIPAAYGDLEMVRYLLSKRLVELPTEPTDDNPAVVAAYFGHTAVVQELLESLPGPCSPQRLLNWM
LALACQRGHLGVVKLLVLTHGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIAIFLLRHGAYFCSYILLDSPDPSKHLLRKYFIEASPLPSSYPGKTAL
RVKWSHLRLPWVDLDWLIDISCQITELDLSANCLATLPSVIPWGLINLRKLNLSDNHLGELPGVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQK
LTASKNCLEKLFEEENATNWIGLRKLQELDISDNKLTELPALFLHSFKSLNSLNVSRNNLKVFPDPWACPLKCCKASRNALECLPDKMAVFWKNHLKDVD
FSENALKEVPLGLFQLDALMFLRLQGNQLAALPPQEKWTCRQLKTLDLSRNQLGKNEDGLKTKRIAFFTTRGRQRSGTEAASVLEFPAFLSESLEVLCLN
DNHLDTVPPSVCLLKSLSELYLGNNPGLRELPPELGQLGNLWQLDTEDLTISNVPAEIQKEGPKAMLSYLRAQLRKAEKCKLMKMIIVGPPRQGASTLLE
ILQTGRAPQVVHGEATIRTTKWELQRPAGSRAKVESVEFNVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVANLQFWLLNIEAKAPNAVVLVVG
THLDLIEAKFRVERIATLRAYVLALCRSPSGSRATGFPDITFKHLHEISCKSLEGQEGLRQLIFHVTCSMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLA
EQQRRSRDDDVQYLTDRQLEQLVEQTPDNDIKDYEDLQSAISFLIETGTLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRM
LLVGTGFTQQTEEQFFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENKKNTKSR
NRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGMKIVCQSEVRDFSAMAFITDHVNSLIDQWFPALTATESDGT
PLMEQYVPCPVCETAWAQHTDPSEKSEDVQYFDMEDCVLTAIERDFISCPRHPDLPVPLQELVPELFMTDFPARLFLENSKLEHSEDEGSVLGQGGSGTV
IYRARYQGQPVAVKRFHIKKFKNFANVPADTMLRHLRATDAMKNFSEFRQEASMLHALQHPCIVALIGISIHPLCFALELAPLSSLNTVLSENARDSSFI
PLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDVKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYE
LLSGQRPALGHHQLQIAKKLSKGIRPVLGQPEEVQFRRLQALMMECWDTKPEKRPLALSVVSQMKDPTFATFMYELCCGKQTAFFSSQGQEYTVVFWDGK
EESRNYTVVNTEKGLMEVQRMCCPGMKVSCQLQVQRSLWTATEDQKIYIYTLKGMCPLNTPQQALDTPAVVTCFLAVPVIKKNSYLVLAGLADGLVAVFP
VVRGTPKDSCSYLCSHTANRSKFSIADEDARQNPYPVKAMEVVNSGSEVWYSNGPGLLVIDCASLEICRRLEPYMAPSMVTSVVCSSEGRGEEVVWCLDD
KANSLVMYHSTTYQLCARYFCGVPSPLRDMFPVRPLDTEPPAASHTANPKVPEGDSIADVSIMYSEELGTQILIHQESLTDYCSMSSYSSSPPRQAARSP
SSLPSSPASSSSVPFSTDCEDSDMLHTPGAASDRSEHDLTPMDGETFSQHLQAVKILAVRDLIWVPRRGGDVIVIGLEKDSGAQRGRVIAVLKARELTPH
GVLVDAAVVAKDTVVCTFENENTEWCLAVWRGWGAREFDIFYQSYEELGRLEACTRKRR*

• Vector Type: Mammalian
• Full Snapgene Map: pcDNA5 FRT/TO GFP LRRK1 K651A Y971F1.58 MB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU61853
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All LRRK1 plasmids MUST be grown at 30C or less to prevent recombination.
• Low Temp: No