cDNA Clone Ubiquitin

• Synonyms: x
• Plasmid: pHISTEV30a Ubiquitin KallR M-2C
• Parent Plasmid: pHisTEV30a
• Expressed: 6HIS TEV Ubiquitin ( K6R, K11R, K27R, K29R, K33R, K48R, K63R) M-2C
• Genbank: 7316
• Tag: 6His
• Species: Human

Sequence of Insert

see attached Snapgene file

Amino Acid Sequence

see attached Snapgene file

• Vector Type: Bacterial
• Antibiotic: Kan
• Full Snapgene Map: pHISTEV30a Ubiquitin KallR M-2C99.58 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU71379
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: differs from NCBI reference sequence in having a silent mutation at Arg73 (a220c). Clone contains Cys residue two amino acids upstream of Ubiquitin start -introduced for labelling of ubiquitin with fluorescent dye; Excised KallR ubiquitin from 20052 BglII (internal native site at Ile3) Not and replaced Bgl/Not fragment in 29939 to give KallR version of 29939 WT
• Low Temp: No