cDNA Clone
Ubiquitin

Synonyms
x
Plasmid
pHISTEV30a Ubiquitin KallA M-2C
Parent Plasmid
pHisTEV30a
Expressed
6HIS TEV Ubiquitin ( K6A, K11A, K27A, K29A, K33A, K48A, K63A) M-2C
Genbank
7316
Tag
6His
Species
Human
Sequence of Insert
see attached Snapgene file
Amino Acid Sequence
see attached Snapgene file
Vector Type
Bacterial
Antibiotic
Kan
Full Snapgene Map
pHISTEV30a Ubiquitin KallA M-2C147.83 KB Snapgene files can be opened using software available here: www.snapgene.com
DU Number
DU71412
Location
PPU
MTA
Notes
differs from NCBI reference sequence in having a silent mutation at Arg73 (a220c). Clone contains Cys residue two amino acids upstream of Ubiquitin start -introduced for labelling of ubiquitin with fluorescent dye
BglII-Not flanked GeneArt string (using internal native site at Ile3) used to replace Bgl/Not fragment in 29939 to give KallA version of 29939 WT
Low Temp
No
Price
£125.00
To submit an order for this reagent acceptance of the Dundee Material Transfer Agreement is required at checkout.