cDNA Clone PTEN

• Plasmid: pFBHTb PTEN
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-PTEN
• Genbank: CR450306
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGACAGCCATCATCAAAGAGATCGTTAGCAGAAACAAAAGGAG
ATATCAAGAGGATGGATTCGACTTAGACTTGACCTATATTTATCCAAACA
TTATTGCTATGGGATTTCCTGCAGAAAGACTTGAAGGCGTATACAGGAAC
AATATTGATGATGTAGTAAGGTTTTTGGATTCAAAGCATAAAAACCATTA
CAAGATATACAATCTTTGTGCTGAAAGACATTATGACACCGCCAAATTTA
ATTGCAGAGTTGCACAATATCCTTTTGAAGACCATAACCCACCACAGCTA
GAACTTATCAAACCCTTTTGTGAAGATCTTGACCAATGGCTAAGTGAAGA
TGACAATCATGTTGCAGCAATTCACTGTAAAGCTGGAAAGGGACGAACTG
GTGTAATGATATGTGCATATTTATTACATCGGGGCAAATTTTTAAAGGCA
CAAGAGGCCCTAGATTTCTATGGGGAAGTAAGGACCAGAGACAAAAAGGG
AGTAACTATTCCCAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGT
TAAAGAATCATCTGGATTATAGACCAGTGGCACTGTTGTTTCACAAGATG
ATGTTTGAAACTATTCCAATGTTCAGTGGCGGAACTTGCAATCCTCAGTT
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA
CACGACGGGAAGACAAGTTCATGTACTTTGAGTTCCCTCAGCCGTTACCT
GTGTGTGGTGATATCAAAGTAGAGTTCTTCCACAAACAGAACAAGATGCT
AAAAAAGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAG
GACCAGAGGAAACCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAA
GAAATCGATAGCATTTGCAGTATAGAGCGTGCAGATAATGACAAGGAATA
TCTAGTACTTACTTTAACAAAAAATGATCTTGACAAAGCAAATAAAGACA
AAGCCAACCGATACTTTTCTCCAAATTTTAAGGTGAAGCTGTACTTCACA
AAAACAGTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGT
AACACCAGATGTTAGTGACAACGAACCTGATCATTATAGATATTCTGACA
CCACTGACTCTGATCCAGAGAATGAACCTTTTGATGAAGATCAGCATACA
CAAATTACAAAAGTCTGAGGGATTC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLNFQGAMGSMTAIIKEIVSRNKRRYQEDGFD
LDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCA
ERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAI
HCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQR
RYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKV
KIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFH
FWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTK
NDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDN
EPDHYRYSDTTDSDPENEPFDEDQHTQITKV*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU3931
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6 His for baculovirus expression.; Calc Mol Weight : 50456 Daltons