cDNA Clone MKK5

• Plasmid: pGEX4T-1-MKK5
• Parent Plasmid: pGEX4T1
• Expressed: GST-MKK5
• Genbank: AAH08838
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCTGTGGCTAGCCCTTGGCCCCTTTCCTGCCATGGAGAACCA
GGTGCTGGTAATTCGCATCAAGATCCCAAATAGTGGCGCGGTGGACTGGA
CAGTGCACTCCGGGCCGCAGTTACTCTTCAGGGATGTGCTGGATGTGATA
GGCCAGGTTCTGCCTGAAGCAACAACTACAGCATTTGAATATGAAGATGA
AGATGGTGATCGAATTACAGTGAGAAGTGATGAGGAAATGAAGGCAATGC
TGTCATATTATTATTCCACAGTAATGGAACAGCAAGTAAATGGACAGTTA
ATAGAGCCTCTGCAGATATTTCCAAGAGCCTGCAAGCCTCCTGGGGAACG
GAACATACATGGCCTGAAGGTGAATACTCGGGCCGGACCCTCTCAACACA
GCAGCCCAGCAGTCTCAGATTCACTTCCAAGCAATAGCTTAAAGAAGTCT
TCTGCTGAACTGAAAAAAATACTAGCCAATGGCCAGATGAATGAACAAGA
CATACGATATCGGGACACTCTTGGTCATGGCAACGGAGGCACAGTCTACA
AAGCATATCATGTCCCGAGTGGGAAAATATTAGCTGTAAAGGTCATACTA
CTAGATATTACACTGGAACTTCAGAAGCAAATTATGTCTGAATTGGAAAT
TCTTTATAAGTGCGATTCATCATATATCATTGGATTTTATGGAGCATTTT
TTGTAGAAAACAGGATTTCAATATGTACAGAATTCATGGATGGGGGATCT
TTGGATGTATATAGGAAAATGCCAGAACATGTCCTTGGAAGAATTGCAGT
AGCAGTTGTTAAAGGCCTTACTTATTTGTGGAGTTTAAAGATTTTACATA
GAGACGTGAAGCCCTCCAATATGCTAGTAAACACAAGAGGACAGGTTAAG
CTGTGTGATTTTGGAGTTAGCACTCAGCTGGTGAATTCTATAGCCAAGAC
GTATGTTGGAACAAATGCTTATATGGCGCCTGAAAGGATTTCAGGGGAGC
AGTATGGAATTCATTCTGATGTCTGGAGCTTAGGAATCTCTTTTATGGAG
CTTGCTCTTGGGAGGTTTCCATATCCTCAGATTCAGAAAAACCAGGGATC
TTTAATGCCTCTCCAGCTTCTGCAGTGCATTGTTGATGAGGATTCGCCCG
TCCTTCCAGTTGGAGAGTTCTCGGAGCCATTTGTACATTTCATCACTCAG
TGTATGCGAAAACAGCCAAAAGAAAGGCCAGCACCTGAAGAATTGATGGG
CCACCCGTTCATCGTGCAGTTCAATGATGGAAATGCCGCCGTGGTGTCCA
TGTGGGTGTGCCGGGCGCTGGAGGAGAGGCGGAGCCAGCAGGGGCCCCCG
TGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSMLWLALGPFPAMENQVLVIRIKIP
NSGAVDWTVHSGPQLLFRDVLDVIGQVLPEATTTAFEYEDEDGDRITVRS
DEEMKAMLSYYYSTVMEQQVNGQLIEPLQIFPRACKPPGERNIHGLKVNT
RAGPSQHSSPAVSDSLPSNSLKKSSAELKKILANGQMNEQDIRYRDTLGH
GNGGTVYKAYHVPSGKILAVKVILLDITLELQKQIMSELEILYKCDSSYI
IGFYGAFFVENRISICTEFMDGGSLDVYRKMPEHVLGRIAVAVVKGLTYL
WSLKILHRDVKPSNMLVNTRGQVKLCDFGVSTQLVNSIAKTYVGTNAYMA
PERISGEQYGIHSDVWSLGISFMELALGRFPYPQIQKNQGSLMPLQLLQC
IVDEDSPVLPVGEFSEPFVHFITQCMRKQPKERPAPEELMGHPFIVQFND
GNAAVVSMWVCRALEERRSQQGPP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3779
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.; Calc Mol Weight : 76354 Daltons