cDNA Clone DAZAP1-b

• Plasmid: pGEX6P-1-6His DAZAP1-b 155-407 T269D T315D
• Parent Plasmid: pGEX6P1
• Expressed: GST-6His DAZAP1-b 155-407 T269D T315D
• Genbank: NM_018959
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCATCATCACCATCACCATGGTTTTGGATTTATTACTTTCGAGGA
CGAACAATCAGTGGACCAGGCTGTCAACATGCATTTTCACGACATCATGG
GCAAAAAAGTGGAAGTTAAACGAGCTGAGCCTCGGGACAGCAAGAGCCAA
GCGCCGGGACAGCCAGGTGCCAGCCAGTGGGGGAGCCGGGTTGTGCCCAA
CGCTGCCAATGGCTGGGCAGGCCAGCCCCCGCCCACGTGGCAGCAAGGAT
ATGGCCCGCAAGGAATGTGGGTGCCGGCAGGACAGGCGATTGGTGGCTAT
GGACCGCCCCCTGCAGGAAGAGGAGCCCCCCCGCCACCCCCACCGTTCAC
CTCCTACATCGTGTCCACCCCTCCTGGAGGCTTTCCCCCTCCCCAGGGCT
TCCCTCAGGGCTACGGTGCCCCGCCACAGTTCAGTTTTGGCTACGGGCCT
CCACCTCCACCGCCAGATCAGTTTGCCCCTCCGGGGGTTCCTCCTCCACC
AGCCACTCCCGGGGCAGCACCTCTGGCTTTCCCACCGCCTCCGTCTCAGG
CTGCCCCGGACATGAGCAAGCCCCCGACAGCTCAGCCAGACTTCCCCTAT
GGTCAGTATGCAGGTTACGGGCAGGACTTGAGTGGCTTCGGACAGGGCTT
CTCAGACCCCAGCCAGCAGCCTCCTTCCTACGGGGGTCCCTCCGTGCCAG
GGTCGGGGGGCCCCCCCGCCGGCGGCAGCGGCTTTGGACGAGGGCAGAAC
CACAACGTGCAAGGGTTCCACCCCTACCGACGCTAGGGATCCGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSHHHHHHGFGFITFEDEQSV
DQAVNMHFHDIMGKKVEVKRAEPRDSKSQAPGQPGASQWGSRVVPNAANG
WAGQPPPTWQQGYGPQGMWVPAGQAIGGYGPPPAGRGAPPPPPPFTSYIV
SDPPGGFPPPQGFPQGYGAPPQFSFGYGPPPPPPDQFAPPGVPPPPADPG
AAPLAFPPPPSQAAPDMSKPPTAQPDFPYGQYAGYGQDLSGFGQGFSDPS
QQPPSYGGPSVPGSGGPPAGGSGFGRGQNHNVQGFHPYRR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3775
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST/6His tag for bacterial expression.; Calc Mol Weight : 53676 Daltons