cDNA Clone PINK1

• Plasmid: pGEX6P-1-PINK1 139-581
• Parent Plasmid: pGEX6P1
• Expressed: GST-PINK1 139-581
• Genbank: BC028215
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGGGCCTGACCCGTTGGACACGAGACGCTTGCAGGGCTTTCGGCT
GGAGGAGTATCTGATAGGGCAGTCCATTGGTAAGGGCTGCAGTGCTGCTG
TGTATGAAGCCACCATGCCTACATTGCCCCAGAACCTGGAGGTGACAAAG
AGCACCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAGGAGA
AGGGCAGGAGCGAGCTCCGGGGGCCCCTGCCTTCCCCTTGGCCATCAAGA
TGATGTGGAACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAACACA
ATGAGCCAGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGGAGTA
TGGAGCAGTCACTTACAGAAAATCCAAGAGAGGTCCCAAGCAACTAGCCC
CTCACCCCAACATCATCCGGGTTCTCCGCGCCTTCACCTCTTCCGTGCCG
CTGCTGCCAGGGGCCCTGGTCGACTACCCTGATGTGCTGCCCTCACGCCT
CCACCCTGAAGGCCTGGGCCATGGCCGGACGCTGTTCCTCGTTATGAAGA
ACTATCCCTGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAGCCCC
CGCCTCGCCGCCATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACCATCT
GGTTCAACAGGGCATCGCGCACAGAGACCTGAAATCCGACAACATCCTTG
TGGAGCTGGACCCAGACGGCTGCCCCTGGCTGGTGATCGCAGATTTTGGC
TGCTGCCTGGCTGATGAGAGCATCGGCCTGCAGTTGCCCTTCAGCAGCTG
GTACGTGGATCGGGGCGGAAACGGCTGTCTGATGGCCCCAGAGGTGTCCA
CGGCCCGTCCTGGCCCCAGGGCAGTGATTGACTACAGCAAGGCTGATGCC
TGGGCAGTGGGAGCCATCGCCTATGAAATCTTCGGGCTTGTCAATCCCTT
CTACGGCCAGGGCAAGGCCCACCTTGAAAGCCGCAGCTACCAAGAGGCTC
AGCTACCTGCACTGCCCGAGTCAGTGCCTCCAGACGTGAGACAGTTGGTG
AGGGCACTGCTCCAGCGAGAGGCCAGCAAGAGACCATCTGCCCGAGTAGC
CGCAAATGTGCTTCATCTAAGCCTCTGGGGTGAACATATTCTAGCCCTGA
AGAATCTGAAGTTAGACAAGATGGTTGGCTGGCTCCTCCAACAATCGGCC
GCCACTTTGTTGGCCAACAGGCTCACAGAGAAGTGTTGTGTGGAAACAAA
AATGAAGATGCTCTTTCTGGCTAACCTGGAGTGTGAAACGCTCTGCCAGG
CAGCCCTCCTCCTCTGCTCATGGAGGGCAGCCCTGTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSGPDPLDTRRLQGFRLEEYL
IGQSIGKGCSAAVYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQER
APGAPAFPLAIKMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVT
YRKSKRGPKQLAPHPNIIRVLRAFTSSVPLLPGALVDYPDVLPSRLHPEG
LGHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQG
IAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDR
GGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQG
KAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVL
HLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKML
FLANLECETLCQAALLLCSWRAAL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3705
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.