cDNA Clone PINK1

• Plasmid: pEBG6P-PINK1 1573-1574 ins
• Parent Plasmid: pEBG6P
• Expressed: GST-PINK1 1573-1574 ins
• Genbank: BC028215
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCGGTGCGACAGGCGCTGGGCCGCGGCCTGCAGCTGGGTCGAGC
GCTGCTGCTGCGCTTCACGGGCAAGCCCGGCCGGGCCTACGGCTTGGGGC
GGCCGGGCCCGGCGGCGGGCTGTGTCCGCGGGGAGCGTCCAGGCTGGGCC
GCAGGACCGGGCGCGGAGCCTCGCAGGGTCGGGCTCGGGCTCCCTAACCG
TCTCCGCTTCTTCCGCCAGTCGGTGGCCGGGCTGGCGGCGCGGTTGCAGC
GGCAGTTCGTGGTGCGGGCCTGGGGCTGCGCGGGCCCTTGCGGCCGGGCA
GTCTTTCTGGCCTTCGGGCTAGGGCTGGGCCTCATCGAGGAAAAACAGGC
GGAGAGCCGGCGGGCGGTCTCGGCCTGTCAGGAGATCCAGGCAATTTTTA
CCCAGAAAAGCAAGCCGGGGCCTGACCCGTTGGACACGAGACGCTTGCAG
GGCTTTCGGCTGGAGGAGTATCTGATAGGGCAGTCCATTGGTAAGGGCTG
CAGTGCTGCTGTGTATGAAGCCACCATGCCTACATTGCCCCAGAACCTGG
AGGTGACAAAGAGCACCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCAGT
GCACCAGGAGAAGGGCAGGAGCGAGCTGCGGGGGCCCCTGCCTTCCCCTT
GGCCATCAAGATGATGTGGAACATCTCGGCAGGTTCCTCCAGCGAAGCCA
TCTTGAACACAATGAGCCAGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTG
GCTGGGGAGTATGGAGCAGTCACTTACAGAAAATCCAAGAGAGGTCCCAA
GCAACTAGCCCCTCACCCCAACATCATCCGGGTTCTCCGCGCCTTCACCT
CTTCCGTGCCGCTGCTGCCAGGGGCCCTGGTCGACTACCCTGATGTGCTG
CCCTCACGCCTCCACCCTGAAGGCCTGGGCCATGGCCGGACGCTGTTCCT
CGTTATGAAGAACTATCCCTGTACCCTGCGCCAGTACCTTTGTGTGAACA
CACCCAGCCCCCGCCTCGCCGCCATGATGCTGCTGCAGCTGCTGGAAGGC
GTGGACCATCTGGTTCAACAGGGCATCGCGCACAGAGACCTGAAATCCGA
CAACATCCTTGTGGAGCTGGACCCAGACGGCTGCCCCTGGCTGGTGATCG
CAGATTTTGGCTGCTGCCTGGCTGATGAGAGCATCGGCCTGCAGTTGCCC
TTCAGCAGCTGGTACGTGGATCGGGGCGGAAACGGCTGTCTGATGGCCCC
AGAGGTGTCCACGGCCCGTCCTGGCCCCAGGGCAGTGATTGACTACAGCA
AGGCTGATGCCTGGGCAGTGGGAGCCATCGCCTATGAAATCTTCGGGCTT
GTCAATCCCTTCTACGGCCAGGGCAAGGCCCACCTTGAAAGCCGCAGCTA
CCAAGAGGCTCAGCTACCTGCACTGCCCGAGTCAGTGCCTCCAGACGTGA
GACAGTTGGTGAGGGCACTGCTCCAGCGAGAGGCCAGCAAGAGACCATCT
GCCCGAGTAGCCGCAAATGTGCTTCATCTAAGCCTCTGGGGTGAACATAT
TCTAGCCCTGAAGAATCTGAAGTTATTAGGACAAGATGGTTGGCTGGCTC
CTCCAACAATCGGCCGCCACTTTGTTGGCCAACAGGCTCACAGAGAAGTG
TTGTGTGGAAACAAAAATGAAGATGCTCTTTCTGGCTAACCTGGAGTGTG
AAACGCTCTGCCAGGCAGCCCTCCTCCTCTGCTCATGGAGGGCAGCCCTG
TGAGCGGCCGCGCAAGGGCGAATTCCAGCACACTGGCGGCCGTTACTAGT
GGATCC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSAVRQALGRGLQLG
RALLLRFTGKPGRAYGLGRPGPAAGCVRGERPGWAAGPGAEPRRVGLGLP
NRLRFFRQSVAGLAARLQRQFVVRAWGCAGPCGRAVFLAFGLGLGLIEEK
QAESRRAVSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGK
GCSAAVYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERAAGAPAF
PLAIKMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRG
PKQLAPHPNIIRVLRAFTSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTL
FLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLK
SDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLM
APEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESR
SYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGE
HILALKNLKLLGQDGWLAPPTIGRHFVGQQAHREVLCGNKNEDALSG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3703
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression. Insertion causes frame change after aa 524 leading to truncation.