cDNA Clone p38 alpha MAPK (rat)

• Plasmid: pGEX6P-1-SAPK2a RAT
• Parent Plasmid: pGEX6P1
• Expressed: GST-SAPK2a rat
• Genbank: RNU73142
• Tag: GST
• Species: Rat

Sequence of Insert

GGATCCATGTCGCAGGAAAGGCCCACGTTCTACCGGCAGGAGCTGAACAA
GACCGTCTGGGAGGTGCCCGAGCGATACCAGAACCTGTCCCCGGTGGGCT
CGGGAGCCTACGGCTCGGTGTGTGCTGCTTTTGATACAAAGACGGGACAT
CGTGTGGCAGTGAAGAAGCTGTCGAGACCGTTTCAGTCCATCATTCACGC
CAAAAGGACCTACAGGGAGCTGCGGCTGCTGAAGCACATGAAGCACGAGA
ATGTGATTGGTCTGTTGGATGTGTTTACACCTGCAAGGTCCCTGGAGGAA
TTCAACGATGTGTACCTGGTGACCCATCTCATGGGGGCAGACCTGAACAA
CATCGTGAAGTGTCAGAAGCTTACCGATGACCACGTTCAGTTTCTTATCT
ACCAGATCCTGCGAGGGCTGAAGTATATACACTCGGCTGACATAATCCAC
AGGGACCTAAAGCCCAGCAACCTCGCTGTGAATGAAGACTGTGAGCTGAA
GATTCTGGATTTTGGGCTGGCTCGGCACACTGATGACGAAATGACCGGCT
ACGTGGCTACCCGGTGGTACAGAGCCCCCGAGATTATGCTGAATTGGATG
CACTACAACCAGACAGTGGATATTTGGTCCGTGGGCTGCATCATGGCTGA
GCTGTTGACCGGAAGAACGTTGTTTCCTGGTACAGACCATATTGATCAGT
TGAAGCTCATTTTAAGACTCGTTGGAACCCCAGGGGCTGAGCTTCTGAAG
AAAATCTCCTCAGAGTCTGCAAGAAACTACATTCAGTCTCTGGCCCAGAT
GCCGAAGATGAACTTCGCAAATGTATTTATTGGTGCCAATCCCCTGGCTG
TCGACCTGCTGGAAAAGATGCTGGTTTTGGACTCAGATAAGAGGATCACA
GCAGCCCAAGCTCTTGCGCATGCCTACTTTGCTCAGTACCACGACCCTGA
TGATGAGCCAGTGGCTGACCCTTATGACCAGTCCTTTGAAAGCAGGGACC
TCCTTATAGACGAATGGAAGAGCCTGACCTACGATGAAGTCATTAGCTTT
GTGCCACCGCCCCTTGACCAAGAAGAAATGGAGTCCTGAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSQERPTFYRQELNKTVWE
VPERYQNLSPVGSGAYGSVCAAFDTKTGHRVAVKKLSRPFQSIIHAKRTY
RELRLLKHMKHENVIGLLDVFTPARSLEEFNDVYLVTHLMGADLNNIVKC
QKLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNEDCELKILDF
GLARHTDDEMTGYVATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLTG
RTLFPGTDHIDQLKLILRLVGTPGAELLKKISSESARNYIQSLAQMPKMN
FANVFIGANPLAVDLLEKMLVLDSDKRITAAQALAHAYFAQYHDPDDEPV
ADPYDQSFESRDLLIDEWKSLTYDEVISFVPPPLDQEEMES*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3649
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression. There are 2 published protein sequences for rat SAPK2a, the above differs to the genbank reference at E321 and DF332L. The cloned sequence with produced by RT and agrees with the genomic sequence.