cDNA Clone PDE3A

• Plasmid: pEGFP-C3-PDE3A
• Parent Plasmid: pEGFPC3
• Expressed: GFP-PDE3A
• Genbank: CAA06304.1 GI:3059109
• Tag: GFP
• Species: Human

Sequence of Insert

GAATTCGCCCTTCGGGTACCATCGCCTTGACTAGCGCGGTCAGGACCGTG
TCCCTCATTTCCTTAGAGAGGTTCAAGGTCGCCTGGAGACCTTACCTGGC
GTACCTGGCCGGCGTGCTGGGGATCCTCTTGGCCAGGTACGTGGAACAAA
TCTTGCCGCAGTCCGCGGAGGCGGCTCCAAGGGAGCATTTGGGGTCCCAG
CTGATTGCTGGGACCAAGGAAGATATCCCGGTGTTTAAGAGGAGGAGGCG
GTCCAGCTCCGTCGTGTCCGCCGAGATGTCCGGCTGCAGCAGCAAGTCCC
ATCGGAGGACCTCCCTGCCCTGTATACCGAGGGAACAGCTCATGGGGCAT
TCAGAATGGGACCACAAACGAGGGCCAAGAGGATCACAGTCTTCAGGAAC
CAGTATTACTGTGGACATCGCCGTCATGGGCGAAGCCACGGCCTCATTAC
CGACCTCCTGGCAGACCCTTCTCTTCCACCAAACGTGTGCCACATCCTTG
AGAGCCGTGAGCAACTTGCTCAGCACACAGCTCACCTTCCAGGCCATTCA
CAAGCCCAGAGTGAATCCCGTTACTTCGCTCAGTGAAAACTATACCTGTT
CTGACTCTGAAGAGAGCTCTGAAAAAGACAAGCTTGCTATTCCAAAGCGC
CTGAGAAGGAGTTTGCCTCCTGGCTTGTTGAGACGAGTTTCTTCCACTTG
GACCACCACCACCTCGGCCACAGGTCTACCCACCTTGGAGCCTGCACCAG
TACGGAGAGACCGCAGCACCAGCATCAAACTGCAGGAAGCACCTTCATCC
AGTCCTGATTCTTGGAATAATCCAGTGATGATGACCCTCACCAAAAGCAG
ATCCTTTACTTCATCCTATGCTATTTCTGCAGCTAACCATGTAAAGGCTA
AAAAGCAAAGTCGACCAGGTGCCCTCGCTAAAATTTCACCTCTTTCATCG
CCCTGCTCCTCACCTCTCCAAGGGACTCCTGCCAGCAGCCTGGTCAGCAA
AATTTCTGCAGTGCAGTTTCCAGAATCTGCTGACACAACTGCCAAACAAA
GCCTAGGTTCTCACAGGGCCTTAACTTACACTCAGAGTGCCCCAGACCTA
TCCCCTCAAATCCTGACTCCACCTGTTATATGTAGCAGCTGTGGCAGACC
ATATTCCCAAGGGAATCCTGCTGATGAGCCCCTGGAGAGAAGTGGGGTAG
CCACTCGGACACCAAGTCGAACAGATGACACTGCTCAAGTTACCTCTGAT
TATGAAACCAATAACAACAGTGACAGCAGTGACATTGTACAGAATGAAGA
TGAAACAGAGTGCCTGAGAGAGCCTCTGAGGAAAGCATCGGCTTGCAGCA
CCTATGCTCCTGAGACCATGATGTTTCTGGACAAACCAATTCTTGCTCCC
GAACCTCTTGTCATGGATAACCTGGACTCAATTATGGAGCAGCTAAATAC
TTGGAATTTTCCAATTTTTGATTTAGTGGAAAATATAGGAAGAAAATGTG
GCCGTATTCTTAGTCAGGTATCTTACAGACTTTTTGAAGACATGGGCCTC
TTTGAAGCTTTTAAAATTCCAATTAGGGAATTTATGAATTATTTTCATGC
TTTGGAGATTGGATATAGGGATATTCCTTATCATAACAGAATCCATGCCA
CTGATGTTTTACATGCTGTTTGGTATCTTACTACACAGCCTATTCCAGGC
CTCTCAACTGTGATTAATGATCATGGTTCAACCAGTGATTCAGATTCTGA
CAGTGGATTTACACATGGACATATGGGATATGTATTCTCAAAAACGTATA
ATGTGACAGATGATAAATACGGATGTCTGTCTGGGAATATCCCTGCCTTG
GAGTTGATGGCGCTGTATGTGGCTGCAGCCATGCACGATTATGATCATCC
AGGAAGGACTAATGCTTTCCTGGTTGCAACTAGTGCTCCTCAGGCGGTGC
TATATAACGATCGTTCAGTTTTGGAGAATCATCACGCAGCTGCTGCATGG
AATCTTTTCATGTCCCGGCCAGAGTATAACTTCTTAATTAACCTTGACCA
TGTGGAATTTAAGCATTTCCGTTTCCTTGTCATTGAAGCAATTTTGGCCA
CTGACCTGAAGAAACACTTTGACTTCGTAGCCAAATTTAATGGCAAGGTA
AATGATGATGTTGGAATAGATTGGACCAATGAAAATGATCGTCTACTGGT
TTGTCAAATGTGTATAAAGTTGGCTGATATCAATGGTCCAGCTAAATGTA
AAGAACTCCATCTTCAGTGGACAGATGGTATTGTCAATGAATTTTATGAA
CAGGGTGATGAAGAGGCCAGCCTTGGATTACCCATAAGCCCCTTCATGGA
TCGTTCTGCTCCTCAGCTGGCCAACCTTCAGGAATCCTTCATCTCTCACA
TTGTGGGGCCTCTGTGCAACTCCTATGATTCAGCAGGACTAATGCCTGGA
AAATGGGTGGAAGACAGCGATGAGTCAGGAGATACTGATGACCCAGAAGA
AGAGGAGGAAGAAGCACCAGCACCAAATGAAGAGGAAACCTGTGAAAATA
ATGAATCTCCAAAAAAGAAGACTTTCAAAAGGAGAAAAATCTACTGCCAA
ATAACTCAGCACCTCTTACAGAACCACAAGATGTGGAAGAAAGTCATTGA
AGAGGAGCAACGGTTGGCAGGCATAGAAAATCAATCCCTGGACCAGACCC
CTCAGTCGCACTCTTCAGAACAGATCCAGGCTATCAAGGAAGAAGAAGAA
GAGAAAGGGAAACCAAGAGGCGAGGAGATACCAACCCAAAAGCCAGACCA
GTGAGCGGCCGCCGAAGGGCGAATTC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKYSDLELKLRIR
PSGTIALTSAVRTVSLISLERFKVAWRPYLAYLAGVLGILLARYVEQILP
QSAEAAPREHLGSQLIAGTKEDIPVFKRRRRSSSVVSAEMSGCSSKSHRR
TSLPCIPREQLMGHSEWDHKRGPRGSQSSGTSITVDIAVMGEATASLPTS
WQTLLFHQTCATSLRAVSNLLSTQLTFQAIHKPRVNPVTSLSENYTCSDS
EESSEKDKLAIPKRLRRSLPPGLLRRVSSTWTTTTSATGLPTLEPAPVRR
DRSTSIKLQEAPSSSPDSWNNPVMMTLTKSRSFTSSYAISAANHVKAKKQ
SRPGALAKISPLSSPCSSPLQGTPASSLVSKISAVQFPESADTTAKQSLG
SHRALTYTQSAPDLSPQILTPPVICSSCGRPYSQGNPADEPLERSGVATR
TPSRTDDTAQVTSDYETNNNSDSSDIVQNEDETECLREPLRKASACSTYA
PETMMFLDKPILAPEPLVMDNLDSIMEQLNTWNFPIFDLVENIGRKCGRI
LSQVSYRLFEDMGLFEAFKIPIREFMNYFHALEIGYRDIPYHNRIHATDV
LHAVWYLTTQPIPGLSTVINDHGSTSDSDSDSGFTHGHMGYVFSKTYNVT
DDKYGCLSGNIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYN
DRSVLENHHAAAAWNLFMSRPEYNFLINLDHVEFKHFRFLVIEAILATDL
KKHFDFVAKFNGKVNDDVGIDWTNENDRLLVCQMCIKLADINGPAKCKEL
HLQWTDGIVNEFYEQGDEEASLGLPISPFMDRSAPQLANLQESFISHIVG
PLCNSYDSAGLMPGKWVEDSDESGDTDDPEEEEEEAPAPNEEETCENNES
PKKKTFKRRKIYCQITQHLLQNHKMWKKVIEEEQRLAGIENQSLDQTPQS
HSSEQIQAIKEEEEEKGKPRGEEIPTQKPDQ*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU3602
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP for mammalian expression.