cDNA Clone CAPKK

• Plasmid: pEBG6P-CAPKK
• Parent Plasmid: pEBG6P
• Expressed: GST-CAPKK
• Genbank: NP_200863
• Tag: GST
• Species: Arabidopsis thaliana

Sequence of Insert

GCGGCCGCCAGTGTGATGGATATCTGCAGAATTCGCCCTTGCGAATTCTT
TCGTGATAGTTTTTTGTTTGCCCGTACCATCGGTTGCTTTGGATGCTTTG
GCAGTTCTGGTAGTAGAAACCAACAATCGCCTAAGCCCTACGATGATGAT
ACACATTCCTGTGATAGTGATGTAACTAGCACTGCAAGAGGAGAAGAAGA
AGAAGATGAAGAAGAAGTAGAGCAGAAGAGCCGCTCTAAACGATCTGAGG
AAATTTTGAAATATAGATTAGATAATGGATTAATCTGCAGACACATTCCA
GTGAGAGAAACTAATGAACTTATACGTGGAGAGGATGAAAATGGTGACAA
GACAATCAATGAGTATGTTCGCGTGTGTAAAATTGGATCCGGTAGCTATG
GTAAAGTGGTTTTATATCGAAGCACTTTAGACGGCCAGTATTATGCTATC
AAGGCATTTCACAAGTCGCATTTATTAAGGCTAAGGGTGGCACCGTCAGA
GACTGCTATGAGTGATGTTCTTCGTGAGGTTATGATTATGAAAATTTTGG
AGCATCCTAACATCGTTAACCTCATTGAGGTGATAGATGACCCTGAAACT
GATCATTTTTACATGGTTCTTGAATACGTTGATGGAAAATGGGTGTATGA
CGGTTCTGGACCGCCGGGTGCTCTCGGAGAGAAAACTGCTAGAAAGTATT
TGCGGGATATTGTTACTGGACTGATGTATCTTCATGCTCATGATGTTATT
CACGGGGACATTAAACCCGACATTTTGCTGGTTACTAGTAGCGGTACAGT
GAAGATAGGAGATTTCAGTGTCAGCCAAGTTTTCAAGGATGATGACGATC
AGCTGCGCCGATCTCCCGGGACTCCTGTCTTCACTGCTCCAGAATGTTGT
TTAGTATCAGGTATAACGTATAGTGGCAGAGCTGCGGATACATGGGCAGT
GGGAGTTACATTGTATTGTATGATATTGGGACAATACCCGTTTCTTGCGG
ATACTCTTCAGGACACCTACGACAAGATTGTTAACAACCCTCTGATAATC
CCGGATGGATTGAATCCTCTTCTCAGAGATTTGATCGAAGGTCTCCTTTG
CAAAGATCCGAGCCAGAGAATGACACTGAAGAATGTGTCGGAGCATCCAT
GGGTCATTGGAGAAGACGGGCATGTCCCGGAGTATTTTTGTTGGTGCAAA
CGCAATGCTGCCTCAAAGATTGAAGAAGGAGAAGCTAATGGGATCTCTGA
AACCTCAGATCCTAACTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAASVMD
ICRIRPCEFFRDSFLFARTIGCFGCFGSSGSRNQQSPKPYDDDTHSCDSD
VTSTARGEEEEDEEEVEQKSRSKRSEEILKYRLDNGLICRHIPVRETNEL
IRGEDENGDKTINEYVRVCKIGSGSYGKVVLYRSTLDGQYYAIKAFHKSH
LLRLRVAPSETAMSDVLREVMIMKILEHPNIVNLIEVIDDPETDHFYMVL
EYVDGKWVYDGSGPPGALGEKTARKYLRDIVTGLMYLHAHDVIHGDIKPD
NLLVTSSGTVKIGDFSVSQVFKDDDDQLRRSPGTPVFTAPECCLVSGITY
SGRAADTWAVGVTLYCMILGQYPFLADTLQDTYDKIVNNPLIIPDGLNPL
LRDLIEGLLCKDPSQRMTLKNVSEHPWVIGEDGHVPEYFCWCKRNAASKI
EEGEANGISETSDPN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3596
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression.