cDNA Clone p38 beta MAPK

• Synonyms: MAPK11
• Plasmid: pEGFP-C2-p38 beta MAPK D168A
• Parent Plasmid: pEGFPC2
• Expressed: GFP-p38 beta MAPK D168A
• Genbank: Y14440
• Tag: GFP
• Species: Human

Sequence of Insert

GAATTCATGTCGGGCCCTCGCGCCGGCTTCTACCGGCAGGAGCTGAACAA
GACCGTGTGGGAGGTGCCGCAGCGGCTGCAGGGGCTGCGCCCGGTGGGCT
CCGGCGCCTACGGCTCCGTTTGTTCGGCCTACGACGCCCGGCTGCGCCAG
AAGGTGGCGGTGAAGAAGCTGTCGCGCCCCTTCCAGTCGCTGATCCACGC
GCGCAGAACGTACCGGGAGCTGCGGCTGCTCAAGCACCTGAAGCACGAGA
ACGTCATCGGGCTTCTGGACGTCTTCACGCCGGCCACGTCCATCGAGGAC
TTCAGCGAAGTGTACTTGGTGACCACCCTGATGGGCGCCGACCTGAACAA
CATCGTCAAGTGCCAGGCGCTGAGCGACGAGCACGTTCAATTCCTGGTTT
ACCAGCTGCTGCGCGGGCTGAAGTACATCCACTCGGCCGGGATCATCCAC
CGGGACCTGAAGCCCAGCAACGTGGCTGTGAACGAGGACTGTGAGCTCAG
GATCCTGGCTTTCGGGCTGGCGCGCCAGGCGGACGAGGAGATGACCGGCT
ATGTGGCCACGCGCTGGTACCGGGCACCTGAGATCATGCTCAACTGGATG
CATTACAACCAAACAGTGGATATCTGGTCCGTGGGCTGCATCATGGCTGA
GCTGCTCCAGGGCAAGGCCCTCTTCCCGGGAAGCGACTACATTGACCAGC
TGAAGCGCATCATGGAAGTGGTGGGCACACCCAGCCCTGAGGTTCTGGCA
AAAATCTCCTCGGAACACGCCCGGACATATATCCAGTCCCTGCCCCCCAT
GCCCCAGAAGGACCTGAGCAGCATCTTCCGTGGAGCCAACCCCCTGGCCA
TAGACCTCCTTGGAAGGATGCTGGTGCTGGACAGTGACCAGAGGGTCAGT
GCAGCTGAGGCACTGGCCCACGCCTACTTCAGCCAGTACCACGACCCCGA
GGATGAGCCAGAGGCCGAGCCATATGATGAGAGCGTTGAGGCCAAGGAGC
GCACGCTGGAGGAGTGGAAGGAGCTCACTTACCAGGAAGTCCTCAGCTTC
AAGCCCCCAGAGCCACCGAAGCCACCTGGCAGCCTGGAGATTGAGCAGTG
AGGTGCTGCCCAGCAGCCCCTGAGAGCCTGGGGACCGGAATGTCCCGGGT
CG

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
EFMSGPRAGFYRQELNKTVWEVPQRLQGLRPVGSGAYGSVCSAYDARLRQ
KVAVKKLSRPFQSLIHARRTYRELRLLKHLKHENVIGLLDVFTPATSIED
FSEVYLVTTLMGADLNNIVKCQALSDEHVQFLVYQLLRGLKYIHSAGIIH
RDLKPSNVAVNEDCELRILAFGLARQADEEMTGYVATRWYRAPEIMLNWM
HYNQTVDIWSVGCIMAELLQGKALFPGSDYIDQLKRIMEVVGTPSPEVLA
KISSEHARTYIQSLPPMPQKDLSSIFRGANPLAIDLLGRMLVLDSDQRVS
AAEALAHAYFSQYHDPEDEPEAEPYDESVEAKERTLEEWKELTYQEVLSF
KPPEPPKPPGSLEIEQ*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU3573
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP tag for mammalian expression. Kinase Dead mutation.