cDNA Clone PINK1

• Plasmid: pGFP TAP BN PINK1
• Parent Plasmid: pGFP TAP BN
• Expressed: GFP-TAP-BN PINK1
• Genbank: BC028215
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

GGATCCGCGGTGCGACAGGCGCTGGGCCGCGGCCTGCAGCTGGGTCGAGC
GCTGCTGCTGCGCTTCACGGGCAAGCCCGGCCGGGCCTACGGCTTGGGGC
GGCCGGGCCCGGCGGCGGGCTGTGTCCGCGGGGAGCGTCCAGGCTGGGCC
GCAGGACCGGGCGCGGAGCCTCGCAGGGTCGGGCTCGGGCTCCCTAACCG
TCTCCGCTTCTTCCGCCAGTCGGTGGCCGGGCTGGCGGCGCGGTTGCAGC
GGCAGTTCGTGGTGCGGGCCTGGGGCTGCGCGGGCCCTTGCGGCCGGGCA
GTCTTTCTGGCCTTCGGGCTAGGGCTGGGCCTCATCGAGGAAAAACAGGC
GGAGAGCCGGCGGGCGGTCTCGGCCTGTCAGGAGATCCAGGCAATTTTTA
CCCAGAAAAGCAAGCCGGGGCCTGACCCGTTGGACACGAGACGCTTGCAG
GGCTTTCGGCTGGAGGAGTATCTGATAGGGCAGTCCATTGGTAAGGGCTG
CAGTGCTGCTGTGTATGAAGCCACCATGCCTACATTGCCCCAGAACCTGG
AGGTGACAAAGAGCACCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCAGT
GCACCAGGAGAAGGGCAGGAGCGAGCTGCGGGGGCCCCTGCCTTCCCCTT
GGCCATCAAGATGATGTGGAACATCTCGGCAGGTTCCTCCAGCGAAGCCA
TCTTGAACACAATGAGCCAGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTG
GCTGGGGAGTATGGAGCAGTCACTTACAGAAAATCCAAGAGAGGTCCCAA
GCAACTAGCCCCTCACCCCAACATCATCCGGGTTCTCCGCGCCTTCACCT
CTTCCGTGCCGCTGCTGCCAGGGGCCCTGGTCGACTACCCTGATGTGCTG
CCCTCACGCCTCCACCCTGAAGGCCTGGGCCATGGCCGGACGCTGTTCCT
CGTTATGAAGAACTATCCCTGTACCCTGCGCCAGTACCTTTGTGTGAACA
CACCCAGCCCCCGCCTCGCCGCCATGATGCTGCTGCAGCTGCTGGAAGGC
GTGGACCATCTGGTTCAACAGGGCATCGCGCACAGAGACCTGAAATCCGA
CAACATCCTTGTGGAGCTGGACCCAGACGGCTGCCCCTGGCTGGTGATCG
CAGATTTTGGCTGCTGCCTGGCTGATGAGAGCATCGGCCTGCAGTTGCCC
TTCAGCAGCTGGTACGTGGATCGGGGCGGAAACGGCTGTCTGATGGCCCC
AGAGGTGTCCACGGCCCGTCCTGGCCCCAGGGCAGTGATTGACTACAGCA
AGGCTGATGCCTGGGCAGTGGGAGCCATCGCCTATGAAATCTTCGGGCTT
GTCAATCCCTTCTACGGCCAGGGCAAGGCCCACCTTGAAAGCCGCAGCTA
CCAAGAGGCTCAGCTACCTGCACTGCCCGAGTCAGTGCCTCCAGACGTGA
GACAGTTGGTGAGGGCACTGCTCCAGCGAGAGGCCAGCAAGAGACCATCT
GCCCGAGTAGCCGCAAATGTGCTTCATCTAAGCCTCTGGGGTGAACATAT
TCTAGCCCTGAAGAATCTGAAGTTAGACAAGATGGTTGGCTGGCTCCTCC
AACAATCGGCCGCCACTTTGTTGGCCAACAGGCTCACAGAGAAGTGTTGT
GTGGAAACAAAAATGAAGATGCTCTTTCTGGCTAACCTGGAGTGTGAAAC
GCTCTGCCAGGCAGCCCTCCTCCTCTGCTCATGGAGGGCAGCCCTGTGAG
CGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSAVR
QALGRGLQLGRALLLRFTGKPGRAYGLGRPGPAAGCVRGERPGWAAGPGA
EPRRVGLGLPNRLRFFRQSVAGLAARLQRQFVVRAWGCAGPCGRAVFLAF
GLGLGLIEEKQAESRRAVSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLE
EYLIGQSIGKGCSAAVYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEG
QERAAGAPAFPLAIKMMWNISAGSSSEAILNTMSQELVPASRVALAGEYG
AVTYRKSKRGPKQLAPHPNIIRVLRAFTSSVPLLPGALVDYPDVLPSRLH
PEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLV
QQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWY
VDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFY
GQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAA
NVLHLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKM
KMLFLANLECETLCQAALLLCSWRAAL*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU3406
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP TAP for mammalian expression.