cDNA Clone MKK5

• Plasmid: pCR2.1 DN11 MKK5 S137A new
• Parent Plasmid: pGEX6P1
• Genbank: NM_003188 (TAK1) MAP3K7IP1 (TAB1)
• Species: Human

Sequence of Insert

GGATCCATGTCTACAGCCTCTGCCGCCTCCTCCTCCTCCTCGTCTTCGGC
CGGTGAGATGATCGAAGCCCCTTCCCAGGTCCTCAACTTTGAAGAGATCG
ACTACAAGGAGATCGAGGTGGAAGAGGTTGTTGGAAGAGGAGCCTTTGGA
GTTGTTTGCAAAGCTAAGTGGAGAGCAAAAGATGTTGCTATTAAACAAAT
AGAAAGTGAATCTGAGAGGAAAGCGTTTATTGTAGAGCTTCGGCAGTTAT
CCCGTGTGAACCATCCTAATATTGTAAAGCTTTATGGAGCCTGCTTGAAT
CCAGTGTGTCTTGTGATGGAATATGCTGAAGGGGGCTCTTTATATAATGT
GCTGCATGGTGCTGAACCATTGCCATATTATACTGCTGCCCACGCAATGA
GTTGGTGTTTACAGTGTTCCCAAGGAGTGGCTTATCTTCACAGCATGCAA
CCCAAAGCGCTAATTCACAGGGACCTGAAACCACCAAACTTACTGCTGGT
TGCAGGGGGGACAGTTCTAAAAATTTGTGATTTTGGTACAGCCTGTGACA
TTCAGACACACATGACCAATAACAAGGGGAGTGCTGCTTGGATGGCACCT
GAAGTTTTTGAAGGTAGTAATTACAGTGAAAAATGTGACGTCTTCAGCTG
GGGTATTATTCTTTGGGAAGTGATAACGCGTCGGAAACCCTTTGATGAGA
TTGGTGGCCCAGCTTTCCGAATCATGTGGGCTGTTCATAATGGTACTCGA
CCACCACTGATAAAAAATTTACCTAAGCCCATTGAGAGCCTGATGACTCG
TTGTTGGTCTAAAGATCCTTCCCAGCGCCCTTCAATGGAGGAAATTGTGA
AAATAATGACTCACTTGATGCGGTACTTTCCAGGAGCAGATGAGCCATTA
CAGTATCCTTGTCAGGAATTCGGAGGAGGAGGAGGAGAAATGAGCCAGCC
CACACCGAGCCCAGCCCCAGCTGCAGGAGGACGAGTGTACCCTGTGTCTG
TGCCATACTCCAGCGCCCAGAGCACCAGCAAGACCAGCGTGACCCTCTCC
CTTGTCATGCCCTCCCAGGGCCAGATGGTCAACGGGGCTCACAGTGCTTC
CACCCTGGACGAAGCCACCCCCACCCTCACCAACCAAAGCCCGACCTTAA
CCCTGCAGTCCACCAACACGCACACGCAGAGCAGCAGCTCCAGCTCTGAC
GGAGGCCTCTTCCGCTCCCGGCCCGCCCACTCGCTCCCGCCTGGCGAGGA
CGGTCGTGTTGAGCCCTATGTGGACTTTGCTGAGTTTTACCGCCTCTGGA
GCGTGGACCATGGCGAGCAGAGCGTGGTGACAGCACCGTAGGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSTASAASSSSSSSAGEMI
EAPSQVLNFEEIDYKEIEVEEVVGRGAFGVVCKAKWRAKDVAIKQIESES
ERKAFIVELRQLSRVNHPNIVKLYGACLNPVCLVMEYAEGGSLYNVLHGA
EPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKPPNLLLVAGGT
VLKICDFGTACDIQTHMTNNKGSAAWMAPEVFEGSNYSEKCDVFSWGIIL
WEVITRRKPFDEIGGPAFRIMWAVHNGTRPPLIKNLPKPIESLMTRCWSK
DPSQRPSMEEIVKIMTHLMRYFPGADEPLQYPCQEFGGGGGEMSQPTPSP
APAAGGRVYPVSVPYSSAQSTSKTSVTLSLVMPSQGQMVNGAHSASTLDE
ATPTLTNQSPTLTLQSTNTHTQSSSSSSDGGLFRSRPAHSLPPGEDGRVE
PYVDFAEFYRLWSVDHGEQSVVTAP*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU3343
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.