cDNA Clone p38 alpha MAPK

• Plasmid: pGEX-4T-SAPK2a T106M
• Parent Plasmid: pGEX4T1
• Expressed: GST-SAPK2a T106M
• Genbank: AAA57456
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTCTCAGGAGAGGCCCACGTTCTACCGGCAGGAGCTGAACAA
GACAATCTGGGAGGTGCCCGAGCGTTACCAGAACCTGTCTCCAGTGGGCT
CTGGCGCCTATGGCTCTGTGTGTGCTGCTTTTGACACAAAAACGGGGTTA
CGTGTGGCAGTGAAGAAGCTCTCCAGACCATTTCAGTCCATCATTCATGC
GAAAAGAACCTACAGAGAACTGCGGTTACTTAAACATATGAAACATGAAA
ATGTGATTGGTCTGTTGGACGTTTTTACACCTGCAAGGTCTCTGGAGGAA
TTCAATGATGTGTATCTGGTGATGCATCTCATGGGGGCAGATCTGAACAA
CATTGTGAAATGTCAGAAGCTTACAGATGACCATGTTCAGTTCCTTATCT
ACCAAATTCTCCGAGGTCTAAAGTATATACATTCAGCTGACATAATTCAC
AGGGACCTAAAACCTAGTAATCTAGCTGTGAATGAAGACTGTGAGCTGAA
GATTCTGGATTTTGGACTGGCTCGGCACACAGATGATGAAATGACAGGCT
ACGTGGCCACTAGGTGGTACAGGGCTCCTGAGATCATGCTGAACTGGATG
CATTACAACCAGACAGTTGATATTTGGTCAGTGGGATGCATAATGGCCGA
GCTGTTGACTGGAAGAACATTGTTTCCTGGTACAGACCATATTGATCAGT
TGAAGCTCATTTTAAGACTCGTTGGAACCCCAGGGGCTGAGCTTTTGAAG
AAAATCTCCTCAGAGTCTGCAAGAAACTATATTCAGTCTTTGACTCAGAT
GCCGAAGATGAACTTTGCGAATGTATTTATTGGTGCCAATCCCCTGGCTG
TCGACTTGCTGGAGAAGATGCTTGTATTGGACTCAGATAAGAGAATTACA
GCGGCCCAAGCCCTTGCACATGCCTACTTTGCTCAGTACCACGATCCTGA
TGATGAACCAGTGGCCGATCCTTATGATCAGTCCTTTGAAAGCAGGGACC
TCCTTATAGATGAGTGGAAAAGCCTGACCTATGATGAAGTCATCAGCTTT
GTGCCACCACCCCTTGACCAAGAAGAGATGGAGTCCTGAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSMSQERPTFYRQELNKTIWEVPERY
QNLSPVGSGAYGSVCAAFDTKTGLRVAVKKLSRPFQSIIHAKRTYRELRL
LKHMKHENVIGLLDVFTPARSLEEFNDVYLVMHLMGADLNNIVKCQKLTD
DHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNEDCELKILDFGLARH
TDDEMTGYVATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLTGRTLFP
GTDHIDQLKLILRLVGTPGAELLKKISSESARNYIQSLTQMPKMNFANVF
IGANPLAVDLLEKMLVLDSDKRITAAQALAHAYFAQYHDPDDEPVADPYD
QSFESRDLLIDEWKSLTYDEVISFVPPPLDQEEMES*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3315
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N Terminal GST fusion for bacterial expression.