cDNA Clone MKK4 (mouse)

• Plasmid: pGEX-4T-MKK4 D1-39 D245A
• Parent Plasmid: pGEX4T1
• Expressed: GST-MKK4 D1-39 D245A
• Genbank: U18310
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCATGCAGGGTAAGCGCAAAGCACTGAAGTTGAATTTTGCAAATCC
ACCTGTCAAATCGACAGCACGGTTTACCCTGAATCCTAATACTACAGGAG
TCCAGAACCCACACATAGAGAGACTGAGAACACACAGCATTGAGTCATCA
GGAAAACTGAAGATCTCCCCTGAACAACACTGGGATTTCACTGCAGAGGA
CTTGAAAGACCTTGGAGAAATTGGACGAGGAGCTTATGGTTCTGTCAACA
AAATGGTCCACAAACCAAGTGGGCAGATAATGGCAGTTAAAAGAATTCGG
TCAACTGTGGATGAAAAAGAACAAAAACAACTTCTCATGGATTTGGATGT
AGTAATGCGGAGTAGTGATTGCCCATACATTGTTCAGTTCTATGGTGCAC
TCTTCAGAGAGGGCGACTGTTGGATCTGTATGGAGCTCATGTCTACCTCG
TTCGATAAGTTTTACAAATATGTATATAGTGTGTTAGATGACGTTATTCC
GGAAGAGATCTTAGGCAAAATCACTTTAGCAACTGTGAAAGCACTAAACC
ACTTAAAAGAAAACTTGAAAATTATTCACAGAGACATCAAACCTTCCAAT
ATTCTTCTGGACAGAAGTGGAAATATAAAGCTCTGTGCTTTCGGCATCAG
TGGACAGCTTGTGGACTCTATTGCCAAGACAAGAGATGCTGGGTGTAGGC
CGTATATGGCACCTGAAAGAATAGACCCAAGTGCATCAAGACAAGGGTAT
GATGTCCGCTCTGATGTCTGGAGTTTGGGGATCACATTGTACGAGTTGGC
CACAGGCCGATTTCCTTATCCAAAGTGGAATAGTGTATTTGATCAGCTAA
CACAAGTGGTGAAAGGAGACCCTCCGCAGCTGAGTAATTCTGAAGAAAGG
GAGTTCTCCCCCAGTTTCATCAACTTTGTCAACTTGTGCCTTACGAAGGA
TGAATCCAAAAGGCCAAAGTATAAAGAGCTTCTGAAACATCCCTTTATTT
TGATGTATGAAGAACGTACTGTAGAGGTCGCATGCTATGTTTGTAAAATC
CTGGATCAGATGCCAGCCACTCCCAGCTCGCCCATGTATGTCGACTGAGG
ATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSMQGKRKALKLNFANPPVKSTARFT
LNPNTTGVQNPHIERLRTHSIESSGKLKISPEQHWDFTAEDLKDLGEIGR
GAYGSVNKMVHKPSGQIMAVKRIRSTVDEKEQKQLLMDLDVVMRSSDCPY
IVQFYGALFREGDCWICMELMSTSFDKFYKYVYSVLDDVIPEEILGKITL
ATVKALNHLKENLKIIHRDIKPSNILLDRSGNIKLCAFGISGQLVDSIAK
TRDAGCRPYMAPERIDPSASRQGYDVRSDVWSLGITLYELATGRFPYPKW
NSVFDQLTQVVKGDPPQLSNSEEREFSPSFINFVNLCLTKDESKRPKYKE
LLKHPFILMYEERTVEVACYVCKILDQMPATPSSPMYVD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3269
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.; Calc Mol Weight : 67565 Daltons