cDNA Clone MKK4 (mouse)

• Plasmid: pGEX-MKK4 KD1
• Parent Plasmid: pGEX4T1
• Expressed: GST-MKK4 KD1
• Genbank: U18310
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCATGCAGGGTAAGCGCAAAGCACTGAAGTTGAATTTTGCAAATCC
ACCTGTCAAATCGACAGCACGGTTTACCCTGAATCCTAATACTACAGGAG
TCCAGAACCCACACATAGAGAGACTGAGAACACACAGCATTGAGTCATCA
GGAAAACTGAAGATCTCCCCTGAACAACACTGGGATTTCACTGCAGAGGA
CTTGAAAGACCTTGGAGAAATTGGACGAGGAGCTTATGGTTCTGTCAACA
AAATGGTCCACAAACCAAGTGGGCAGATAATGGCAGTTAGAAGAATTCGG
TCAACTGTGGATGAAAAAGAACAAAAACAACTTCTTATGGATTTGGATGT
AGTAATGCGGAGTAGTGATTGCCCATACATTGTTCAGTTCTATGGTGCAC
TCTTCAGAGAGGGCGACTGTTGGATCTGTATGGAGCTCATGTCTACCTCG
TTCGATAAGTTTTACAAATATGTATATAGTGTGTTAGATGACGTTATTCC
GGAAGAGATCTTAGGCAAAATCACTTTAGCAACTGTGAAAGCACTAAACC
ACTTAAAAGAAAACTTGAAAATTATTCACAGAGACATCAAACCTTCCAAT
ATTCTTCTGGACAGAAGTGGAAATATAAAGCTCTGTGATTTCGGCATCAG
TGGACAGCTTGTGGACTCTATTGCCAAGACAAGAGATGCTGGGTGTAGGC
CGTATATGGCACCTGAAAGAATAGACCCAAGTGCATCAAGACAAGGGTAT
GATGTCCGCTCTGATGTCTGGAGTTTGGGGATCACATTGTACGAGTTGGC
CACAGGCCGATTTCCTTATCCAAAGTGGAATAGTGTATTTGATCAGCTAA
CACAAGTGGTGAAAGGAGACCCTCCGCAGCTGAGTAATTCTGAAGAAAGG
GAGTTCTCCCCCAGTTTCATCAACTTTGTCAACTTGTGCCTTACGAAGGA
TGAATCCAAAAGGCCAAAGTATAAAGAGCTTCTGAAACATCCCTTTATTT
TGATGTATGAAGAACGTACTGTAGAGGTCGCATGCTATGTTTGTAAAATC
CTGGATCAGATGCCAGCCACTCCCAGCTCGCCCATGTATGCCGACTGAGG
ATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSMQGKRKALKLNFANPPVKSTARFT
LNPNTTGVQNPHIERLRTHSIESSGKLKISPEQHWDFTAEDLKDLGEIGR
GAYGSVNKMVHKPSGQIMAVRRIRSTVDEKEQKQLLMDLDVVMRSSDCPY
IVQFYGALFREGDCWICMELMSTSFDKFYKYVYSVLDDVIPEEILGKITL
ATVKALNHLKENLKIIHRDIKPSNILLDRSGNIKLCDFGISGQLVDSIAK
TRDAGCRPYMAPERIDPSASRQGYDVRSDVWSLGITLYELATGRFPYPKW
NSVFDQLTQVVKGDPPQLSNSEEREFSPSFINFVNLCLTKDESKRPKYKE
LLKHPFILMYEERTVEVACYVCKILDQMPATPSSPMYAD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3199
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GSt for bacterial expression. Contains K129R and V396A mutations.