cDNA Clone FOXO1A

• Plasmid: pCMV5 FKHR T24A S319A
• Parent Plasmid: pEBG2T
• Expressed: HA-F FKHR T24A S319A
• Genbank: NM_002015
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGACGTCCCAGACTACGCTGCCGAGGC
GCCTCAGGTGGTGGAGATCGACCCGGACTTCGAGCCGCTGCCCCGGCCGC
GCTCGTGCGCCTGGCCGCTGCCCAGGCCGGAGTTTAGCCAGTCCAACTCG
GCCACCTCCAGCCCGGCGCCGTCGGGCAGCGCGGCTGCCAACCCCGACGC
CGCGGCGGGCCTGCCCTCGGCCTCGGCTGCCGCTGTCAGCGCCGACTTCA
TGAGCAACCTGAGCTTGCTGGAGGAGAGCGAGGACTTCCCGCAGGCGCCC
GGCTCCGTGGCGGCGGCGGTGGCGGCGGCGGCCGCCGCGGCCGCCACCGG
GGGGCTGTGCGGGGACTTCCAGGGCCCGGAGGCGGGCTGCCTGCACCCAG
CGCCACCGCAGCCCCCGCCGCCCGGGCCGCTGTCGCAGCACCCGCCGGTG
CCCCCCGCCGCCGCTGGGCCGCTCGCGGGGCAGCCGCGCAAGAGCAGCTC
GTCCCGCCGCAACGCGTGGGGCAACCTGTCCTACGCCGACCTCATCACCA
AGGCCATCGAGAGCTCGGCGGAGAAGCGGCTCACGCTGTCGCAGATCTAC
GAGTGGATGGTCAAGAGCGTGCCCTACTTCAAGGATAAGGGTGACAGCAA
CAGCTCGGCGGGCTGGAAGAATTCAATTCGTCATAATCTGTCCCTACACA
GCAAGTTCATTCGTGTGCAGAATGAAGGAACTGGAAAAAGTTCTTGGTGG
ATGCTCAATCCAGAGGGTGGCAAGAGCGGGAAATCTCCTAGGAGAAGAGC
TGCATCCATGGACAACAACAGTAAATTTGCTAAGAGCCGAAGCCGAGCTG
CCAAGAAGAAAGCATCTCTCCAGTCTGGCCAGGAGGGTGCTGGGGACAGC
CCTGGATCACAGTTTTCCAAATGGCCTGCAAGCCCTGGCTCTCACAGCAA
TGATGACTTTGATAACTGGAGTACATTTCGCCCTCGAACTAGCGCAAATG
CTAGTACTATTAGTGGGAGACTCTCACCCATTATGACCGAACAGGATGAT
CTTGGAGAAGGGGATGTGCATTCTATGGTGTACCCGCCATCTGCCGCAAA
GATGGCCTCTACTTTACCCAGTCTGTCTGAGATAAGCAATCCCGAAAACA
TGGAAAATCTTTTGGATAATCTCAACCTTCTCTCATCACCAACATCATTA
ACTGTTTCGACCCAGTCCTCACCTGGCACCATGATGCAGCAGACGCCGTG
CTACTCGTTTGCGCCACCAAACACCAGTTTGAATTCACCCAGCCCAAACT
ACCAAAAATATACATATGGCCAATCCAGCATGAGCCCTTTGCCCCAGATG
CCTATACAAACACTTCAGGACAATAAGTCGAGTTATGGAGGTATGAGTCA
GTATAACTGTGCGCCTGGACTCTTGAAGGAGTTGCTGACTTCTGACTCTC
CTCCCCATAATGACATTATGACACCAGTTGATCCTGGGGTAGCCCAGCCC
AACAGCCGGGTTCTGGGCCAGAACGTCATGATGGGCCCTAATTCGGTCAT
GTCAACCTATGGCAGCCAGGCATCTCATAACAAAATGATGAATCCCAGCT
CCCATACCCACCCTGGACATGCTCAGCAGACATCTGCAGTTAACGGGCGT
CCCCTGCCCCACACGGTAAGCACCATGCCCCACACCTCGGGTATGAACCG
CCTGACCCAAGTGAAGACACCTGTACAAGTGCCTCTGCCCCACCCCATGC
AGATGAGTGCCCTGGGGGGCTACTCCTCCGTGAGCAGCTGCAATGGCTAT
GGCAGAATGGGCCTTCTCCACCAGGAGAAGCTCCCAAGTGACTTGGATGG
CATGTTCATTGAGCGCTTAGACTGTGACATGGAATCCATCATTCGGAATG
ACCTCATGGATGGAGATACATTGGATTTTAACTTTGACAATGTGTTGCCC
AACCAAAGCTTCCCACACAGTGTCAAGACAACGACACATAGCTGGGTGTC
AGGCTGAGGGTTAGTGAGCAGGTTACACTTAAAAGTACTTCAGATTGTCT
GACAGCAGGAACTGAGAGAAGCAGTCCAAAGATGTCTTTCACCAACTCCC
TTTTAGTTTTCTCGGTTAAAAAAAAAAAAAAAAAAAAGGAATTCGATATC
AAGCTTATCGATACCGTCGACCCGGGCGGCCGCAAAGTTCCCTTTAGTGA
GGGTTAATGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACA
AACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTG
ATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTAAGCCGAATTCTGCAGATATCCATCACACTGGCGGCCGCT
CGAGCATGCATCTAGAGGATCC

Amino Acid Sequence

MYPYDVPDYAAEAPQVVEIDPDFEPLPRPRSCAWPLPRPEFSQSNSATSS
PAPSGSAAANPDAAAGLPSASAAAVSADFMSNLSLLEESEDFPQAPGSVA
AAVAAAAAAAATGGLCGDFQGPEAGCLHPAPPQPPPPGPLSQHPPVPPAA
AGPLAGQPRKSSSSRRNAWGNLSYADLITKAIESSAEKRLTLSQIYEWMV
KSVPYFKDKGDSNSSAGWKNSIRHNLSLHSKFIRVQNEGTGKSSWWMLNP
EGGKSGKSPRRRAASMDNNSKFAKSRSRAAKKKASLQSGQEGAGDSPGSQ
FSKWPASPGSHSNDDFDNWSTFRPRTSANASTISGRLSPIMTEQDDLGEG
DVHSMVYPPSAAKMASTLPSLSEISNPENMENLLDNLNLLSSPTSLTVST
QSSPGTMMQQTPCYSFAPPNTSLNSPSPNYQKYTYGQSSMSPLPQMPIQT
LQDNKSSYGGMSQYNCAPGLLKELLTSDSPPHNDIMTPVDPGVAQPNSRV
LGQNVMMGPNSVMSTYGSQASHNKMMNPSSHTHPGHAQQTSAVNGRPLPH
TVSTMPHTSGMNRLTQVKTPVQVPLPHPMQMSALGGYSSVSSCNGYGRMG
LLHQEKLPSDLDGMFIERLDCDMESIIRNDLMDGDTLDFNFDNVLPNQSF
PHSVKTTTHSWVSG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3108
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA tag for mammalian expression. Insert contains 3’ UTR.