cDNA Clone Ubiquitin

• Plasmid: pGEX6P-1-UBiquitin K63wt
• Parent Plasmid: pGEX6P1
• Expressed: GST-Ubiquitin K63wt
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCAGATCTTCGTGAGGACCCTGACTGGTAGGACCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAGGGCAAGGATCCAAGACA
GGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSQIFVRTLTGRTITLEVEPS
DTIENVRARIQDREGIPPDQQRLIFAGRQLEDGRTLSDYNIQKESTLHLV
LRLRGG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1989
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.