cDNA Clone MKK1 (rabbit)

• Synonyms: MAP2K1
• Plasmid: pGEX-MKK1 6His
• Parent Plasmid: pGEX3X
• Expressed: GST-MKK1 6His
• Genbank: CAA82912
• Tag: GST
• Species: Rabbit

Sequence of Insert

GGATCCCCCGATCCGCCATGCCAAAGAAGAAGCCCACCCCCATCCAGCTG
AATCCTGCCCCTGACGGCTCGGCGGTGAACGGCACCAGCTCGGCGGAGAC
CAACCTGGAGGCCTTGCAGAAGAAGCTGGAGGAGCTGGAGCTTGACGAGC
AGCAGCGGAAGCGCCTGGAGGCCTTCCTCACCCAGAAGCAGAAAGTGGGA
GAGCTGAAGGACGATGACTTCGAGAAGATCAGTGAGCTGGGAGCCGGCAA
CGGCGGCGTGGTGTTCAAGGTCTCCCACAAGCCGTCCGGCCTGGTCATGG
CCAGAAAGCTTATTCACCTGGAGATCAAACCTGCTATCCGGAACCAGATC
ATAAGGGAGCTGCAGGTTCTGCACGAGTGCAACTCCCCGTACATCGTGGG
CTTCTACGGGGCATTCTACAGCGATGGCGAGATCAGCATCTGCATGGAGC
ACATGGACGGGGGTTCCTTGGATCAAGTCCTGAAGAAAGCTGGACGGATT
CCCGAGCAAATTTTGGGGAAAGTTAGCATTGCTGTGATCAAGGGCCTGAC
GTATCTGAGGGAGAAGCACAAGATCATGCACAGAGACGTCAAGCCCTCCA
ACATCCTGGTCAACTCCCGCGGGGAGATCAAGCTCTGTGACTTCGGGGTC
AGTGGGCAGCTCATCGACTCCATGGCCAACTCCTTCGTGGGCACCAGGTC
TTATATGTCGCCCGAGAGACTCCAGGGGACACACTACTCTGTGCAGTCGG
ACATCTGGAGCATGGGGCTGTCCCTGGTGGAGATGGCGGTGGGGCGGTAC
CCCATCCCGCCCCCCGACGCCAAGGAGCTGGAGCTGATGTTTGGGTGCCA
GGTGGAGGGCGATGCGGCCGAGACTCCGCCCAGGCCCAGGACCCCTGGGC
GGCCCCTCAGCTCGTATGGAATGGATAGCCGGCCTCCCATGGCGATTTTT
GAGCTGCTGGATTACATCGTCAATGAGCCTCCTCCGAAACTCCCCAGCGC
AGTCTTCAGCCTGGAGTTTCAAGATTTTGTGAATAAATGCTTAATAAAAA
ACCCCGCCGAGAGAGCAGACTTGAAGCAGCTCATGGTTCATGCTTTTATC
AAGAGGTCTGATGCCGAGGAGGTGGATTTTGCTGGTTGGCTGTGCTCCAC
CATCGGCCTTAACCAGCCCAGCACGCCGACGCACGCGGCCGGTGTGCATC
ATCACCACCATCACTGA

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLIEGRGIPRSAMPKKKPTPIQLNPAPDGSA
VNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFE
KISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLH
ECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKV
SIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSM
ANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAK
ELELMFGCQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVN
EPPPKLPSAVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEV
DFAGWLCSTIGLNQPSTPTHAAGVHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1843
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST tag/ C terminal 6His tag.