cDNA Clone PI3K gamma

• Plasmid: pFBHb PI3K gamma new
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-Hb PI3Kgamma new
• Genbank: NM_002649
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGAGCTGGAGAACTATAAACAGCCCGTGGTGCTGAGAGAGGACAA
CTGCCGAAGGCGCCGGAGGATGAAGCCGCGCAGTGCTGCGGCCAGCCTGT
CCTCCATGGAGCTCATCCCCATCGAGTTCGTGCTGCCCACCAGCCAGCGC
AAATGCAAGAGCCCCGAAACGGCGCTGCTGCACGTGGCCGGCCACGGCAA
CGTGGAGCAGATGAAGGCCCAGGTGTGGCTGCGAGCGCTGGAGACCAGCG
TGGCGGCGGACTTCTACCACCGGCTGGGACCGCATCACTTCCTCCTGCTC
TATCAGAAGAAGGGGCAGTGGTACGAGATCTACGACAAGTACCAGGTGGT
GCAGACTCTGGACTGCCTGCGCTACTGGAAGGCCACGCACCGGAGCCCGG
GCCAGATCCACCTGGTGCAGCGGCACCCGCCCTCCGAGGAGTCCCAAGCC
TTCCAGCGGCAGCTCACGGCGCTGATTGGCTATGACGTCACTGACGTCAG
CAACGTGCACGACGATGAGCTGGAGTTCACGCGCCGTGGCTTGGTGACCC
CGCGCATGGCGGAGGTGGCCAGCCGCGACCCCAAGCTCTACGCCATGCAC
CCGTGGGTGACGTCCAAGCCCCTCCCGGAGTACCTGTGGAAGAAGATTGC
CAACAACTGCATCTTCATCGTCATTCACCGCAGCACCACCAGCCAGACCA
TTAAGGTCTCACCCGACGACACCCCCGGCGCCATCCTGCAGAGCTTCTTC
ACCAAGATGGCCAAGAAGAAATCTCTGATGGATATTCCCGAAAGCCAAAG
CGAACAGGATTTTGTGCTGCGCGTCTGTGGCCGGGATGAGTACCTGGTGG
GCGAAACGCCCATCAAAAACTTCCAGTGGGTGAGGCACTGCCTCAAGAAC
GGAGAAGAGATTCACGTGGTACTGGACACGCCTCCAGACCCGGCCCTAGA
CGAGGTGAGGAAGGAAGAGTGGCCGCTGGTGGATGACTGCACGGGAGTCA
CCGGCTACCATGAGCAGCTTACCATCCACGGCAAGGACCACGAGAGTGTG
TTCACCGTGTCCCTGTGGGACTGCGACCGCAAGTTCAGGGTCAAGATCAG
AGGCATTGATATCCCCGTCCTGCCTCGGAACACCGACCTCACAGTTTTTG
TAGAGGCAAACATCCAGCATGGGCAACAAGTCCTTTGCCAAAGGAGAACC
AGCCCCAAACCCTTCACAGAGGAGGTGCTGTGGAATGTGTGGCTTGAGTT
CAGTATCAAAATCAAAGACTTGCCCAAAGGGGCTCTACTGAACCTCCAGA
TCTACTGCGGTAAAGCTCCAGCACTGTCCAGCAAGGCCTCTGCAGAGTCC
CCCAGTTCTGAGTCCAAGGGCAAAGTTCAGCTTCTCTATTATGTGAACCT
GCTGCTGATAGACCACCGTTTCCTCCTGCGCCGTGGAGAATACGTCCTCC
ACATGTGGCAGATATCTGGGAAGGGAGAAGACCAAGGAAGCTTCAATGCT
GACAAACTCACGTCTGCAACTAACCCAGACAAGGAGAACTCAATGTCCAT
CTCCATTCTTCTGGACAATTACTGCCACCCGATAGCCCTGCCTAAGCATC
AGCCCACCCCTGACCCGGAAGGGGACCGGGTTCGAGCAGAAATGCCCAAC
CAGCTTCGCAAGCAATTGGAGGCGATCATAGCCACTGATCCACTTAACCC
TCTCACAGCAGAGGACAAAGAATTGCTCTGGCATTTTAGATACGAAAGCC
TTAAGCACCCAAAAGCATATCCTAAGCTATTTAGTTCAGTGAAATGGGGA
CAGCAAGAAATTGTGGCCAAAACATACCAATTGTTGGCCAGAAGGGAAGT
CTGGGATCAAAGTGCTTTGGATGTTGGGTTAACAATGCAGCTCCTGGACT
GCAACTTCTCAGATGAAAATGTAAGAGCCATTGCAGTTCAGAAACTGGAG
AGCTTGGAGGACGATGATGTTCTGCATTACCTTCTACAATTGGTCCAGGC
TGTGAAATTTGAACCATACCATGATAGCGCCCTTGCCAGATTTCTGCTGA
AGCGTGGTTTAAGAAACAAAAGAATTGGTCACTTTTTGTTTTGGTTCTTG
AGAAGTGAGATAGCCCAGTCCAGACACTATCAGCAGAGGTTCGCTGTGAT
TCTGGAAGCCTATCTGAGGGGCTGTGGCACAGCCATGCTGCACGACTTTA
CCCAACAAGTCCAAGTAATCGAGATGTTACAAAAAGTCACCCTTGATATT
AAATCGCTCTCTGCTGAAAAGTATGACGTCAGTTCCCAAGTTATTTCACA
ACTTAAACAAAAGCTTGAAAACCTGCAGAATTCTCAACTCCCCGAAAGCT
TTAGAGTTCCATATGATCCTGGACTGAAAGCAGGAGCGCTGGCAATTGAA
AAATGTAAAGTAATGGCCTCCAAGAAAAAACCACTATGGCTTGAGTTTAA
ATGTGCCGATCCTACAGCCCTATCAAATGAAACAATTGGAATTATCTTTA
AACATGGTGATGATCTGCGCCAAGACATGCTTATTTTACAGATTCTACGA
ATCATGGAGTCTATTTGGGAGACTGAATCTTTGGATCTATGCCTCCTGCC
ATATGGTTGCATTTCAACTGGTGACAAAATAGGAATGATCGAGATTGTGA
AAGACGCCACGACAATTGCCAAAATTCAGCAAAGCACAGTGGGCAACACG
GGAGCATTTAAAGATGAAGTCCTGAATCACTGGCTCAAAGAAAAATCCCC
TACTGAAGAAAAGTTTCAGGCAGCAGTGGAGAGATTTGTTTATTCCTGTG
CAGGCTACTGTGTGGCAACCTTTGTTCTTGGAATAGGCGACAGACACAAT
GACAATATTATGATCACCGAGACAGGAAACCTATTTCATATTGACTTCGG
GCACATTCTTGGGAATTACAAAAGTTTCCTGGGCATTAATAAAGAGAGAG
TGCCATTTGTGCTAACCCCTGACTTCCTCTTTGTGATGGGAACTTCTGGA
AAGAAGACAAGCCCACACTTCCAGAAATTTCAGGACATCTGTGTTAAGGC
TTATCTAGCCCTTCGTCATCACACAAACCTACTGATCATCCTGTTCTCCA
TGATGCTGATGACAGGAATGCCCCAGTTAACAAGCAAAGAAGACATTGAA
TATATCCGGGATGCCCTCACAGTGGGGAAAAATGAGGAGGATGCTAAAAA
GTATTTTCTTGATCAGATCGAAGTTTGCAGAGACAAAGGATGGACTGTGC
AGTTTAATTGGTTTCTACATCTTGTTCTTGGCATCAAACAAGGAGAGAAA
CATTCAGCCTAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSELENYKQPVVLREDNCRRRRRM
KPRSAAASLSSMELIPIEFVLPTSQRKCKSPETALLHVAGHGNVEQMKAQ
VWLRALETSVAADFYHRLGPHHFLLLYQKKGQWYEIYDKYQVVQTLDCLR
YWKATHRSPGQIHLVQRHPPSEESQAFQRQLTALIGYDVTDVSNVHDDEL
EFTRRGLVTPRMAEVASRDPKLYAMHPWVTSKPLPEYLWKKIANNCIFIV
IHRSTTSQTIKVSPDDTPGAILQSFFTKMAKKKSLMDIPESQSEQDFVLR
VCGRDEYLVGETPIKNFQWVRHCLKNGEEIHVVLDTPPDPALDEVRKEEW
PLVDDCTGVTGYHEQLTIHGKDHESVFTVSLWDCDRKFRVKIRGIDIPVL
PRNTDLTVFVEANIQHGQQVLCQRRTSPKPFTEEVLWNVWLEFSIKIKDL
PKGALLNLQIYCGKAPALSSKASAESPSSESKGKVQLLYYVNLLLIDHRF
LLRRGEYVLHMWQISGKGEDQGSFNADKLTSATNPDKENSMSISILLDNY
CHPIALPKHQPTPDPEGDRVRAEMPNQLRKQLEAIIATDPLNPLTAEDKE
LLWHFRYESLKHPKAYPKLFSSVKWGQQEIVAKTYQLLARREVWDQSALD
VGLTMQLLDCNFSDENVRAIAVQKLESLEDDDVLHYLLQLVQAVKFEPYH
DSALARFLLKRGLRNKRIGHFLFWFLRSEIAQSRHYQQRFAVILEAYLRG
CGTAMLHDFTQQVQVIEMLQKVTLDIKSLSAEKYDVSSQVISQLKQKLEN
LQNSQLPESFRVPYDPGLKAGALAIEKCKVMASKKKPLWLEFKCADPTAL
SNETIGIIFKHGDDLRQDMLILQILRIMESIWETESLDLCLLPYGCISTG
DKIGMIEIVKDATTIAKIQQSTVGNTGAFKDEVLNHWLKEKSPTEEKFQA
AVERFVYSCAGYCVATFVLGIGDRHNDNIMITETGNLFHIDFGHILGNYK
SFLGINKERVPFVLTPDFLFVMGTSGKKTSPHFQKFQDICVKAYLALRHH
TNLLIILFSMMLMTGMPQLTSKEDIEYIRDALTVGKNEEDAKKYFLDQIE
VCRDKGWTVQFNWFLHLVLGIKQGEKHSA*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1747
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His for baculovirus expression