cDNA Clone MAPKAPK2

• Plasmid: pGEX-MAPKAPK2
• Parent Plasmid: pGEX4T1
• Expressed: GST-MAPKAPK2
• Genbank: NM_032960
• Tag: GST
• Species: Human

Sequence of Insert

TTCCACGTCAAGTCCGGCCTGCAGATCAAGAAGAACGCCATCATCGATGA
CTACAAGGTCACCAGCCAGGTCCTGGGGCTGGGCATCAACGGCAAAGTTT
TGCAGATCTTCAACAAGAGGACCCAGGAGAAATTCGCCCTCAAAATGCTT
CAGGACTGCCCCAAGGCCCGCAGGGAGGTGGAGCTGCACTGGCGGGCCTC
CCAGTGCCCCGACATCGTACGGATCGTGGATGTGTACGAGAATCTGTACG
CAGGGAGGAAGTGCCTGCTGATTGTCATGGAATGTTTGGACGGTGGAGAA
CTCTTTAGCCGAATCCAGGATCGAGGAGACCAGGCATTCACAGAAAGAGA
AGCATCCGAAATCATGAAGAGCATCGGTGAGGCCATCCAGTATCTGCATT
CAATCAACATTGCCCATCGGGATGTCAAGCCTGAGAATCTCTTATACACC
TCCAAAAGGCCCAACGCCATCCTGAAACTCACTGACTTTGGCTTTGCCAA
GGAAACCACCAGCCACAACTCTTTGACCACTCCTTGTTATACACCGTACT
ATGTGGCTCCAGAAGTGCTGGGTCCAGAGAAGTATGACAAGTCCTGTGAC
ATGTGGTCCCTGGGTGTCATCATGTACATTCTGCTGTGTGGGTATCCCCC
CTTCTACTCCAACCACGGCCTTGCCATCTCTCCGGGCATGAAGACTCGCA
TCCGAATGGGCCAGTATGAATTTCCCAACCCAGAATGGTCAGAAGTATCA
GAGGAAGTGAAGATGCTCATTCGGAATCTGCTGAAAACAGAGCCCACCCA
GAGAATGACCATCACCGAGTTTATGAACCACCCTTGGATCATGCAATCAA
CAAAGGTCCCTCAAACCCCACTGCACACCAGCCGGGTCCTGAAGGAGGAC
AAGGAGCGGTGGGAGGATGTCAAGGAGGAGATGACCAGTGCCTTGGCCAC
AATGCGCGTTGACTACGAGCAGATCAAGATAAAAAAGATTGAAGATGCAT
CCAACCCTCTGCTGCTGAAGAGGCGGAAGAAAGCTCGGGCCCTGGAGGCT
GCGGCTCTGGGCCACATGGAGCAGAAGCTGATCAGCGAGGAGGACCTGAA
G

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNPLYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLHMFEDRLCHKTYLNGDHVTH
PDFMLYDALAVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSPGISGGGGGILEATMEFHVKSGLQ
IKKNAIIDDYKVTSQVLGLGINGKVLQIFNKRTQEKFALKMLQDCPKARR
EVELHWRASQCPHIVRIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDR
GDQAFTEREASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAIL
KLTDFGFAKETTSHNSLTTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIM
YILLCGYPPFYSNHGLAISPGMKTRIRMGQYEFPNPEWSEVSEEVKMLIR
NLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKEDKERWEDVK
EEMTSALATMRVDYEQIKIKKIEDASNPLLLKRRKKARALRAAALGHMEQ
KLISEEDLK

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1714
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST and C terminal Myc tags