cDNA Clone MEKK1

• Synonyms: MAP3K1
• Plasmid: pFBGST MEKK1 D1-622 human
• Parent Plasmid: pFastbac GST
• Expressed: 6His-GST MEKK1 D1-622 human
• Genbank: AF042838
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGTTACTACAGTACCCCATGTGTTTTCAAAACTGTTAGAAAT
GCTGAGTGTTTCCAGTTCCACTCACTTCACCAGGATGCGTCGCCGTTTGA
TGGCTATTGCAGATGAGGTGGAAATTGCCGAAGCCATCCAGTTGGGCGTA
GAAGACACTTTGGATGGTCAACAGGACAGCTTCTTGCAGGCATCTGTTCC
CAACAACTATCTGGAAACCACAGAGAACAGTTCCCCTGAGTGCACAATCC
ATTTAGAGAAAACTGGAAAAGGATTATGTGCTACAAAATTGAGTGCCAGT
TCAGAGGACATTTCTGAGAGACTGGCCAGCATTTCAGTAGGACCTTCTAG
TTCAACAACAACAACAACAACAACAGAGCAACCAAAGCCAATGGTTCAAA
CAAAAGGCAGACCCCACAGTCAGTGTTTGAACTCCTCTCCTTTATCTCAT
CATTCCCAATTAATGTTTCCAGCCTTGTCAACCCCTTCTTCTTCTACCCC
ATCTGTACCAGCTGGCACTGCAACAGATGTCTCTAAGCATAGACTTCAGG
GATTCATTCCCTGCAGAATACCTTCTGCATCTCCTCAAACACAGCGCAAG
TTTTCTCTACAATTCCACAGAAACTGTCCTGAAAACAAAGACTCAGATAA
ACTTTCCCCAGTCTTTACTCAGTCAAGACCCTTGCCCTCCAGTAACATAC
ACAGGCCAAAGCCATCTCGACCTACCCCAGGTAATACAAGTAAACAGGGA
GATCCCTCAAAAAATAGCATGACACTTGATCTGAACAGTAGTTCCAAATG
TGATGACAGCTTTGGCTGTAGCAGCAATAGTAGTAATGCTGTTATACCCA
GTGACGAGACAGTGTTCACCCCAGTAGAGGAGAAATGCAGATTAGATGTC
AATACAGAGCTCAACTCCAGTATTGAGGACCTTCTTGAAGCATCTATGCC
TTCAAGTGATACAACAGTAACTTTTAAGTCAGAAGTTGCTGTCCTGTCTC
CTGAAAAGGCTGAAAATGATGATACCTACAAAGATGATGTGAATCATAAT
CAAAAGTGCAAAGAGAAGATGGAAGCTGAAGAAGAAGAAGCTTTAGCAAT
TGCCATGGCAATGTCAGCGTCTCAGGATGCCCTCCCCATAGTTCCTCAGC
TGCAGGTTGAAAATGGAGAAGATATCATCATTATTCAACAGGATACACCA
GAGACTCTACCAGGACATACCAAAGCAAAACAACCGTATAGAGAAGACAC
TGAATGGCTGAAAGGTCAACAGATAGGCCTTGGAGCATTTTCTTCTTGTT
ATCAGGCTCAAGATGTGGGAACTGGAACTTTAATGGCTGTTAAACAGGTG
ACTTATGTCAGAAACACATCTTCTGAGCAAGAAGAAGTAGTAGAAGCACT
AAGAGAAGAGATAAGAATGATGAGCCATCTGAATCATCCAAACATCATTA
GGATGTTGGGAGCCACGTGTGAGAAGAGCAATTACAATCTCTTCATTGAA
TGGATGGCAGGGGGATCGGTGGCTCATTTGCTGAGTAAATATGGAGCCTT
CAAAGAATCAGTAGTTATTAACTACACTGAACAGTTACTCCGTGGCCTTT
CGTATCTCCATGAAAACCAAATCATTCACAGAGATGTCAAAGGTGCCAAT
TTGCTAATTGACAGCACTGGTCAGAGACTAAGAATTGCAGATTTTGGAGC
TGCAGCCAGGTTGGCATCAAAAGGAACTGGTGCAGGAGAGTTTCAGGGAC
AATTACTGGGGACAATTGCATTTATGGCACCTGAGGTACTAAGAGGTCAA
CAGTATGGAAGGAGCTGTGATGTATGGAGTGTTGGCTGTGCTATTATAGA
AATGGCTTGTGCAAAACCACCATGGAATGCAGAAAAACACTCCAATCATC
TTGCTTTGATATTTAAGATTGCTAGTGCAACTACTGCTCCATCGATCCCT
TCACATTTGTCTCCTGGTTTACGAGATGTGGCTCTTCGTTGTTTAGAACT
TCAACCTCAGGACAGACCTCCATCAAGAGAGCTACTGAAGCATCCAGTCT
TTCGTACTACATGGCATCATCACCATCACCATTGAGAATTCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMVTTVPHVFSKLLEMLSVS
SSTHFTRMRRRLMAIADEVEIAEAIQLGVEDTLDGQQDSFLQASVPNNYL
ETTENSSPECTIHLEKTGKGLCATKLSASSEDISERLASISVGPSSSTTT
TTTTEQPKPMVQTKGRPHSQCLNSSPLSHHSQLMFPALSTPSSSTPSVPA
GTATDVSKHRLQGFIPCRIPSASPQTQRKFSLQFHRNCPENKDSDKLSPV
FTQSRPLPSSNIHRPKPSRPTPGNTSKQGDPSKNSMTLDLNSSSKCDDSF
GCSSNSSNAVIPSDETVFTPVEEKCRLDVNTELNSSIEDLLEASMPSSDT
TVTFKSEVAVLSPEKAENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAM
SASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDTEWLK
GQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEI
RMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESV
VINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARL
ASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACA
KPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQD
RPPSRELLKHPVFRTTWHHHHHH*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1684
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST fusion/ C terminal 6His for baculovirus expression n.b. numbering in name relates to Celera protein hCP1814173. M1 of hCP1814173 is M145 in AF042838