cDNA Clone FCP1a

• Plasmid: pGEX6P-1-FCP1a delta C
• Parent Plasmid: pGEX6P1
• Expressed: GST-FCP1a delta C
• Genbank: NM_004715
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCGAGGTGCCGGCCGCGGGTCGCGTTCCTGCCGAGGGCGCCCCGAC
GGCGGCTGTGGCCGAGGTGCGCTGCCCGGGGCCCGCGCCGCTGCGCCTGC
TGGAGTGGAGGGTGGCGGCGGGCGCGGCCGTGCGCATCGGCTCGGTGCTG
GCCGTGTTCGAGGCCGCCGCCTCCGCGCAGTCCGCCGGGGCCTCTCAGTC
CCGTGTAGCCTCCGGGGGCTGCGTGCGCCCCGCGCGGCCGGAACGCAGGC
TGAGGTCGGAGCGCGCGGGCGTGGTGCGGGAGCTGTGCGCGCAGCCGGGC
CAGGTGGTCGCCCCAGGAGCGGTTCTGGTGAGGTTGGAAGGATGCAGCCA
CCCGGTTGTCATGAAAGGCCTGTGTGCTGAATGTGGCCAAGACCTCACCC
AGTTGCAGAGTAAGAACGGGAAGCAGCAGGTGCCGCTGTCCACGGCGACC
GTGTCCATGGTGCACAGCGTGCCGGAGTTGATGGTGAGCTCCGAGCAAGC
TGAACAGCTGGGAAGAGAAGACCAGCAGCGACTGCACCGAAACCGGAAGC
TGGTGCTCATGGTGGACTTGGACCAGACGTTGATTCACACAACCGAGCAG
CACTGTCAGCAGATGTCGAATAAAGGCATCTTTCACTTCCAGCTGGGCCG
GGGTGAGCCCATGCTGCACACGCGCCTGCGTCCACACTGCAAGGACTTCC
TGGAGAAGATCGCCAAGCTGTACGAGCTGCACGTCTTCACCTTCGGCAGC
CGGCTGTACGCACACACCATCGCAGGCTTTTTAGACCCCGAGAAGAAGCT
TTTTTCTCACCGAATATTATCAAGGGATGAATGTATTGACCCATTTTCTA
AAACGGGAAACCTTAGAAATCTCTTTCCTTGTGGAGACTCCATGGTTTGC
ATTATTGATGATCGAAAAGATGTCTGGAAGTTTGCCCCCAATCTGATAAC
TGTGAAGAAATATGTATACTTCCAGGGCACGGGTGATATGAATGCGCCCC
CTGGGTCCCGAGAATCTCAGACGAGAAAGAAAGTAAATCATTCTCGAGGC
ACTGAGGTCTCAGAGCCATCTCCGCCCGTGAGAGACCCTGAGGGGGTAAC
GCAGGCCCCTGGAGTGGAGCCCAGCAATGGCCTGGAGAAGCCTGCACGGG
AGCTGAACGGCAGCGAGGCCGCCACCCCGCGGGACTCACCCCGCCCCGGG
AAGCCAGACGAGAGGGACATCTGGCCCCCTGCCCAGGCCCCCACCAGCAG
CCAAGAGCTGGCAGGCGCTCCTGAGCCCCAGGGATCCTGTGCGCAGGGTG
GCCGGGTGGCACCGGGACAGCGGCCTGCCCAGGGTGCCACGGGCACTGAC
CTGGACTTTGACTTATCCAGCGACAGCGAGAGCAGCAGTGAGTCCGAGGG
CACGAAGTCCTCCTCCTCCGCCTCTGATGGCGAAAGCGAGGGGAAAAGAG
GCCGGCAGAAGCCGAAGGCTGCCCCAGAGGGAGCCGGGGCGCTGGCACAG
GGCAGTTCCCTGGAGCCGGGGCGGCCTGCAGCACCGAGTCTCCCCGGAGA
GGCCGAGCCTGGCGCGCATGCCCCGGACAAGGAGCCTGAGCTGGGTGGGC
AGGAGGAGGGCGAGCGGGATGGCCTCTGCGGCCTGGGCAACGGCTGTGCC
GACAGGAAGGAGGCGGAGACCGAGTCACAGAACAGCGAGCTGTCGGGGGT
CACTGCGGGTGAGTCCCTGGACCAGAGCATGGAGGAGGAGGAGGAGGAGG
ACACGGATGAGGATGACCACCTCATCTACCTGGAGGAGATCCTGGTCCGT
GTACACACTGACTACTATGCCAAGTATGACCGCTACCTCAACAAGGAGAT
CGAGGAGGCGCCGGACATCCGCAAGATCGTGCCGGAGCTCAAGAGCAAGG
TGCTGGCAGACGTGGCCATAATTTTCAGTGGGCTACACCCGACAAACTTC
CCGATAGAGAAGACGCGGGAGCATTACCACGCCACGGCGCTGGGAGCGAA
GATCCTCACTCGGCTGGTGCTGAGCCCCGACGCCCCTGACAGGGCCACGC
ACCTGATCGCCGCGCGAGCTGGCACAGAGAAGGTGCTGCAGGCACAGGAG
TGCGGACACCTGCACGTGGTCAACCCTGACTGGCTGTGGAGCTGCCTGGA
GCGCTGGGACAAGGTGGAGGAGCAGCTCTTCCCGCTCAGGGACGATCACA
CCAAGGCACAGAGGGAGAACAGCCCTGCGGCCTTTCCCGACCGGGAGGGT
GTGCCCCCCACCGCCTTGTTCCACCCGATGCCGGTTCTTCCCAAGGCCCA
GCCTGGCCCCGAGGTTCGGATCTACGACTCCAACACGGGGAAGCTCATCA
GGACGGGCGCCCGGGGGCCCCCAGCACCCTCCAGCTCCCTACCCATCCGC
CAGGAGCCCTCTTCCTTCAGAGCGGTTCCGCCACCCCAGCCGCAGATGTT
TGGTGAAGAGCTGCCTGACGCTCAGGACGGAGAGCAGCCTGGCCCTTCTA
GAAGAAAGCGACAGCCCAGTATGTCTGAGACAATGCCGCTGTACACTCTT
TGTAAGGAGGATTTAGAGAGTATGGACAAAGAGAGGCCACAAGAGGAAGC
TGAATGAAGAGGACGCCGCCAGCGAGTCCAGCAGGGAGTCCAGCAACGAG
GACGAGGGCAGCAGCTCCGAGGCCGACGAGATGGCCAAGGCGCTGGAGGC
GGAGCTCAACGACCTCATGTGAGAATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFEVPAAGRVPAEGAPTA
AVAEVRCPGPAPLRLLEWRVAAGAAVRIGSVLAVFEAAASAQSAGASQSR
VASGGCVRPARPERRLRSERAGVVRELCAQPGQVVAPGAVLVRLEGCSHP
VVMKGLCAECGQDLTQLQSKNGKQQVPLSTATVSMVHSVPELMVSSEQAE
QLGREDQQRLHRNRKLVLMVDLDQTLIHTTEQHCQQMSNKGIFHFQLGRG
EPMLHTRLRPHCKDFLEKIAKLYELHVFTFGSRLYAHTIAGFLDPEKKLF
SHRILSRDECIDPFSKTGNLRNLFPCGDSMVCIIDDRKDVWKFAPNLITV
KKYVYFQGTGDMNAPPGSRESQTRKKVNHSRGTEVSEPSPPVRDPEGVTQ
APGVEPSNGLEKPARELNGSEAATPRDSPRPGKPDERDIWPPAQAPTSSQ
ELAGAPEPQGSCAQGGRVAPGQRPAQGATGTDLDFDLSSDSESSSESEGT
KSSSSASDGESEGKRGRQKPKAAPEGAGALAQGSSLEPGRPAAPSLPGEA
EPGAHAPDKEPELGGQEEGERDGLCGLGNGCADRKEAETESQNSELSGVT
AGESLDQSMEEEEEEDTDEDDHLIYLEEILVRVHTDYYAKYDRYLNKEIE
EAPDIRKIVPELKSKVLADVAIIFSGLHPTNFPIEKTREHYHATALGAKI
LTRLVLSPDAPDRATHLIAARAGTEKVLQAQECGHLHVVNPDWLWSCLER
WDKVEEQLFPLRDDHTKAQRENSPAAFPDREGVPPTALFHPMPVLPKAQP
GPEVRIYDSNTGKLIRTGARGPPAPSSSLPIRQEPSSFRAVPPPQPQMFG
EELPDAQDGEQPGPSRRKRQPSMSETMPLYTLCKEDLESMDKERPQEEAE
*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1680
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST tag for bacterial expression. Protein is truncated at 860E and has RPQEEAE added before stop codon.