cDNA Clone GMD

• Plasmid: pEGFP-C1-HA-GMD
• Parent Plasmid: pEGFPC1
• Expressed: GFP-HA GMD
• Genbank: AF040260
• Tag: GFP
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGATGGTTC
CTACCTGGCTGAGTTCCTGCTGGAGAAAGGCTATGAGGTCCATGGAATTG
TACGGCGGTCCAGTTCATTTAATACGGGTCGAATTGAGCATCTGTATAAG
AATCCCCAGGCTCACATTGAAGGAAACATGAAGTTGCACTATGGCGATCT
CACTGACAGTACCTGCCTTGTGAAGATCATTAATGAAGTAAAGCCCACAG
AGATCTACAACCTTGGAGCCCAGAGCCACGTCAAAATTTCCTTTGACCTC
GCTGAGTACACTGCGGACGTTGACGGAGTTGGCACTCTACGACTTCTAGA
TGCAGTTAAGACTTGTGGCCTTATCAACTCTGTGAAGTTCTACCAAGCCT
CAACAAGTGAACTTTATGGGAAAGTGCAGGAAATACCCCAGAAGGAGACC
ACCCCTTTCTATCCCCGGTCACCCTATGGGGCAGCAAAACTCTATGCCTA
TTGGATTGTGGTGAACTTCCGTGAGGCGTATAATCTCTTTGCAGTGAACG
GCATTCTCTTCAATCATGAGAGTCCCAGAAGAGGAGCTAATTTCGTTACT
CGAAAAATTAGCCGGTCAGTAGCTAAGATTTACCTTGGACAACTGGAATG
TTTCAGTTTGGGAAATCTGGATGCCAAACGAGATTGGGGCCATGCCAAGG
ACTATGTGGAGGCTATGTGGTTGATGTTGCAGAATGATGAGCCGGAGGAC
TTCGTTATAGCTACTGGGGAGGTCCATAGTGTCCGGGAATTTGTCGAGAA
ATCATTCTTGCACATTGGAAAAACCATTGTGTGGGAAGGAAAGAATGAAA
ATGAAGTGGGCAGATGTAAAGAGACCGGCAAAGTTCACGTGACTGTGGAT
CTCAAGTACTACCGGCCAACTGAAGTGGACTTTCTGCAGGGCGACTGCAC
CAAAGCGAAACAGAAGCTGAACTGGAAGCCCCGGGTCGCTTTCGATGAGC
TGGTGAGGGAGATGGTGCACGCCGACGTGGAGCTCATGAGGACAAACCCC
AATGCCTGAGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSATMYPYDVPDYADGSYLAEFLLEKGYEVHGIVRRSSSFN
TGRIEHLYKNPQAHIEGNMKLHYGDLTDSTCLVKIINEVKPTEIYNLGAQ
SHVKISFDLAEYTADVDGVGTLRLLDAVKTCGLINSVKFYQASTSELYGK
VQEIPQKETTPFYPRSPYGAAKLYAYWIVVNFREAYNLFAVNGILFNHES
PRRGANFVTRKISRSVAKIYLGQLECFSLGNLDAKRDWGHAKDYVEAMWL
MLQNDEPEDFVIATGEVHSVREFVEKSFLHIGKTIVWEGKNENEVGRCKE
TGKVHVTVDLKYYRPTEVDFLQGDCTKAKQKLNWKPRVAFDELVREMVHA
DVELMRTNPNA*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU1676
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP/HA tag for Mammalian expression