cDNA Clone PCBP1

• Plasmid: pEGFP-C1-HA-PCBP1
• Parent Plasmid: pEGFPC1
• Expressed: GFP-HA PCBP1
• Genbank: NM_006196
• Tag: GFP
• Tag: HA
• Species: Human

Sequence of Insert

GTCGACGCCACCATGTACCCATACGATGTGCCAGATTACGCCGATGCCGG
TGTGACTGAAAGTGGACTAAATGTGACTCTCACCATTCGGCTTCTTATGC
ACGGAAAGGAAGTAGGAAGCATCATTGGGAAGAAAGGGGAGTCGGTTAAG
AGGATCCGCGAGGAGAGTGGCGCGCGGATCAACATCTCGGAGGGGAATTG
TCCGGAGAGAATCATCACTCTGACCGGCCCCACCAATGCCATCTTTAAGG
CTTTCGCTATGATCATCGACAAGCTGGAGGAAGATATCAACAGCTCCATG
ACCAACAGTACCGCGGCCAGCAGGCCCCCGGTCACCCTGAGGCTGGTGGT
GCCGGCCACCCAGTGCGGCTCCCTGATTGGGAAAGGCGGGTGTAAGATCA
AAGAGATCCGCGAGAGTACGGGGGCGCAGGTCCAGGTGGCGGGGGATATG
CTGCCCAACTCCACCGAGCGGGCCATCACCATCGCTGGCGTGCCGCAGTC
TGTCACCGAGTGTGTCAAGCAGATTTGCCTGGTCATGCTGGAGACGCTCT
CCCAGTCTCCGCAAGGGAGAGTCATGACCATTCCGTACCAGCCCATGCCG
GCCAGCTCCCCAGTCATCTGCGCGGGCGGCCAAGATCGGTGCAGCGACGC
TGTGGGCTACCCCCATGCCACCCATGACCTGGAGGGACCACCTCTAGATG
CCTACTCGATTCAAGGACAACACACCATTTCTCCGCTCGATCTGGCCAAG
CTGAACCAGGTGGCAAGACAACAGTCTCACTTTGCCATGATGCACGGCGG
GACCGGATTCGCCGGAATTGACTCCAGCTCTCCAGAGGTGAAAGGCTATT
GGGCAAGTTTGGATGCATCTACTCAAACCACCCATGAACTCACCATTCCA
AATAACTTAATTGGCTGCATAATCGGGCGCCAAGGCGCCAACATTAATGA
GATCCGCCAGATGTCCGGGGCCCAGATCAAAATTGCCAACCCAGTGGAAG
GCTCCTCTGGTAGGCAGGTTACTATCACTGGCTCTGCTGCCAGTATTAGT
CTGGCCCAGTATCTAATCAATGCCAGGCTTTCCTCTGAGAAGGGCATGGG
GTGCAGCTAGGTCGAC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDATMYPYDVPDYADAGVTESGLNVTLTIRLLMHGKEVGSIIGKKGES
VKRIREESGARINISEGNCPERIITLTGPTNAIFKAFAMIIDKLEEDINS
SMTNSTAASRPPVTLRLVVPATQCGSLIGKGGCKIKEIRESTGAQVQVAG
DMLPNSTERAITIAGVPQSVTECVKQICLVMLETLSQSPQGRVMTIPYQP
MPASSPVICAGGQDRCSDAVGYPHATHDLEGPPLDAYSIQGQHTISPLDL
AKLNQVARQQSHFAMMHGGTGFAGIDSSSPEVKGYWASLDASTQTTHELT
IPNNLIGCIIGRQGANINEIRQMSGAQIKIANPVEGSSGRQVTITGSAAS
ISLAQYLINARLSSEKGMGCS*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU1603
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP/HA tag for mammalian expression