cDNA Clone PCBP1

• Plasmid: pGEX6P-1-HA PCBP1
• Parent Plasmid: pGEX6P3
• Expressed: GST-HA PCBP1
• Genbank: NM_006196
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

GTCGACGCCACCATGTACCCATACGATGTGCCAGATTACGCCGATGCCGG
TGTGACTGAAAGTGGACTAAATGTGACTCTCACCATTCGGCTTCTTATGC
ACGGAAAGGAAGTAGGAAGCATCATTGGGAAGAAAGGGGAGTCGGTTAAG
AGGATCCGCGAGGAGAGTGGCGCGCGGATCAACATCTCGGAGGGGAATTG
TCCGGAGAGAATCATCACTCTGACCGGCCCCACCAATGCCATCTTTAAGG
CTTTCGCTATGATCATCGACAAGCTGGAGGAAGATATCAACAGCTCCATG
ACCAACAGTACCGCGGCCAGCAGGCCCCCGGTCACCCTGAGGCTGGTGGT
GCCGGCCACCCAGTGCGGCTCCCTGATTGGGAAAGGCGGGTGTAAGATCA
AAGAGATCCGCGAGAGTACGGGGGCGCAGGTCCAGGTGGCGGGGGATATG
CTGCCCAACTCCACCGAGCGGGCCATCACCATCGCTGGCGTGCCGCAGTC
TGTCACCGAGTGTGTCAAGCAGATTTGCCTGGTCATGCTGGAGACGCTCT
CCCAGTCTCCGCAAGGGAGAGTCATGACCATTCCGTACCAGCCCATGCCG
GCCAGCTCCCCAGTCATCTGCGCGGGCGGCCAAGATCGGTGCAGCGACGC
TGTGGGCTACCCCCATGCCACCCATGACCTGGAGGGACCACCTCTAGATG
CCTACTCGATTCAAGGACAACACACCATTTCTCCGCTCGATCTGGCCAAG
CTGAACCAGGTGGCAAGACAACAGTCTCACTTTGCCATGATGCACGGCGG
GACCGGATTCGCCGGAATTGACTCCAGCTCTCCAGAGGTGAAAGGCTATT
GGGCAAGTTTGGATGCATCTACTCAAACCACCCATGAACTCACCATTCCA
AATAACTTAATTGGCTGCATAATCGGGCGCCAAGGCGCCAACATTAATGA
GATCCGCCAGATGTCCGGGGCCCAGATCAAAATTGCCAACCCAGTGGAAG
GCTCCTCTGGTAGGCAGGTTACTATCACTGGCTCTGCTGCCAGTATTAGT
CTGGCCCAGTATCTAATCAATGCCAGGCTTTCCTCTGAGAAGGGCATGGG
GTGCAGCTAGGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDATMYPYDVPDYAD
AGVTESGLNVTLTIRLLMHGKEVGSIIGKKGESVKRIREESGARINISEG
NCPERIITLTGPTNAIFKAFAMIIDKLEEDINSSMTNSTAASRPPVTLRL
VVPATQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGVP
QSVTECVKQICLVMLETLSQSPQGRVMTIPYQPMPASSPVICAGGQDRCS
DAVGYPHATHDLEGPPLDAYSIQGQHTISPLDLAKLNQVARQQSHFAMMH
GGTGFAGIDSSSPEVKGYWASLDASTQTTHELTIPNNLIGCIIGRQGANI
NEIRQMSGAQIKIANPVEGSSGRQVTITGSAASISLAQYLINARLSSEKG
MGCS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1596
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST/HA tag for bacterial expression