cDNA Clone ETV1

• Plasmid: pEGFP-C2-HA-ER81
• Parent Plasmid: pEGFPC2
• Expressed: GFP-HA ER81
• Genbank: I38893
• Tag: GFP
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGATGGATT
TTATGACCAGCAAGTGCCTTACATGGTCACCAATAGTCAGCGTGGGAGAA
ATTGTAACGAGAAACCAACAAATGTCAGGAAAAGAAAATTCATTAACAGA
GATCTGGCTCATGATTCAGAAGAACTCTTTCAAGATCTAAGTCAATTACA
GGAAACATGGCTTGCAGAAGCTCAGGTACCTGACAATGATGAGCAGTTTG
TACCAGACTATCAGGCTGAAAGTTTGGCTTTTCATGGCCTGCCACTGAAA
ATCAAGAAAGAACCCCACAGTCCATGTTCAGAAATCAGCTCTGCCTGCAG
TCAAGAACAGCCCTTTAAATTCAGCTATGGAGAAAAGTCCCTGTACAATG
TCAGTGCCTATGATCAGAACCCACAAGTGGGAATGAGGCCCTCCAACCCC
CCCACACCATCCAGCACGCCAGTGTCCCCACTGCATCATGCATCTCCAAA
CTCAACTCATACACCGAAACCTGACCGGGCCTTCCCAGCTCACCTCCCTC
CATCGCAGTCCATACCAGATAGCAGCTACCCCATGGACCACAGATTTCGC
CGCCAGCTTTCTGAACCCTGTAACTCCTTTCCTCCTTTGCCGACGATGCC
AAGGGAAGGACGTCCTATGTACCAACGCCAGATGTCTGAGCCAAACATCC
CCTTCCCACCACAAGGCTTTAAGCAGGAGTACCACGACCCAGTGTATGAA
CACAACACCATGGTTGGCAGTGCGGCCAGCCAAAGCTTTCCCCCTCCTCT
GATGATTAAACAGGAACCCAGAGATTTTGCATATGACTCAGAAGTGCCTA
GCTGCCACTCCATTTATATGAGGCAAGAAGGCTTCCTGGCTCATCCCAGC
AGAACAGAAGGCTGTATGTTTGAAAAGGGCCCCAGGCAGTTTTATGATGA
CACCTGTGTTGTCCCAGAAAAATTCGATGGAGACATCAAACAAGAGCCAG
GAATGTATCGGGAAGGACCCACATACCAACGGCGAGGATCACTTCAGCTC
TGGCAGTTTTTGGTAGCTCTTCTGGATGACCCTTCAAATTCTCATTTTAT
TGCCTGGACTGGTCGAGGCATGGAATTTAAACTGATTGAGCCTGAAGAGG
TGGCCCGACGTTGGGGCATTCAGAAAAACAGGCCAGCTATGAACTATGAT
AAACTTAGCCGTTCACTCCGCTATTACTATGAGAAAGGAATTATGCAAAA
GGTGGCTGGAGAGAGATATGTCTACAAGTTTGTGTGTGATCCAGAAGCCC
TTTTCTCCATGGCCTTTCCAGATAATCAGCGTCCACTGCTGAAGACAGAC
ATGGAACGTCACATCAACGAGGAGGACACAGTGCCTCTTTCTCACTTTGA
TGAGAGCATGGCCTACATGCCGGAAGGGGGCTGCTGCAACCCCCACCCCT
ACAACGAAGGCTACGTGTATTAAGTCGAC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
EFATMYPYDVPDYADGFYDQQVPYMVTNSQRGRNCNEKPTNVRKRKFINR
DLAHDSEELFQDLSQLQETWLAEAQVPDNDEQFVPDYQAESLAFHGLPLK
IKKEPHSPCSEISSACSQEQPFKFSYGEKSLYNVSAYDQNPQVGMRPSNP
PTPSSTPVSPLHHASPNSTHTPKPDRAFPAHLPPSQSIPDSSYPMDHRFR
RQLSEPCNSFPPLPTMPREGRPMYQRQMSEPNIPFPPQGFKQEYHDPVYE
HNTMVGSAASQSFPPPLMIKQEPRDFAYDSEVPSCHSIYMRQEGFLAHPS
RTEGCMFEKGPRQFYDDTCVVPEKFDGDIKQEPGMYREGPTYQRRGSLQL
WQFLVALLDDPSNSHFIAWTGRGMEFKLIEPEEVARRWGIQKNRPAMNYD
KLSRSLRYYYEKGIMQKVAGERYVYKFVCDPEALFSMAFPDNQRPLLKTD
MERHINEEDTVPLSHFDESMAYMPEGGCCNPHPYNEGYVY*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU1523
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP/HA tag for mammalian expression