cDNA Clone MKK5

• Plasmid: pEBG-2T-MKK5 FL
• Parent Plasmid: pEBG2T
• Expressed: GST-MKK5 FL
• Genbank: BC008838
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAGAACCAGGTGCTGGTAATTCGCATCAAGATCCCAAATAG
TGGCGCGGTGGACTGGACAGTGCACTCCGGGCCGCAGTTACTCTTCAGGG
ATGTGCTGGATGTGATAGGCCAGGTTCTGCCTGAAGCAACAACTACAGCA
TTTGAATATGAAGATGAAGATGGTGATCGAATTACAGTGAGAAGTGATGA
GGAAATGAAGGCAATGCTGTCATATTATTATTCCACAGTAATGGAACAGC
AAGTAAATGGACAGTTAATAGAGCCTCTGCAGATATTTCCAAGAGCCTGC
AAGCCTCCTGGGGAACGGAACATACATGGCCTGAAGGTGAATACTCGGGC
CGGACCCTCTCAACACAGCAGCCCAGCAGTCTCAGATTCACTTCCAAGCA
ATAGCTTAAAGAAGTCTTCTGCTGAACTGAAAAAAATACTAGCCAATGGC
CAGATGAATGAACAAGACATACGATATCGGGACACTCTTGGTCATGGCAA
CGGAGGCACAGTCTACAAAGCATATCATGTCCCGAGTGGGAAAATATTAG
CTGTAAAGGTCATACTACTAGATATTACACTGGAACTTCAGAAGCAAATT
ATGTCTGAATTGGAAATTCTTTATAAGTGCGATTCATCATATATCATTGG
ATTTTATGGAGCATTTTTTGTAGAAAACAGGATTTCAATATGTACAGAAT
TCATGGATGGGGGATCTTTGGATGTATATAGGAAAATGCCAGAACATGTC
CTTGGAAGAATTGCAGTAGCAGTTGTTAAAGGCCTTACTTATTTGTGGAG
TTTAAAGATTTTACATAGAGACGTGAAGCCCTCCAATATGCTAGTAAACA
CAAGAGGACAGGTTAAGCTGTGTGATTTTGGAGTTAGCACTCAGCTGGTG
AATTCTATAGCCAAGACGTATGTTGGAACAAATGCTTATATGGCGCCTGA
AAGGATTTCAGGGGAGCAGTATGGAATTCATTCTGATGTCTGGAGCTTAG
GAATCTCTTTTATGGAGCTTGCTCTTGGGAGGTTTCCATATCCTCAGATT
CAGAAAAACCAGGGATCTTTAATGCCTCTCCAGCTTCTGCAGTGCATTGT
TGATGAGGATTCGCCCGTCCTTCCAGTTGGAGAGTTCTCGGAGCCATTTG
TACATTTCATCACTCAGTGTATGCGAAAACAGCCAAAAGAAAGGCCAGCA
CCTGAAGAATTGATGGGCCACCCGTTCATCGTGCAGTTCAATGATGGAAA
TGCCGCCGTGGTGTCCATGTGGGTGTGCCGGGCGCTGGAGGAGAGGCGGA
GCCAGCAGGGGCCCCCGTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSMENQVLVIRIKIPNSGAVDWTVHSG
PQLLFRDVLDVIGQVLPEATTTAFEYEDEDGDRITVRSDEEMKAMLSYYY
STVMEQQVNGQLIEPLQIFPRACKPPGERNIHGLKVNTRAGPSQHSSPAV
SDSLPSNSLKKSSAELKKILANGQMNEQDIRYRDTLGHGNGGTVYKAYHV
PSGKILAVKVILLDITLELQKQIMSELEILYKCDSSYIIGFYGAFFVENR
ISICTEFMDGGSLDVYRKMPEHVLGRIAVAVVKGLTYLWSLKILHRDVKP
SNMLVNTRGQVKLCDFGVSTQLVNSIAKTYVGTNAYMAPERISGEQYGIH
SDVWSLGISFMELALGRFPYPQIQKNQGSLMPLQLLQCIVDEDSPVLPVG
EFSEPFVHFITQCMRKQPKERPAPEELMGHPFIVQFNDGNAAVVSMWVCR
ALEERRSQQGPP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1516
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST tag for mammalian expression