cDNA Clone MKK1

• Synonyms: MAP2K1
• Plasmid: pFBHTb MKK1 S217E S221E
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-MKK1 S217E S221E
• Genbank: L05624
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCCCCAAGAAGAAGCCGACGCCCATCCAGCTGAACCCGGCCCCCGA
CGGCTCTGCAGTTAACGGGACCAGCTCTGCGGAGACCAACTTGGAGGCCT
TGCAGAAGAAGCTGGAGGAGCTAGAGCTTGATGAGCAGCAGCGAAAGCGC
CTTGAGGCCTTTCTTACCCAGAAGCAGAAGGTGGGAGAACTGAAGGATGA
CGACTTTGAGAAGATCAGTGAGCTGGGGGCTGGCAATGGCGGTGTGGTGT
TCAAGGTCTCCCACAAGCCTTCTGGCCTGGTCATGGCCAGAAAGCTAATT
CATCTGGAGATCAAACCCGCAATCCGGAACCAGATCATAAGGGAGCTGCA
GGTTCTGCATGAGTGCAACTCTCCGTACATCGTGGGCTTCTATGGTGCGT
TCTACAGCGATGGCGAGATCAGTATCTGCATGGAGCACATGGATGGAGGT
TCTCTGGATCAAGTCCTGAAGAAAGCTGGAAGAATTCCTGAACAAATTTT
AGGAAAAGTTAGCATTGCTGTAATAAAAGGCCTGACATATCTGAGGGAGA
AGCACAAGATCATGCACAGAGATGTCAAGCCCTCCAACATCCTAGTCAAC
TCCCGTGGGGAGATCAAGCTCTGTGACTTTGGGGTCAGCGGGCAGCTCAT
CGACGAAATGGCCAACGAATTCGTGGGCACAAGGTCCTACATGTCGCCAG
AAAGACTCCAGGGGACTCATTACTCTGTGCAGTCAGACATCTGGAGCATG
GGACTGTCTCTGGTAGAGATGGCGGTTGGGAGGTATCCCATCCCTCCTCC
AGATGCCAAGGAGCTGGAGCTGATGTTTGGGTGCCAGGTGGAAGGAGATG
CGGCTGAGACCCCACCCAGGCCAAGGACCCCCGGGAGGCCCCTTAGCTCA
TACGGAATGGACAGCCGACCTCCCATGGCAATTTTTGAGTTGTTGGATTA
CATAGTCAACGAGCCTCCTCCAAAACTGCCCAGTGGAGTGTTCAGTCTGG
AATTTCAAGATTTTGTGAATAAATGCTTAATAAAAAACCCCGCAGAGAGA
GCAGATTTGAAGCAACTCATGGTTCATGCTTTTATCAAGAGATCTGATGC
TGAGGAAGTGGATTTTGCAGGTTGGCTCTGCTCCACCATCGGCCTTAACC
AGCCCAGCACACCAACCCATGCTGCTGGCGTCTAAGGATCC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSPKKKPTPIQLNPAPDGSAVNGT
SSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKISE
LGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNS
PYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAV
IKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDEMANEF
VGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELEL
MFGCQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPP
KLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAG
WLCSTIGLNQPSTPTHAAGV

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU934
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression with N terminal 6His tag