cDNA Clone MKK1

• Synonyms: MAP2K1
• Plasmid: pGEX6P-1-MKK1 6His S217E S221E
• Parent Plasmid: pGEX6P1
• Expressed: GST-MKK1 6His S217E S221E
• Genbank: NM_002755.3*
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCCCAAGAAGAAGCCGACGCCCATCCAGCTGAACCCGGCCCCCGA
CGGCTCTGCAGTTAACGGGACCAGCTCTGCGGAGACCAACTTGGAGGCCT
TGCAGAAGAAGCTGGAGGAGCTAGAGCTTGATGAGCAGCAGCGAAAGCGC
CTTGAGGCCTTTCTTACCCAGAAGCAGAAGGTGGGAGAACTGAAGGATGA
CGACTTTGAGAAGATCAGTGAGCTGGGGGCTGGCAATGGCGGTGTGGTGT
TCAAGGTCTCCCACAAGCCTTCTGGCCTGGTCATGGCCAGAAAGCTAATT
CATCTGGAGATCAAACCCGCAATCCGGAACCAGATCATAAGGGAGCTGCA
GGTTCTGCATGAGTGCAACTCTCCGTACATCGTGGGCTTCTATGGTGCGT
TCTACAGCGATGGCGAGATCAGTATCTGCATGGAGCACATGGATGGAGGT
TCTCTGGATCAAGTCCTGAAGAAAGCTGGAAGAATTCCTGAACAAATTTT
AGGAAAAGTTAGCATTGCTGTAATAAAAGGCCTGACATATCTGAGGGAGA
AGCACAAGATCATGCACAGAGATGTCAAGCCCTCCAACATCCTAGTCAAC
TCCCGTGGGGAGATCAAGCTCTGTGACTTTGGGGTCAGCGGGCAGCTCAT
CGACGAAATGGCCAACGAATTCGTGGGCACAAGGTCCTACATGTCGCCAG
AAAGACTCCAGGGGACTCATTACTCTGTGCAGTCAGACATCTGGAGCATG
GGACTGTCTCTGGTAGAGATGGCGGTTGGGAGGTATCCCATCCCTCCTCC
AGATGCCAAGGAGCTGGAGCTGATGTTTGGGTGCCAGGTGGAAGGAGATG
CGGCTGAGACCCCACCCAGGCCAAGGACCCCCGGGAGGCCCCTTAGCTCA
TACGGAATGGACAGCCGACCTCCCATGGCAATTTTTGAGTTGTTGGATTA
CATAGTCAACGAGCCTCCTCCAAAACTGCCCAGTGGAGTGTTCAGTCTGG
AATTTCAAGATTTTGTGAATAAATGCTTAATAAAAAACCCCGCAGAGAGA
GCAGATTTGAAGCAACTCATGGTTCATGCTTTTATCAAGAGATCTGATGC
TGAGGAAGTGGATTTTGCAGGTTGGCTCTGCTCCACCATCGGCCTTAACC
AGCCCAGCACACCAACCCATGCTGCTGGCGTCCATCATCACCATCACCAT
TAAGGATC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPKKKPTPIQLNPAPDGSAV
NGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEK
ISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHE
CNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVS
IAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDEMA
NEFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKE
LELMFGCQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNE
PPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVD
FAGWLCSTIGLNQPSTPTHAAGVHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU927
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: * Additional residue changes compared with reference: G328R and C376Y. Extra KGEFQHTGGRY residues after 6His tag. For clone with these residue changes corrected to wild-type and stop immediately after 6His tag see DU58892.
• Low Temp: No