cDNA Clone MKK1

• Synonyms: MAP2K1
• Plasmid: pFBHTb MKK1 S218D S222D
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-MKK1 S218D S222D
• Genbank: L05624
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCCCCAAGAAGAAGCCGACGCCCATCCAGCTGAACCCGGCCCCCGA
CGGCTCTGCAGTTAACGGGACCAGCTCTGCGGAGACCAACTTGGAGGCCT
TGCAGAAGAAGCTGGAGGAGCTAGAGCTTGATGAGCAGCAGCGAAAGCGC
CTTGAGGCCTTTCTTACCCAGAAGCAGAAGGTGGGAGAACTGAAGGATGA
CGACTTTGAGAAGATCAGTGAGCTGGGGGCTGGCAATGGCGGTGTGGTGT
TCAAGGTCTCCCACAAGCCTTCTGGCCTGGTCATGGCCAGAAAGCTAATT
CATCTGGAGATCAAACCCGCAATCCGGAACCAGATCATAAGGGAGCTGCA
GGTTCTGCATGAGTGCAACTCTCCGTACATCGTGGGCTTCTATGGTGCGT
TCTACAGCGATGGCGAGATCAGTATCTGCATGGAGCACATGGATGGAGGT
TCTCTGGATCAAGTCCTGAAGAAAGCTGGAAGAATTCCTGAACAAATTTT
AGGAAAAGTTAGCATTGCTGTAATAAAAGGCCTGACATATCTGAGGGAGA
AGCACAAGATCATGCACAGAGATGTCAAGCCCTCCAACATCCTAGTCAAC
TCCCGTGGGGAGATCAAGCTCTGTGACTTTGGGGTCAGCGGGCAGCTCAT
CGACGATATGGCCAACGATTTCGTGGGCACAAGGTCCTACATGTCGCCAG
AAAGACTCCAGGGGACTCATTACTCTGTGCAGTCAGACATCTGGAGCATG
GGACTGTCTCTGGTAGAGATGGCGGTTGGGAGGTATCCCATCCCTCCTCC
AGATGCCAAGGAGCTGGAGCTGATGTTTGGGTGCCAGGTGGAAGGAGATG
CGGCTGAGACCCCACCCAGGCCAAGGACCCCCGGGAGGCCCCTTAGCTCA
TACGGAATGGACAGCCGACCTCCCATGGCAATTTTTGAGTTGTTGGATTA
CATAGTCAACGAGCCTCCTCCAAAACTGCCCAGTGGAGTGTTCAGTCTGG
AATTTCAAGATTTTGTGAATAAATGCTTAATAAAAAACCCCGCAGAGAGA
GCAGATTTGAAGCAACTCATGGTTCATGCTTTTATCAAGAGATCTGATGC
TGAGGAAGTGGATTTTGCAGGTTGGCTCTGCTCCACCATCGGCCTTAACC
AGCCCAGCACACCAACCCATGCTGCTGGCGTCTAAGGATCC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSPKKKPTPIQLNPAPDGSAVNGT
SSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKISE
LGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNS
PYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAV
IKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDDMANDF
VGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELEL
MFGCQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPP
KLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAG
WLCSTIGLNQPSTPTHAAGV

• Vector Type: Baculovirus
• Antibiotic: Amp
• Full Snapgene Map: pFBHTb MKK1 S218D S222D45.98 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU910
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression with N terminal 6His tag
• Low Temp: No