cDNA Clone PDK1v

• Plasmid: pFBHTb d1-50 PDK1
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-d1-50 PDK1
• Genbank: O15530
• Tag: 6His
• Species: Human

Sequence of Insert

MSYYHHHHHHDYDIPTTENLYFQGAMGSATMEQKLISEEDLDGTAAEPRP
GAGSLQHAQPPPQPRKKRPEDFKFGKILGEGSFSTVVLARELATSREYAI
KILEKRHIIKENKVPYVTRERDVMSRLDHPFFVKLYFTFQDDEKLYFGLS
YAKNGELLKYIRKIGSFDETCTRFYTAEIVSALEYLHGKGIIHRDLKPEN
ILLNEDMHIQITDFGTAKVLSPESKQARANSFVGTAQYVSPELLTEKSAC
KSSDLWALGCIIYQLVAGLPPFRAGNEYLIFQKIIKLEYDFPEKFFPKAR
DLVEKLLVLDATKRLGCEEMEGYGPLKAHPFFESVTWENLHQQTPPKLTA
YLPAMSEDDEDCYGNYDNLLSQFGCMQVSSSSSSHSLSASDTGLPQRSGS
NIEQYIHDLDSNSFELDLQFSEDEKRLLLEKQAGGNPWHQFVENNLILKM
GPVDKRKGLFARRRQLLLTEGPHLYYVDPVNKVLKGEIPWSQELRPEAKN
FKTFFVHTPNRTYYLMDPSGNAHKWCRKIQEVWRQRYQSHPDAAVQ*

Amino Acid Sequence

GACGGCACTGCAGCCGAGCCTCGGCCCGGCGCCGGCTCCCTGCAGCATGC
CCAGCCTCCGCCGCAGCCTCGGAAGAAGCGGCCTGAGGACTTCAAGTTTG
GGAAAATCCTTGGGGAAGGCTCTTTTTCCACGGTTGTCCTGGCTCGAGAA
CTGGCAACCTCCAGAGAATATGCGATTAAAATTCTGGAGAAGCGACATAT
CATAAAAGAGAACAAGGTCCCCTATGTAACCAGAGAGCGGGATGTCATGT
CGCGCCTGGATCACCCCTTCTTTGTTAAGCTTTACTTCACATTTCAGGAC
GACGAGAAGCTGTATTTCGGCCTTAGTTATGCCAAAAATGGAGAACTACT
TAAATATATTCGCAAAATCGGTTCATTCGATGAGACCTGTACCCGATTTT
ACACGGCTGAGATCGTGTCTGCTTTAGAGTACTTGCACGGCAAGGGCATC
ATTCACAGGGACCTTAAACCGGAAAACATTTTGTTAAATGAAGATATGCA
CATCCAGATCACAGATTTTGGAACAGCAAAAGTCTTATCCCCAGAGAGCA
AACAAGCCAGGGCCAACTCATTCGTGGGAACAGCGCAGTACGTTTCTCCA
GAGCTGCTCACGGAGAAGTCCGCCTGTAAGAGTTCAGACCTTTGGGCTCT
TGGATGCATAATATACCAGCTTGTGGCAGGACTCCCACCATTCCGAGCTG
GAAACGAGTATCTTATATTTCAGAAGATCATTAAGTTGGAATATGACTTT
CCAGAAAAATTCTTCCCTAAGGCAAGAGACCTCGTGGAGAAACTTTTGGT
TTTAGATGCCACAAAGCGGTTAGGCTGTGAGGAAATGGAAGGATACGGAC
CTCTTAAAGCACACCCGTTCTTCGAGTCCGTCACGTGGGAGAACCTGCAC
CAGCAGACGCCTCCGAAGCTCACCGCTTACCTGCCGGCTATGTCGGAAGA
CGACGAGGACTGCTATGGCAATTATGACAATCTCCTGAGCCAGTTTGGCT
GCATGCAGGTGTCTTCGTCCTCCTCCTCACACTCCCTGTCAGCCTCCGAC
ACGGGCCTGCCCCAGAGGTCAGGCAGCAACATAGAGCAGTACATTCACGA
TCTGGACTCGAACTCCTTTGAACTGGACTTACAGTTTTCCGAAGATGAGA
AGAGGTTGTTGTTGGAGAAGCAGGCTGGCGGAAACCCTTGGCACCAGTTT
GTAGAAAATAATTTAATACTAAAGATGGGCCCAGTGGATAAGCGGAAGGG
TTTATTTGCAAGACGACGACAGCTGTTGCTCACAGAAGGACCACATTTAT
ATTATGTGGATCCTGTCAACAAAGTTCTGAAAGGTGAAATTCCTTGGTCA
CAAGAACTTCGACCAGAGGCCAAGAATTTTAAAACTTTCTTTGTCCACAC
GCCTAACAGGACGTATTATCTGATGGACCCCAGCGGGAACGCACACAAGT
GGTGCAGGAAGATCCAGGAGGTTTGGAGGCAGCGATACCAGAGCCACCCG
GACGCCGCTGTGCAGTGA

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU831
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression with N terminal 6His/myc tag