cDNA Clone PDE3A

• Plasmid: pCMV5-HA PDE3a frag
• Parent Plasmid: pCMV5
• Expressed: HA-PDE3a frag
• Genbank: M91667
• Tag: HA
• Species: Human

Sequence of Insert

MYPYDVPDYAGVLMIALTSAVRTVSLISLERFKVAWRPYLAYLAGVLGIL
LARYVEQILPQSAEAAPREHLGSQLIAGTKEDIPVFKRRRRSSSVVSAEM
SGCSSKSHRRTSLPCIPREQLMGHSEWDHKRGPRGSQSSGTSITVDIAVM
GEATASLPTSWQTLLFHQTCATSLRAVSNLLSTQLTFQAIHKPRVNPVTS
LSENYTCSDSEESSEKDKLAIPKRLRRSLPPGLLRRVSSTWTTTTSATGL
PTLEPAPVRRDRSTSIKLQEAPSSSPDSWNNPVMMTLT*

Amino Acid Sequence

GAATTCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGGCGTCCT
CATGATCGCCTTGACTAGCGCGGTCAGGACCGTGTCCCTCATTTCCTTAG
AGAGGTTCAAGGTCGCCTGGAGACCTTACCTGGCGTACCTGGCCGGCGTG
CTGGGGATCCTCTTGGCCAGGTACGTGGAACAAATCTTGCCGCAGTCCGC
GGAGGCGGCTCCAAGGGAGCATTTGGGGTCCCAGCTGATTGCTGGGACCA
AGGAAGATATCCCGGTGTTTAAGAGGAGGAGGCGGTCCAGCTCCGTCGTG
TCCGCCGAGATGTCCGGCTGCAGCAGCAAGTCCCATCGGAGGACCTCCCT
GCCCTGTATACCGAGGGAACAGCTCATGGGGCATTCAGAATGGGACCACA
AACGAGGGCCAAGAGGATCACAGTCTTCAGGAACCAGTATTACTGTGGAC
ATCGCCGTCATGGGCGAAGCCACGGCCTCATTACCGACCTCCTGGCAGAC
CCTTCTCTTCCACCAAACGTGTGCCACATCCTTGAGAGCCGTGAGCAACT
TGCTCAGCACACAGCTCACCTTCCAGGCCATTCACAAGCCCAGAGTGAAT
CCCGTTACTTCGCTCAGTGAAAACTATACCTGTTCTGACTCTGAAGAGAG
CTCTGAAAAAGACAAGCTTGCTATTCCAAAGCGCCTGAGAAGGAGTTTGC
CTCCTGGCTTGTTGAGACGAGTTTCTTCCACTTGGACCACCACCACCTCG
GCCACAGGTCTACCCACCTTGGAGCCTGCACCAGTACGGAGAGACCGCAG
CACCAGCATCAAACTGCAGGAAGCACCTTCATCCAGTCCTGATTCTTGGA
ATAATCCAGTGATGATGACCCTCACCA

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU828
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA tag for mammalian expression