cDNA Clone MEKK3

• Synonyms: MAP3K3
• Plasmid: pFastBac1 MEKK3D322
• Parent Plasmid: pFastbac1 mod
• Expressed: 6His MEKK3D322
• Genbank: U78876
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCTTTGGTCCCTCGTGGAAGCGGTAGCGATCGGGGGAAGCTCCTGGG
TCAAGGTGCCTTCCGCAGGGTCTACTTGTGCTATGATGTGGACACAGGAC
GTGAACTTGCTTCTAAGCAGGTCCAGTTTGACCCAGATAGTCCTGAGACA
AGCAAGGAGGTGAGTGCTCTGGAGTGTGAGATCCAGTTGCTGAAGAACCT
GCAGCATGAGCGCATTGTGCAGTACTACGGCTGCCTGCGGGACCGTGCTG
AGAAGATCCTCACCATCTTTATGGAGTATATGCCAGGGGGCTCTGTAAAA
GACCAGTTGAAGGCCTACGGAGCTCTGACAGAGAGTGTGACCCGCAAGTA
CACCCGGCAGATTCTGGAGGGCATGTCATACCTGCACAGCAACATGATTG
TGCATCGGGACATCAAGGGAGCCAATATCCTCCGAGACTCAGCTGGGAAT
GTGAAGCTTGGGGATTTTGGGGCCAGCAAACGCCTACAGACCATCTGCAT
GTCAGGGACAGGCATTCGCTCTGTCACTGGCACACCCTACTGGATGAGTC
CTGAAGTCATCAGTGGCGAGGGCTATGGAAGAAAGGCAGACGTGTGGAGC
CTGGGCTGTACTGTGGTGGAAATGCTGACAGAGAAACCACCTTGGGCAGA
GTATGAAGCTATGGCTGCCATTTTCAAGATTGCCACCCAGCCTACCAATC
CTCAGCTGCCCTCTCACATCTCAGAACACGGCAGGGACTTCCTGAGGCGC
ATATTTGTGGAAGCTCGTCAGAGACCCTCAGCTGAGGAGCTGCTCACACA
CCACTTTGCACAGCTAGTGTACTGA

Amino Acid Sequence

MAHHHHHHGSLVPRGSGSDRGKLLGQGAFRRVYLCYDVDTGRELASKQVQ
FDPDSPETSKEVSALECEIQLLKNLQHERIVQYYGCLRDRAEKILTIFME
YMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRDIKGAN
ILRDSAGNVKLGDFGASKRLQTICMSGTGIRSVTGTPYWMSPEVISGEGY
GRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSHISE
HGRDFLRRIFVEARQRPSAEELLTHHFAQLVY*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU789
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His construct for baculoviral expression