cDNA Clone MKK4

• Plasmid: pGEX6P1-MKK4 S257E T261E
• Parent Plasmid: pGEX6P1
• Expressed: GST-C3-MKK4 S257E T261E
• Genbank: NM_003010
• Tag: GST
• Species: Human

Sequence of Insert

agcatgcagggtaaacgcaaagcactgaagttgaattttgcaaatccacc
tttcaaatctacagcaaggtttactctgaatcccaatcctacaggagttc
aaaacccacacatagagagactgagaacacacagcattgagtcatcagga
aaactgaagatctcccctgaacaacactgggatttcactgcagaggactt
gaaagaccttggagaaattggacgaggagcttatggttctgtcaacaaaa
tggtccacaaaccaagtgggcaaataatggcagttaaaagaattcggtca
acagtggatgaaaaagaacaaaaacaacttcttatggatttggatgtagt
aatgcggagtagtgattgcccatacattgttcagttttatggtgcactct
tcagagagggtgactgttggatctgtatggaactcatgtctacctcgttt
gataagttttacaaatatgtatatagtgtattagatgatgttattccaga
agaaattttaggcaaaatcactttagcaactgtgaaagcactaaaccact
taaaagaaaacttgaaaattattcacagagatatcaaaccttccaatatt
cttctggacagaagtggaaatattaagctctgtgacttcggcatcagtgg
acagcttgtggacGAAattgccaagGAAagagatgctggctgtaggccat
acatggcacctgaaagaatagacccaagcgcatcacgacaaggatatgat
gtccgctctgatgtctggagtttggggatcacattgtatgagttggccac
aggccgatttccttatccaaagtggaatagtgtatttgatcaactaacac
aagtcgtgaaaggagatcctccgcagctgagtaattctgaggaaagggaa
ttctccccgagtttcatcaactttgtcaacttgtgccttacgaaggatga
atccaaaaggccaaagtataaagagcttctgaaacatccctttattttga
tgtatgaagaacgtgccgttgaggtcgcatgctatgtttgtaaaatcctg
gatcaaatgccagctactcccagctctcccatgtatgtcgattga

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSMQGKRKALKLNFANPPFK
STARFTLNPNPTGVQNPHIERLRTHSIESSGKLKISPEQHWDFTAEDLKD
LGEIGRGAYGSVNKMVHKPSGQIMAVKRIRSTVDEKEQKQLLMDLDVVMR
SSDCPYIVQFYGALFREGDCWICMELMSTSFDKFYKYVYSVLDDVIPEEI
LGKITLATVKALNHLKENLKIIHRDIKPSNILLDRSGNIKLCDFGISGQL
VDEIAKERDAGCRPYMAPERIDPSASRQGYDVRSDVWSLGITLYELATGR
FPYPKWNSVFDQLTQVVKGDPPQLSNSEEREFSPSFINFVNLCLTKDESK
RPKYKELLKHPFILMYEERAVEVACYVCKILDQMPATPSSPMYVD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU730
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST tag.