cDNA Clone MKK2

• Synonyms: MAP2K2
• Plasmid: pGEX6P1-MKK26His
• Parent Plasmid: pGEX6P1
• Expressed: GST-C3-MKK26His
• Genbank: P36507
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCTGGCCCGGAGGAAGCCGGTGCTGCCGGCGCTCACCATCAACCC
TACCATCGCCGAGGGCCCATCCCCTACCAGCGAGGGCGCCTCCGAGGCAA
ACCTGGTGGACCTGCAGAAGAAGCTGGAGGAGCTGGAACTTGACGAGCAG
CAGAAGAAGCGGCTGGAAGCCTTTCTCACCCAGAAAGCCAAGGTTGGCGA
ACTCAAAGACGATGACTTCGAAAGGATCTCAGAGCTGGGCGCGGGCAACG
GCGGGGTGGTCACCAAAGTCCAGCACAGACCCTCGGGCCTCATCATGGCC
AGGAAGCTGATCCACCTTGAGATCAAGCCGGCCATCCGGAACCAGATCAT
CCGCGAGCTGCAGGTCCTGCACGAATGCAACTCGCCGTACATCGTGGGCT
TCTACGGGGCCTTCTACAGTGACGGGGAGATCAGCATTTGCATGGAACAC
ATGGACGGCGGCTCCCTGGACCAGGTGCTGAAAGAGGCCAAGAGGATTCC
CGAGGAGATCCTGGGGAAAGTCAGCATCGCGGTTCTCCGGGGCTTGGCGT
ACCTCCGAGAGAAGCACCAGATCATGCACCGAGATGTGAAGCCCTCCAAC
ATCCTCGTGAACTCTAGAGGGGAGATCAAGCTGTGTGACTTCGGGGTGAG
CGGCCAGCTCATAGACTCCATGGCCAACTCCTTCGTGGGCACGCGCTCCT
ACATGGCTCCGGAGCGGTTGCAGGGCACACATTACTCGGTGCAGTCGGAC
ATCTGGAGCATGGGCCTGTCCCTGGTGGAGCTGGCCGTCGGAAGGTACCC
CATCCCCCCGCCCGACGCCAAAGAGCTGGAGGCCATCTTTGGCCGGCCCG
TGGTCGACGGGGAAGAAGGAGAGCCTCACAGCATCTCGCCTCGGCCGAGG
CCCCCCGGGCGCCCCGTCAGCGGTCACGGGATGGATAGCCGGCCTGCCAT
GGCCATCTTTGAACTCCTGGACTATATTGTGAACGAGCCACCTCCTAAGC
TGCCCAACGGTGTGTTCACCCCCGACTTCCAGGAGTTTGTCAATAAATGC
CTCATCAAGAACCCAGCGGAGCGGGCGGACCTGAAGATGCTCACAAACCA
CACCTTCATCAAGCGGTCCGAGGTGGAAGAAGTGGATTTTGCCGGCTGGT
TGTGTAAAACCCTGCGGCTGAACCAGCCCGGCACACCCACGCGCACCGCC
GTGCACCATCACCATCACCATTAAGAATTCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSLARRKPVLPALTINPTIAE
GPSPTSEGASEANLVDLQKKLEELELDEQQKKRLEAFLTQKAKVGELKDD
DFERISELGAGNGGVVTKVQHRPSGLIMARKLIHLEIKPAIRNQIIRELQ
VLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKEAKRIPEEIL
GKVSIAVLRGLAYLREKHQIMHRDVKPSNILVNSRGEIKLCDFGVSGQLI
DSMANSFVGTRSYMAPERLQGTHYSVQSDIWSMGLSLVELAVGRYPIPPP
DAKELEAIFGRPVVDGEEGEPHSISPRPRPPGRPVSGHGMDSRPAMAIFE
LLDYIVNEPPPKLPNGVFTPDFQEFVNKCLIKNPAERADLKMLTNHTFIK
RSEVEEVDFAGWLCKTLRLNQPGTPTRTAVHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU727
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST tag and C terminal 6His tag.