cDNA Clone MKK5

• Plasmid: pEBG-2T-delta N11 MKK5 D300A S310D T314D
• Parent Plasmid: pEBG2T
• Expressed: GST-delta N11 MKK5 D300A S310D T314D
• Genbank: BC008838
• Tag: GST
• Species: Human

Sequence of Insert

atggagaaccaggtgctggtaattcgcatcaagatcccaaatagtggcgc
ggtggactggacagtgcactccgggccgcagttactcttcagggatgtgc
tggatgtgataggccaggttctgcctgaagcaacaactacagcatttgaa
tatgaagatgaagatggtgatcgaattacagtgagaagtgatgaggaaat
gaaggcaatgctgtcatattattattccacagtaatggaacagcaagtaa
atggacagttaatagagcctctgcagatatttccaagagcctgcaagcct
cctggggaacggaacatacatggcctgaaggtgaatactcgggccggacc
ctctcaacacagcagcccagcagtctcagattcacttccaagcaatagct
taaagaagtcttctgctgaactgaaaaaaatactagccaatggccagatg
aatgaacaagacatacgatatcgggacactcttggtcatggcaacggagg
cacagtctacaaagcatatcatgtcccgagtgggaaaatattagctgtaa
aggtcatactactagatattacactggaacttcagaagcaaattatgtct
gaattggaaattctttataagtgcgattcatcatatatcattggatttta
tggagcattttttgtagaaaacaggatttcaatatgtacagaattcatgg
atgggggatctttggatgtatataggaaaatgccagaacatgtccttgga
agaattgcagtagcagttgttaaaggccttacttatttgtggagtttaaa
gattttacatagagacgtgaagccctccaatatgctagtaaacacaagag
gacaggttaagctgtgtgcttttggagttagcactcagctggtgaatgac
atagccaaggactatgttggaacaaatgcttatatggcgcctgaaaggat
ttcaggggagcagtatggaattcattctgatgtctggagcttaggaatct
cttttatggagcttgctcttgggaggtttccatatcctcagattcagaaa
aaccagggatctttaatgcctctccagcttctgcagtgcattgttgatga
ggattcgcccgtccttccagttggagagttctcggagccatttgtacatt
tcatcactcagtgtatgcgaaaacagccaaaagaaaggccagcacctgaa
gaattgatgggccacccgttcatcgtgcagttcaatgatggaaatgccgc
cgtggtgtccatgtgggtgtgccgggcgctggaggagaggcggagccagc
aggggcccccgtga

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSMENQVLVIRIKIPNSGAVDWTVHS
GPQLLFRDVLDVIGQVLPEATTTAFEYEDEDGDRITVRSDEEMKAMLSYY
YSTVMEQQVNGQLIEPLQIFPRACKPPGERNIHGLKVNTRAGPSQHSSPA
VSDSLPSNSLKKSSAELKKILANGQMNEQDIRYRDTLGHGNGGTVYKAYH
VPSGKILAVKVILLDITLELQKQIMSELEILYKCDSSYIIGFYGAFFVEN
RISICTEFMDGGSLDVYRKMPEHVLGRIAVAVVKGLTYLWSLKILHRDVK
PSNMLVNTRGQVKLCAFGVSTQLVNDIAKDYVGTNAYMAPERISGEQYGI
HSDVWSLGISFMELALGRFPYPQIQKNQGSLMPLQLLQCIVDEDSPVLPV
GEFSEPFVHFITQCMRKQPKERPAPEELMGHPFIVQFNDGNAAVVSMWVC
RALEERRSQQGPP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU646
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal GST tag