cDNA Clone MKK5

• Plasmid: pCMV5-FLAG MKK5 D300A
• Parent Plasmid: pCMV5
• Expressed: FLAG-MKK5 D300A
• Genbank: BC008838
• Tag: FLAG
• Species: Human

Sequence of Insert

ATGCTGTGGCTAGCCCTTGGCCCCTTTCCTGCCATGGAGAACCAGGTGCT
GGTAATTCGCATCAAGATCCCAAATAGTGGCGCGGTGGACTGGACAGTGC
ACTCCGGGCCGCAGTTACTCTTCAGGGATGTGCTGGATGTGATAGGCCAG
GTTCTGCCTGAAGCAACAACTACAGCATTTGAATATGAAGATGAAGATGG
TGATCGAATTACAGTGAGAAGTGATGAGGAAATGAAGGCAATGCTGTCAT
ATTATTATTCCACAGTAATGGAACAGCAAGTAAATGGACAGTTAATAGAG
CCTCTGCAGATATTTCCAAGAGCCTGCAAGCCTCCTGGGGAACGGAACAT
ACATGGCCTGAAGGTGAATACTCGGGCCGGACCCTCTCAACACAGCAGCC
CAGCAGTCTCAGATTCACTTCCAAGCAATAGCTTAAAGAAGTCTTCTGCT
GAACTGAAAAAAATACTAGCCAATGGCCAGATGAATGAACAAGACATACG
ATATCGGGACACTCTTGGTCATGGCAACGGAGGCACAGTCTACAAAGCAT
ATCATGTCCCGAGTGGGAAAATATTAGCTGTAAAGGTCATACTACTAGAT
ATTACACTGGAACTTCAGAAGCAAATTATGTCTGAATTGGAAATTCTTTA
TAAGTGCGATTCATCATATATCATTGGATTTTATGGAGCATTTTTTGTAG
AAAACAGGATTTCAATATGTACAGAATTCATGGATGGGGGATCTTTGGAT
GTATATAGGAAAATGCCAGAACATGTCCTTGGAAGAATTGCAGTAGCAGT
TGTTAAAGGCCTTACTTATTTGTGGAGTTTAAAGATTTTACATAGAGACG
TGAAGCCCTCCAATATGCTAGTAAACACAAGAGGACAGGTTAAGCTGTGT
GCTTTTGGAGTTAGCACTCAGCTGGTGAATTCTATAGCCAAGACGTATGT
TGGAACAAATGCTTATATGGCGCCTGAAAGGATTTCAGGGGAGCAGTATG
GAATTCATTCTGATGTCTGGAGCTTAGGAATCTCTTTTATGGAGCTTGCT
CTTGGGAGGTTTCCATATCCTCAGATTCAGAAAAACCAGGGATCTTTAAT
GCCTCTCCAGCTTCTGCAGTGCATTGTTGATGAGGATTCGCCCGTCCTTC
CAGTTGGAGAGTTCTCGGAGCCATTTGTACATTTCATCACTCAGTGTATG
CGAAAACAGCCAAAAGAAAGGCCAGCACCTGAAGAATTGATGGGCCACCC
GTTCATCGTGCAGTTCAATGATGGAAATGCCGCCGTGGTGTCCATGTGGG
TGTGCCGGGCGCTGGAGGAGAGGCGGAGCCAGCAGGGGCCCCCGTGA

Amino Acid Sequence

MDYKDDDDKLWLALGPFPAMENQVLVIRIKIPNSGAVDWTVHSGPQLLFR
DVLDVIGQVLPEATTTAFEYEDEDGDRITVRSDEEMKAMLSYYYSTVMEQ
QVNGQLIEPLQIFPRACKPPGERNIHGLKVNTRAGPSQHSSPAVSDSLPS
NSLKKSSAELKKILANGQMNEQDIRYRDTLGHGNGGTVYKAYHVPSGKIL
AVKVILLDITLELQKQIMSELEILYKCDSSYIIGFYGAFFVENRISICTE
FMDGGSLDVYRKMPEHVLGRIAVAVVKGLTYLWSLKILHRDVKPSNMLVN
TRGQVKLCAFGVSTQLVNSIAKTYVGTNAYMAPERISGEQYGIHSDVWSL
GISFMELALGRFPYPQIQKNQGSLMPLQLLQCIVDEDSPVLPVGEFSEPF
VHFITQCMRKQPKERPAPEELMGHPFIVQFNDGNAAVVSMWVCRALEERR
SQQGPP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU641
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal FLAG tag