cDNA Clone MKK5

• Plasmid: pEBG MKK5 delta N11
• Parent Plasmid: pEBG2T
• Expressed: GST-MKK5 delta N11
• Genbank: MAP2K5 Mitogen-activated protein kinase kinase 5
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGAGAACCAGGTGCTGGTAATTCGCATCAAGATCCCAAATAGTGG
CGCGGTGGACTGGACAGTGCACTCCGGGCCGCAGTTACTCTTCAGGGATG
TGCTGGATGTGATAGGCCAGGTTCTGCCTGAAGCAACAACTACAGCATTT
GAATATGAAGATGAAGATGGTGATCGAATTACAGTGAGAAGTGATGAGGA
AATGAAGGCAATGCTGTCATATTATTATTCCACAGTAATGGAACAGCAAG
TAAATGGACAGTTAATAGAGCCTCTGCAGATATTTCCAAGAGCCTGCAAG
CCTCCTGGGGAACGGAACATACATGGCCTGAAGGTGAATACTCGGGCCGG
ACCCTCTCAACACAGCAGCCCAGCAGTCTCAGATTCACTTCCAAGCAATA
GCTTAAAGAAGTCTTCTGCTGAACTGAAAAAAATACTAGCCAATGGCCAG
ATGAATGAACAAGACATACGATATCGGGACACTCTTGGTCATGGCAACGG
AGGCACAGTCTACAAAGCATATCATGTCCCGAGTGGGAAAATATTAGCTG
TAAAGGTCATACTACTAGATATTACACTGGAACTTCAGAAGCAAATTATG
TCTGAATTGGAAATTCTTTATAAGTGCGATTCATCATATATCATTGGATT
TTATGGAGCATTTTTTGTAGAAAACAGGATTTCAATATGTACAGAATTCA
TGGATGGGGGATCTTTGGATGTATATAGGAAAATGCCAGAACATGTCCTT
GGAAGAATTGCAGTAGCAGTTGTTAAAGGCCTTACTTATTTGTGGAGTTT
AAAGATTTTACATAGAGACGTGAAGCCCTCCAATATGCTAGTAAACACAA
GAGGACAGGTTAAGCTGTGTGATTTTGGAGTTAGCACTCAGCTGGTGAAT
TCTATAGCCAAGACGTATGTTGGAACAAATGCTTATATGGCGCCTGAAAG
GATTTCAGGGGAGCAGTATGGAATTCATTCTGATGTCTGGAGCTTAGGAA
TCTCTTTTATGGAGCTTGCTCTTGGGAGGTTTCCATATCCTCAGATTCAG
AAAAACCAGGGATCTTTAATGCCTCTCCAGCTTCTGCAGTGCATTGTTGA
TGAGGATTCGCCCGTCCTTCCAGTTGGAGAGTTCTCGGAGCCATTTGTAC
ATTTCATCACTCAGTGTATGCGAAAACAGCCAAAAGAAAGGCCAGCACCT
GAAGAATTGATGGGCCACCCGTTCATCGTGCAGTTCAATGATGGAAATGC
CGCCGTGGTGTCCATGTGGGTGTGCCGGGCGCTGGAGGAGAGGCGGAGCC
AGCAGGGGCCCCCGTGAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSENQVLVIRIKIPNSGAVDWTVHSG
PQLLFRDVLDVIGQVLPEATTTAFEYEDEDGDRITVRSDEEMKAMLSYYY
STVMEQQVNGQLIEPLQIFPRACKPPGERNIHGLKVNTRAGPSQHSSPAV
SDSLPSNSLKKSSAELKKILANGQMNEQDIRYRDTLGHGNGGTVYKAYHV
PSGKILAVKVILLDITLELQKQIMSELEILYKCDSSYIIGFYGAFFVENR
ISICTEFMDGGSLDVYRKMPEHVLGRIAVAVVKGLTYLWSLKILHRDVKP
SNMLVNTRGQVKLCDFGVSTQLVNSIAKTYVGTNAYMAPERISGEQYGIH
SDVWSLGISFMELALGRFPYPQIQKNQGSLMPLQLLQCIVDEDSPVLPVG
EFSEPFVHFITQCMRKQPKERPAPEELMGHPFIVQFNDGNAAVVSMWVCR
ALEERRSQQGPP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU589
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression