cDNA Clone RAS

• Plasmid: pFBHTb RAS
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-RAS
• Genbank: P01112
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCACGGAATATAAGCTGGTGGTGGTGGGCGCCGGCGGTGTGGGCAA
GAGTGCGCTGACCATCCAGCTGATCCAGAACCATTTTGTGGACGAATACG
ACCCCACTATAGAGGATTCCTACCGGAAGCAGGTGGTCATTGATGGGGAG
ACGTGCCTGTTGGACATCCTGGATACCGCCGGCCAGGAGGAGTACAGCGC
CATGCGGGACCAGTACATGCGCACCGGGGAGGGCTTCCTGTGTGTGTTTG
CCATCAACAACACCAAGTCTTTTGAGGACATCCACCAGTACAGGGAGCAG
ATCAAACGGGTGAAGGACTCGGATGACGTGCCCATGGTGCTGGTGGGGAA
CAAGTGTGACCTGGCTGCACGCACTGTGGAATCTCGGCAGGCTCAGGACC
TCGCCCGAAGCTACGGCATCCCCTACATCGAGACCTCGGCCAAGACCCGG
CAGGGAGTGGAGGATGCCTTCTACACGTTGGTGCGTGAGATCCGGCAGCA
CAAGCTGCGGAAGCTGAACCCTCCTGATGAGAGTGGCCCCGGCTGCATGA
GCTGCAAGTGTGTGCTCTCCTGAGAATTC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSTEYKLVVVGAGGVGKSALTIQL
IQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMR
TGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAAR
TVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKLRKLNP
PDESGPGCMSCKCVLS

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU588
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His tag for baculovirus expression