cDNA Clone PreScission Protease

• Plasmid: pGEX4T1 PreScission Protease
• Parent Plasmid: pGEX4T1
• Expressed: GST PreScission Protease
• Genbank: -
• Tag: GST

Sequence of Insert

GGATCCGGTGGAGGACCAAACACAGAATTTGCACTATCCCTGTTAAGGAA
AAACATAATGACTATAACAACCTCAAAGGGAGAGTTCACAGGGTTAGGCA
TACATGATCGTGTCTGTGTGATACCCACACACGCACAGCCTGGTGATGAT
GTACTAGTAAATGGTCAGAAAATTAGAGTTAAGGATAAGTACAAATTAGT
AGATCCAGAGAACATTAATCTAGAGCTTACAGTGTTGACTTTAGATAGAA
ATGAAAAATTCAGAGATATCAGGGGATTTATATCAGAAGATCTAGAAGGT
GTGGATGCCACTTTGGTAGTACATTCAAATAACTTTACCAACACTATCTT
AGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAGCACCC
CCACTAACAGAATGATTCGTTATGATTATGCAACAAAAACTGGGCAGTGT
GGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTGGCGG
TAATGGAAGACAAGGATTTTCAGCTCAACTTAAAAAACAATATTTTGTAG
AGAAACAATGAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSGGGPNTEFALSLLRKNIMTITTSK
GEFTGLGIHDRVCVIPTHAQPGDDVLVNGQKIRVKDKYKLVDPENINLEL
TVLTLDRNEKFRDIRGFISEDLEGVDATLVVHSNNFTNTILEVGPVTMAG
LINLSSTPTNRMIRYDYATKTGQCGGVLCATGKIFGIHVGGNGRQGFSAQ
LKKQYFVEKQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX4T1 PreScission Protease54 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU493
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST tag for bacterial expression; Calc Mol Weight 46402 Da
• Low Temp: No